Please use this identifier to cite or link to this item: http://repositorio.ufes.br/handle/10/6636
Title: Diversidade molecular e morfoagronômica de genótipos de feijoeiro (Phaseolus vulgaris L.) avaliados na região sul do Espírito Santo
Other Titles: Molecular diversity and morphoagronomic genotypes of bean (Phaseolus vulgaris L.) evaluated in the south of the Espírito Santo
Authors: Cabral, Pablo Diego Silva
Keywords: Feijão Espírito Santo (Estado);Feijão conservação da diversidade das plantas;Melhoramento genético;Germoplasma vegetal;Genética molecular;Microssatélites (Genética)
Issue Date: 22-Feb-2010
Publisher: Universidade Federal do Espírito Santo
Citation: CABRAL, Pablo Diego Silva. Molecular diversity and morphoagronomic genotypes of bean (Phaseolus vulgaris L.) evaluated in the south of the Espírito Santo. 2010. 107 f. Dissertação (Mestrado em Fitotecnica; Recursos Florestais) - Universidade Federal do Espírito Santo, Alegre, 2010.
Abstract: The bean (Phaseolous vulgaris L.) is an important crop in the socioeconomic aspect, it is a source of proteins and employed its cultivation, labor of family farming. The Brazil is the largest producer of beans and in the state, of the Espírito Santo that culture takes third place in importance. The yield is still low, but can be increased by correcting the improper management has adopted and using commercial seed adapted to each region. In this sense, it is necessary to know the genetic diversity between wild and commercial cultivars to support programs to improve the culture adapted to the conditions of specific regions. To estimate the genetic differences among 57 bean genotypes were performed three experiments, one in the agricultural year of 2008/09 and two in 2009/2010. Of the genotypes, 31 were rescued in the town of Fortaleza, 20 supplied by EMBRAPA and six cultivars. To estimate the genetic diversity were used morphological and molecular analysis. The morphological analysis were made using 19 quantitative characters and 27 qualitative, in turn, the molecular primers were used 16 primers of molecular markers SSR and 11 ISSR primers. The characters qualitative, quantitative and molecular techniques were effective in differentiating the genotypes, as evidenced by the value of cophenetic correlation coefficient (CCC), all of which were significant, at 1% probability by t test. The highest and lowest CCC were observed, respectively, the analysis by SSR (0.923) and ISSR (0.833). There wasn t correlation between the groupings made by the three types of characters. In the analysis by SSR markers, there were 14 groups, the ISSR, 11 and the combination of markers, 14. In the analysis of quantitative characters, we observed the formation of 4 groups and the qualitative, 11. This discrepancy in the number of groups formed expected, due to the difference in valuation of each character. There was wide variation among bean genotypes studied. In the genotypes from community Fortaleza (local) was a significant genetic diversity, but among the genotypes from EMBRAPA, the diversity was narrow. The analysis of quantitative traits showed that the cultivars studied was small dissimilarity with lower value (24.96) among the cultivars Iapar 81 and lapar 44, representing 1.08%. There wasn t identification of duplicates and genetic correlation of only 0.22 between the dissimilarity matrices obtained by SSR and ISSR markers show that these markers together reflected in greater efficiency in discriminating power of the genotypes. The combination of markers was more efficient in the separation of the genotypes, distinguishing difference between them, on the other, the separate analysis classified the genotypes as genetically identical. The correlation moderate / high (0.57) between the SSR markers and quantitative traits shows that the dissimilarity using SSR markers linked to desirable agronomic characteristics can replace the analysis of quantitative traits, since the analysis for these markers aren t affected by the environment as they are easy, fast and require less amount of manpower and physical space
URI: http://repositorio.ufes.br/handle/10/6636
Appears in Collections:PPGPV - Dissertações de mestrado

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