Adequação e verificação de procedimentos para determinação de mercúrio em amostras de fígado e rim de rato utilizando a técnica CV-GF AAS
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Data
2014-08-24
Autores
Santos, André Eliziário dos
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Universidade Federal do Espírito Santo
Resumo
Mercury is an element that still needs constant monitoring because it is highly toxic at trace-level concentrations, with possibility exposure and contamination by a variety of forms present in the environment. Thus, the aim of this work was adapt and verify a simple and efficient procedure for mercury determination in Wistar rat tissues using cold vapor generation, pre-concentration in graphite furnace and analysis by atomic absorption spectrometry (CV-GF AAS). They were made comparison of amalgamation methods, procedure optimization, verification of merit figures and analysis of liver and kidneys of chronically treated rat. The best results were obtained using the hot block digestion method as sample pre-treatment and gold coated tube combined with and net of gold for amalgamation. It was necessary to use dichromate, isopropanol and simeticone pre-analysis. Procedure accuracy was verified through recovery tests at five concentration (between 92 and 114 %), enabling the use of the proposed procedure.
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Mercury , Preconcetration , Gold , Forno de grafite
Citação
SANTOS, André Eliziário dos. Adequação e verificação de procedimentos para determinação de mercúrio em amostras de fígado e rim de rato utilizando a técnica CV-GF AAS. 2014. 69 f. Dissertação (Mestrado em Química) - Universidade Federal do Espírito Santo, Centro de Ciências Exatas, Vitória, 2014.