Mestrado em Ciências Fisiológicas
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início:
Conceito atual na CAPES:
Ato normativo:
Periodicidade de seleção:
Área(s) de concentração:
Url do curso:
Navegar
Navegando Mestrado em Ciências Fisiológicas por Autor "Andrich, Filipe"
Agora exibindo 1 - 2 de 2
Resultados por página
Opções de Ordenação
- ItemCaracterização da resposta inflamatória local induzida pela peçonha do peixe-escorpião Scorpaena plumieri(Universidade Federal do Espírito Santo, 2011-04-25) Menezes, Thiago Nunes de; Andrich, Filipe; Figueiredo, Suely Gomes de; Garcia, Maria Elena de Lima Perez; Bissoli, Nazaré SouzaEnvenomation with Scorpaena plumieri fish venom causes immediate, local, and intense pain, and tissue edema in humans. The aim of the present study was to characterize experimentally the local acute inflammatory response induced by S. plumieri venom (SpV). Mouse paw edema bioassays have showed that SpV (7.5 - 100 µg of protein) induce intense local edema and nociception. The edema intensity and its persistence were dose-dependent. It was also demonstrated that previous administration of serine-protease inhibitor (aprotinin) or bradykinin B2 receptor antagonist (HOE-140) significantly reduces the edematogenic response to venom. These results demonstrate a direct involvement of the Kallikrein-Kinin System in the edema formation. However, the pre-treatment with other anti-inflammatory drugs (antihistamine and inhibitor of COX-2) had less effect on this response. Elevated levels of proinflammatory mediators (cytokines TNF and IL-6 and the chemokine MCP-1) were detected in supernatants of paw tissue extracts by flow cytometric analyses 2h after SpV (15 µg of protein) injection. This proinflammatory mediators release could be associated with histopathological changes observed into paw tissue, characterized by cellular infiltration, mainly neutrophils (6h) followed later by mononuclear cell recruitment (12h after injection). It was also observed edema at strong dermis of the tissue injected. Thus, kinins, in concert with cytokines, may regulate leucocytes interstitial accumulation and trafficking to the inflammatory site. Partial purification of a proteic proinflammatory fraction, which reproduced the edematogenic response induced by crude venom, was achieved by gel filtration and anion exchange chromatographies. In addition also was demonstrated that stonefish antivenom (SFAV) is able to neutralize in vivo nociceptive and edematogenic activities of the venom of S. plumieri. Western immunoblotting analysis confirmed the cross reactivity, since SpV proteins with MW in the range of ~ 98 kDa were recognized by SFAV. These experiments suggest that the SpV compound responsible for the inflammatory effects possesses similar antigenic properties to those found in stonefish venom. In conclusion, this work described for the first time the pattern of local inflammatory reaction elicited by S. plumieri venom. It was also reported structural similarities between venom proteins from Indo-Pacific (stonefish) and Atlantic Ocean fish (scorpionfish).
- ItemEstudo das propriedades farmacológicas e bioquímicas da peçonha e do muco da pele do peixe-escorpião Scorpaena plumieri(Universidade Federal do Espírito Santo, 2013-10-25) Lemos, Pedro Henrique; Andrich, Filipe; Figueiredo, Suely Gomes de; Santos, Daniel Moreira dos; Rodrigues, Lívia Carla de MeloThe scorpionfish Scorpaena plumieri is one the most common venomous fish found along the Brazilian coast and is often involved in human accidents. Its venomous stings are covered by an integumentary sheath coated by a skin mucus layer, which may be carried along with the venom into the wound caused by the sting. This led us to suppose that the local and systemic manifestations induced by S. plumieri in accidents and experimental animals are combined effects of substances secreted by venom glands and epidermal cells. Therefore, the aim of this work was to continue the study S. plumieri venom (SpV) and initiate the physio-pharmacological and biochemical characterization of the skin mucus of this fish (SpSM), which has never been explored. In addition, a proteomic approach was employed to unravel and compare the complex composition of these two venoms. Both SpV and SpSM exhibit proteolytic activity upon gelatin and fibrinogen, albeit the hydrolysis was slow and non-specific. Hyaluronidase, cardiovascular, inflammatory and hemolytic activities were found only for SpV, evidencing the exclusive production of toxins (cytolysin and hyaluronidase) by the venom glands. The hemolysis seems to result from a direct action of venom constituents on erythrocytes membrane, since it lacks phospholipase A2 activity. Although devoid of hemolytic activity, SpSM was able to agglutinate rabbit erythrocytes (MHC 2.5mg/mL) suggesting the presence of carbohydrate-recognition proteins (lectins) in the mucus. Mass spectrometry analysis of the main fractions eluted from the two-dimensional liquid chromatography (gel filtration and reversed phase) revealed exclusive molecular species for SpV (533 = 36%) and SpSM (416 = 28%); 269 (18%) were found in both venoms. It was observed a predominance of mass lower than 10KDa in both SpV and SpSM (69% and 93%, respectively). The data here presented suggest that SpSM does not exhibit components capable to trigger relevant envenomation reactions. Nevertheless, together with SpV, it is a rich source of bioactive compounds, specially proteins and peptides