Avaliação de marcadores de remodelamento cardíaco (funcionais, bioquímicos e estruturais) em ratos submetidos a exercício resistido e tratamento com decanoato de nandrolona.
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Data
2015-07-13
Autores
Lima, Ewelyne Miranda de
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Universidade Federal do Espírito Santo
Resumo
Resistance training (RT) has often been associated with the abuse of anabolic androgenic steroids (AAS) by athletes and non-athletes. The aim of this study was to evaluate the effects of resistance training (RT) with or without nandrolone decanoate, with the levels of cytokines and activity of angiotensin-converting enzyme (ACE) in the heart, and the sensitivity of cardiopulmonary Bezold-Jarisch reflex (BJR). Male Wistar rats were divided into 3 groups: C (receiving vehicle, untrained); E (RT: after one week of adaptation water, rats were trained to jump into the water twice a week for 4 weeks), DN (treated with nandrolone decanoate 10 mg kg twice a week, intramuscularly) e DNE (treated with nandrolone decanoate 10 mg kg twice a week, intramuscularly, associated with RT). The BJR was analyzed by measuring bradycardic and hypotensive responses induced by administration in bolus of five serotonin randomized doses (2 to 32 g/kg body weight). Myocyte hypertrophy and collagen deposition in the matrix was determined by morphometric analysis of images from slides stained with H&E and picrosirius red respectively. The TNF-α levels in the heart and ACE activity was determined by Elisa assay. The RT promoted resting bradycardia in E group (bpm 312 ± 9, p <0.01) when compared with C group (360 ± 11 bpm) and ND did not influence this parameter NDE (bpm 324 ± 9, p <0.05), furthermore promoted physiological hypertrophy in myocytes without collagen deposition and no other changes were observed. The ND association with RT shown myocyte hypertrophy, type I collagen deposition, increase on TNF-α levels (C: 207 ± 9; E: 211 ± 8; DN: 269 ± 10; DNE: 242 ± 10 pg / mg, p <0,01) and ACE activity(C: 27,0 ± 7 ; E: 39,0 ± 6; DN: 50 ± 5; DNE: 65,0 ± 4 %, p <0,01), decreased IL-10 levels (C: 220 ± 7; E: 231 ± 5; DN: 165 ± 5; DNE: 164 ± 4 pg / mg, p<0,01), impairment in the sensitivity of BJR and hypertension(C: 104 ± 2 mmHg; E: 108 ± 8 mmHg; DN: 144 ± 7; DNE: 138 ± 5 mmHg; p <0,01). ND is associated with changes in cardiac structure and function as a result of the development of pathological cardiac hypertrophy (cytokine imbalance in heart elevation of ACE activity) and cardiac injury, and resistance training was unable to reverse the deleterious cardiovascular effects.
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Anabolic steroids , Angiotensin II , Cardiac hypertrophy , Cardiac remodeling , Cardiopulmonary reflex , Cytokines , Resistance training , Esteroides anabolizantes , Angiotensina II , Hipertrofia cardíaca , Remodelamento cardíaco , Reflexo cardiopulmonar , Citocinas , Treinamento resistido
Citação
LIMA, E. M., Avaliação de marcadores de remodelamento cardíaco (funcionais, bioquímicos e estruturais) em ratos submetidos a exercício resistido e tratamento com decanoato de nandrolona. 2015. Tese (Doutorado em Ciências Fisiológicas) - Universidade Federal do Espírito Santo, Centro de Ciências da Saúde, Vitória, 2015.