Biotecnologia
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Programa de Pós-Graduação em Biotecnologia
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URL do programa: http://www.biotecnologia.ufes.br/pt-br/pos-graduacao/PPGBIOTEC
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Navegando Biotecnologia por Autor "Agostini, Lidiane Pignaton"
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- ItemAlteração de expressão gênica em células mononucleares do sangue periférico humano submetidas à exposição com herbicida à base de glifosato(Universidade Federal do Espírito Santo, 2018-06-27) Agostini, Lidiane Pignaton; Louro, Iúri Drumond; Paula, Flávia de; Guimarães, Marco Cesar Cunegundes; Vasallo, Dalton Valentim; Errera, Flávia Ambroisi ValleGlyphosate is a post-emergent, non-selective and systemic herbicide. In the creating process of glyphosate-based herbicides (GBHs) such as Roundup®, surfactants are added to improve efficiency. The priority route of glyphosate’s degradation in soil results in aminomethylphosphonic acid (AMPA). Molecular responses to glyphosate were analyzed in some species of plants and in some vertebrates. In humans, it is not known exactly what risks and mechanisms of action would explain glyphosate’s toxicity reported in some experiments. The hypothesis is that fast exposure to Roundup® and AMPA leads to the differentiated expression of genes related to important cellular processes. Thus, the aim was identified these genes in human peripheral blood mononuclear cells when exposed to Roundup® and AMPA. The MTT [3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] test was performed in triplicates to evaluate cell viability and the choice of treatment conditions used in the microarray technique (GeneChip® Human Transcriptome Array 2.0, Affymetrix). Eight chips were used: 3 for controls, 3 for AMPA (10 mM) and 2 for Roundup® (0.05%). The exposure time was 3 hours. Using a p <0.05 and a fold-change of ≥1.5 and ≤ −1.5, there were 26 differentially expressed genes (DEGs) identified after Roundup® exposure (3h; 0.05%) and 5 DEGs after AMPA treatment (3h; 10 mM). DEGs after Roundup® treatment showed association with 33 Gene Ontology (GO) cellular processes (enrichment analysis), mainly related to regulation. Pathview web was used to identify the effect off DEGs in different pathways. Only genes differentially expressed in Roundup® treatment were included in the pathways. TNF, LTA, TAB2 and ATM genes are related to NF-kappa B signaling pathway; BCL2L11 and ATM genes to FoxO signaling pathway; SESN3 and ATM genes to p53 signaling pathway; and TNF, BCL2L11 and ATM genes to apoptosis. Our results suggest that Roundup® change expression pattern of a several genes associated with cell cycle control, cellular processes regulation and apoptosis.
- ItemAvaliação de polimorfismos dos genes ATM, TP53, BCL2 e TGFb relacionados com o prognóstico de pacientes com carcinoma epidermóide de cabeça e pescoço : relação com radiossensibilidade tumoral(Universidade Federal do Espírito Santo, 2014-02-26) Agostini, Lidiane Pignaton; Conforti, Adriana Madeira Álvares da Silva; Louro, Iúri Drumond; Silva, Magnus Régios Dias da; Guimarães, Marco Cesar CunecundesPolymorphisms in genes that control DNA repair, cell cycle, apoptosis and cytokine transcription, are pointed as putative prognosis and radiosensibility markers in Head and Neck Squamous Cell Carcinoma (HNSCC) patients. We have typed polymorphisms ATM IVS62+60G>A, ATM Asp1853Asn, TP53 Arg72Pro, BCL2 - 938C>A, TGFβ Pro10Leu and TGFβ -509C>T in order to establish correlations with prognosis and treatment response in HNSCC patients, using the PCR-RFLP technique. Genotyping was performed using peripheral blood DNA from 210 patients with oral and oropharyngeal tumors and 101 patients with larynx tumors. In patients with oral and oropharyngeal tumors submitted to radiotherapy tretament, genotype ATM IVS62+60AA increases local relapse risk (OR=4.43; CI=1.22-16.13) and alleles BCL2 -938C and TGFβ -509T are related with worse disease-specific survival (HR=0.46; CI=0.24-0.90 e HR=2.20; CI=1.12-4.29, respectively). In contrast, patients not submitted to radioation therapy, homozygous TP53 Pro72 increases risk of death (OR=2.65; CI=1.05-6.65). In irradiated larynx tumor patients, allele TGFβ Pro10 was associated with local relapse risk (OR=0.09; CI=0.02-0.53) and death (OR=0.18; CI=0.04-0.86), as well as with worse local disease-free survival and disease-specific survival (HR=0.13; CI=0.03-0.59 and HR=0.21; CI=0.07-0.60, respectively). Allele BCL2 -938C was associated with worse disease specific survival (HR=0.32; CI=0.12- 0.83). TGFβ -509T was associated with higher risk of death in non-irradiated larynx patients (OR=9.11; CI=1.51-27.91). Therefore, we suggest these polymorphisms as markers of prognosis and radiosensibility in these tumors.