Análise temporal do número e genotoxicidade de células-tronco de medula óssea de camundongos ateroscleróticos
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Data
2008-08-04
Autores
Tonini, Clarissa Loureiro
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Universidade Federal do Espírito Santo
Resumo
Currently stem cells have been target of many studies, many of them using cellular therapy in the cardiovascular diseases. However, little is known about the mechanisms that modify function and mobilization of the stem cells when submitted to the senescence and atherosclerosis. The analysis of stem cells of the ApoE-KO mice disclosed that atherosclerosis increases the number of mesenchymal and hematopoietic cells on the bone marrow probably through the increase of the symmetrical divisions stimulated by the progression of the atherosclerotic plaque. Analysis of control group showed increased number of hematopoietic stem cells and diminished number of mesenchymal stem cells. Genotoxicity assay revealed high level of DNA fragmentation in the mononuclear cells of the bone marrow in the atherosclerotic group that occurs because of the production of free radicals in atherosclerosis, what also it was evidenced by the senescence of the analyzed aortic root. In contrast, the C57 group presented low level of DNA fragmentation and, therefore low vascular senescence. We also observed that aging increased the number of hematopoietic stem cell in this group, that is, this lineage of cells does not loss the proliferative capacity, and it does not happen with the mesenchymal lineage.
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Atherosclerosis , Stem cells , Genotoxicity , Aterosclerose , Celulas – tronco , Genotoxicidade