Análise imuno-histoquímica de hipóxia renal em modelo de isquemia/reperfusão tratado com fator estimulador de colônia de granulócitos
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2014-06-26
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Araujo, Isabela Bastos Binotti Abreu de
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Universidade Federal do Espírito Santo
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The kidney demonstrates a natural ability to repair itself after damage locations, however, after affected the chances of developing kidney damage increases. The pathophysiology of ischemia/reperfusion (IR) is complex because there are simultaneous occurrence of cell damage and inflammation. The decrease in amount of oxygen requires a system capable of avoiding its adverse effects and molecular machinery HIF (Hypoxia Inducible Factor), a complex, acts as a transcription factor for a number of genes since the regulation of cell proliferation and apoptosis by signaling to angiogenesis. The Granulocyte Colony-Stimulating Factor (G-CSF) is a glycoprotein known for its ability to promote the survival, proliferation and differentiation of cells stimulating the recovery effects resulting from the IR. In order to observe the influence of these proteins a semi-quantitative analysis of kidneys samples submitted or not to IR was performed using morphological microscopic descriptions and immunohistochemical, with statistical calculations and graphics were done in GraphPad Prism® software. Morphological analysis demonstrated characteristic IR lesions in untreated specimens: bubbles in tubular epithelium; vacuolization, tubular distalization and distal luminal congestion. Similarly, it was found in treaties, but in less advanced stages and in control animals, there were no tissue differences. Analyses of electron microscopy showed changes in the filtering barrier with concomitant loss of glomerular other features. In control animals the typical architecture was observed, while for the treated animals was noted preservation of barrier. The presence of HIF-1α in the contralateral kidneys showed significant when compared with ischemic and treated samples (p<0.05). The incidence of the same protein in ischemic kidneys showed no difference. Analyzing the VEGF protein was proven that in contralateral kidneys no statistical difference, however, in the ischemic kidney there is significance within the three groups (p<0.05). The correlation between these two proteins was not statistically significant. In relation to cell proliferation, cell death activity, all three groups were statistically significant (p<0.05). As regard the treatment was demonstrated the protective activity of the drug and possible molecular interaction with HIF, while activation of this protein confirms its metabolic pathway previously described.
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