Mestrado em Doenças Infecciosas

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http://repositorio.ufes.br/handle/10/17579

Nível: Mestrado Acadêmico
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Navegando Mestrado em Doenças Infecciosas por Autor "Araújo, Filipe Augusto Coimbra de"

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    Avaliação do uso de fatores de ressuscitação no sistema BACTEC MGIT 960 para detecção de Mycobacterium tuberculosis em amostras paucibacilares
    (Universidade Federal do Espírito Santo, 2024-10-24) Araújo, Filipe Augusto Coimbra de; Palaci, Moisés ; https://orcid.org/0000-0003-2013-6071; http://lattes.cnpq.br/2602694352713051; https://orcid.org/0000-0002-9256-7391; http://lattes.cnpq.br/3004156583114119; Ferrazoli, Lucilaine ; https://orcid.org/0000-0002-5389-7056; http://lattes.cnpq.br/8786839834967611; Tavares, Sarah Gonçalves; https://orcid.org/0000-0003-4170-4445; http://lattes.cnpq.br/1018708169217296
    The dormancy of Mycobacterium tuberculosis (Mtb) challenges laboratory diagnosis, as dormant subpopulations are not easily detected by conventional methods. The objective of this study was to evaluate the use of culture filtrate (CF) in the BACTEC MGIT 960 system to improve the detection of Mtb in 245 paucibacillary clinical samples from patients suspected of having tuberculosis (TB). The study compared samples inoculated in MGIT medium with and without the addition of Mtb CF. The results showed that the addition of CF increased the sensitivity of the test, particularly in extrapulmonary samples, with sensitivity and specificity for MGIT+CF being 97.50% and 97.86%, respectively. In comparison, the Xpert MTB/RIF Ultra presented a sensitivity of 60.53% and a specificity of 93.97%, while the egg-based medium showed 40% sensitivity and 91% specificity. Furthermore, the use of CF increased the frequency of contamination in liquid cultures and did not alter the time to positivity of MGIT. These findings suggest that the use of CF may be a promising tool for enhancing the accuracy of TB diagnosis in samples with low bacterial load