Eletroacupuntura em coelhos submetidos à hipovolemia aguda : avaliação eletrocardiográfica, cardiovascular e microscópica dos sistemas nervoso central e renal
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2010-09-09
Autores
Salgado, Ana Elisa Pato
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Universidade Federal do Espírito Santo
Resumo
The effects of the electroacunpuncture of the acupoint Pc6 (Neiguan) and hipertonic saline solution of 7,5% over the electrocardiographic, cardiovascular and microscopic parameters of the CNS and kidneys in rabbits subdued to anesthetics by isofluorane and severe hypovolemia have been evaluated. 24 animals, distributed equally in four groups were used (GH, GE, GS and GC). After stabilization of the anesthetic plan, hypovolemia was induced in the animals with the scope of simulating severe hemorrhage, draining blood from the carotid artery until the average blood pressure reached 35 mmHg. After 30 minutes of hypovolemia, the animals in group GH received 4,0 mL/Kg of hypertonic saline solution in 7,5%, in the infusion speed of 1,0 mL/Kg/min; the ones in the GE group, received acupuncture on the acupoint Pc6, in the frequence of 20 Hz in square wave; the ones in the GS group received acupuncture in the Sham points and the ones in the GC group have not received any treatment. The following variables have been studied: body temperature; f; FC and electrocardiographic trace; PAM; PVC; PVP and serum lactate. The registration of the variables, except for the serum lactate, has started before the production of the hypovolemia and after the anesthetic stabilization of the animals (Mbasal), immediately after the remove of blood (M0), 3 minutes after blood collection (M3), 30 minutes from blood take out and before the beginning of the treatments (M30) and every 10 minutes until 60 minutes from the withdrawal of blood (M40, M50 e M60). Serum lactate has been evaluated in the M0, M3 and M30. After the registry of the variables, euthanasia has been performed to collect from the CNS and kidneys and posterior light microscopy evaluation. For the evaluation of the CNS and kidneys, degenerated neuron count has been performed (front lobe and hippocampus) and cerebellum piriformis neurons (Purkinje cells), and the quantification of the erythrocyte in the glomeruli and in the medullary region between the collection tubules of the kidneys. The statisc evaluation has been performed by profile analysis (P<0,05) and Kolmogorov-Smirnov (P<0,05).There has been no significant difference between the groups and the monets of the studied variables. However, electrocardiographic and cardiovascular alterations have been observed between the M40, M50 and M60 moments in relation to the Mbsal, observing increase in the PAM, PVC e blood lactate variables, and compensatory responses in the variables of the electrocardiogram. In the CNS, no significant differences related to the brain region between the studied groups have been observed. In relation to the kidney microscopy, there were no significant difference in relation to the quantity of erythrocytes between the glomeruli and kidney tubules. However, in the GE group a greater quantity of erythrocytes has been noticed. Thus, as a conclusion, the electro stimulation of the Pc6 acupoint and the hypertonic 7,5% solution do not cause alterations on the electrocardiographic and cardiovascular parameters; and electroacupuncture improves perfusion in the renal of rabbits subdued to severe hemorrhage.
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Choque , Colóide , Acupuntura , Animal
Citação
SALGADO, Ana Elisa Pato. Eletroacupuntura em coelhos submetidos à hipovolemia aguda: avaliação eletrocardiográfica, cardiovascular e microscópica dos sistemas nervoso central e renal. 2010. 79 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal do Espírito Santo, Centro de Ciências Agrárias.