Crescimento de mudas Coffea arabica L. em substrato com lodo de esgoto e avaliação de sua toxidade
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Data
2016-08-30
Autores
Gouvêa, Glaucia de Mello Cunha
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Universidade Federal do Espírito Santo
Resumo
The treatment of domestic sewage has caused the production of large quantities of solid waste, sewage sludge, being an environmental problem. Being source of essential nutrients for the development of agronomic interest plants is undoubtedly one of the best alternatives for final disposal. However, recycling of sewage sludge is safely occur to people and the environment since the waste can contain organic and inorganic compounds, such as toxic heavy metals, and pathogens. The objective of this study to analyze the growth of Coffea arabica L. seedlings in the substrate compound sewage sludge sanitized and the potential cytotoxic, genotoxic and mutagenic sludge through genetic toxicity bioassay Allium cepa. The experiment was conducted in greenhouse conditions. The substrates used were: soil (Horizon B ultisol) in 85% proportions for the treatment T1 plus 15% of cattle manure, 85% for T2 treatment with addition of 15% sanitized sewage sludge, 70% for treatment T3 plus 30% of sanitized sewage sludge, 55% for T4 treatment plus 45% of sanitized sewage sludge and 40% for T5 treatment plus 60% of sanitized sewage sludge, comprising five treatments with six replications. Growth variables were measured after the period of seedlings formation and subsequently the substrates. Toxicity analyzes were performed in root meristem cells of Allium cepa, with 5.000 cells were analyzed by direct contact treatment and solubilized samples and controls. All variables were negatively affected from the sewage sludge increment sanitized. The treatments with sewage sludge by direct contact showed cytotoxic, genotoxic and mutagenic effect. Treatments with solubilized sewage sludge showed genotoxic effect. The use of sewage sludge sanitized substrates for the formation of coffee seedlings was not efficient for reasons of toxicity, not favoring their growth
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Initial growth , Biosolids , Genotoxicity , Mutagenicity , Crescimento inicial , Biossólido , Genotoxicidade , Mutagenicidade