Placas ósseas corticais bovina: avaliações mecânicas e microbiológica após conservação em diferentes meios
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Date
2010-04-30
Authors
Melo Filho, Edson Vilela de
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Publisher
Universidade Federal do Espírito Santo
Abstract
The objective of this study was to evaluate the mechanical strength of bone plates yielded from bovine cortical bone, conserved in different solutions, and the efficiency of these solutions in the inhibition of microorganism s growth. 168 plates yielded from bovine tibiae were conserved in 98% glycerin (n=28), 150% saline solution (n=28), 300% sugar solution (n=28), Dakin solution (n=28), frozen in LN2 at - 196°C (n=28), or sterilized in ethylene oxide gas (n=28). After rehydratated in NaCl 0.9% solution for six hours, plates were subjected to tensile, compression, bending and twisting testing. Bone plates microbiological evaluation was carried out before and immediately after rehydratation, with or without enrofloxacin 0.5% addition. There was no significant difference (P<0.01) on resistance to the rupture point in plates conserved in the different solutions, frozen-thawed or sterilized. Microorganisms were isolated from plates conserved in satured salt solution, sugar satured solution, Dakin solution or frozen in LN2, before and after rehydratation. However, after the use of NaCl 0.9% solution added enrofloxacin 0.5%, microorganisms were not isolated. Therefore, bovine bone plates conserved in sugar or salt satured solution, 98% glycerin, Dakin solution, frozen in LN2 or sterilized in ethylene oxide gas present similar biomechanical tests results, and NaCl 0.9% solution added enrofloxacin 0.5% rehydratation inhibits microbial growth
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Keywords
Implants , Grafts , Biomaterials , Conservation solutions , Mechanical testing , Implantes , Enxertos , Biomateriais , Meios de conservação , Ensaios mecânicos
Citation
MELO FILHO, Edson Vilela de. Placas ósseas corticais bovina: avaliações mecânicas e microbiológicas após conservação em diferentes meios. 2010. 64 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal do Espírito Santo, Alegre, 2010.