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Title: Caracterização de Pitcairnia flammea (Bromeliaceae), ocorrente em fragmentos de Mata Atlântica, por meio de ferramentas biotecnológicas
Other Titles: Genetic diversity in natural populations of Pitcairnia flammea and transferability of SSR and ISSR primers
Authors: Nogueira, Ester Ujiie
Keywords: Bromélia Biotecnologia;Bromélia Genética vegetal;Bromélia Recursos do germoplasma;Bromélia Melhoramento genético;Bromélia Mata Atlântica Conservação
Issue Date: 31-Mar-2010
Publisher: Universidade Federal do Espírito Santo
Citation: NOGUEIRA, Ester Ujiie. Genetic diversity in natural populations of Pitcairnia flammea and transferability of SSR and ISSR primers. 2010. 78 f. Dissertação (Mestrado em Fitotecnica; Recursos Florestais) - Universidade Federal do Espírito Santo, Vitória, 2010.
Abstract: RAPD molecular markers are useful for analyzing genetic diversity, enabling a broader sampling of unknown genomes. SSR and ISSR markers are also used for this purpose, but still only a few are developed to Bromeliaceae, which implies the validation of their transferability among species of this family. This study aims at studying the genetic variability of P. flammea by means of RAPD markers and testing the transposition of SSR and ISSR markers for this species. 8 RAPD primers were used in the study of diversity, and 4 ISSR primers and 1 SSR primer were used to validate the transferability of molecular markers. The 8 RAPD primers generated a total of 55 bands, 41 of which polymorphic. From the total molecular genetic variance found, 69.18% are due to the differences between individuals within each collection site, and 30.82% are due to differences between the three sites sampled. The genetic variation among populations, Gst=0.2340, has indicated great genetic proximity. The populations belonging to the geographically closest collection sites presented a smaller genetic distance between them (0.04), reflecting a greater genetic identity (0.9714). The greatest genetic variation was observed among the individuals sampled in the Collection Site III, so the existing population in this site may be considered a priority in conservation works. In the tests of transposition, 17 bands were obtained with ISSR markers, 6 of which being polymorphic. For the SSR marker, 1 monomorphic band was produced. RAPD markers were efficient for analyzing the intra and inter populational genetic diversity of P. flammea, and the transferability of SSR and ISSR markers was validated in P. flammea
Appears in Collections:PPGPV - Dissertações de mestrado

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