Associação da metilação dos genes NR3C1 e BDNF com os níveis de glicose e resistência à insulina em adultos atendidos pelo SUS
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Data
2022-02-23
Autores
Souza, Marcele Lorentz Mattos de
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Universidade Federal do Espírito Santo
Resumo
High blood sugar and insulin resistance are high risk conditions for mortality and contribute to the development of type II diabetes mellitus (T2DM). Studies on epigenetic modifications related to NR3C1 gene methylation focus on psychosocial stress. There are no studies on the association between epigenetic changes in the NR3C1 gene and DM2, especially related to glucose levels and insulin resistance. However, studies have shown the association of brain-derived neurotrophic factor (BDNF) levels in T2DM patients. The present study aimed to analyze the relationship between glucose levels and insulin resistance with changes in NR3C1 and BDNF genes methylation levels in adult individuals. This study included 375 individual users of the Brazilian Public Unified Health System (SUS), aged 20-59 years, and evaluated socioeconomic status, lifestyle, anthropometric data, blood glucose, insulin resistance, and evaluation of NR3C1 and BDNF genes DNA methylation. Factors associated with glucose levels and insulin resistance were investigated using multivariate analysis GLzM with a significance of 5% (p<0,05). Our results showed that glucose levels and insulin resistance were directly related to age and NR3C1 gene methylation and indirectly related to overweight and obesity and tobacco consumption. Regarding the BDNF gene, our results showed a positive relationship between gene methylation and glucose levels and insulin resistance, while non-overweight and obesity were inversely related to glycemia and insulin resistance. Habits and lifestyle can alter gene regulation through DNA methylation, showing the complexity of environmental impacts on NR3C1 and BDNF genes methylation. These data contribute to a better understanding of the pathophysiology and diagnosis of metabolic diseases involving the NR3C1 and BDNF genes. Furthermore, associated risk factors must be taken into account in epigenetic studies as they directly interfere with blood glucose levels and insulin resistance.
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metilação , epigenética , pirosequenciamento , TyG , estilo de v