Biotecnologia

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http://repositorio.ufes.br/handle/10/17565
Programa de Pós-Graduação em Biotecnologia

Centro: CCS
Telefone: (27) 3335 7447
URL do programa: http://www.biotecnologia.ufes.br/pt-br/pos-graduacao/PPGBIOTEC

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Navegando Biotecnologia por Autor "Alves, Lyvia Neves Rebello"

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    Herbicida à base de glifosato como potencial fator de risco para o câncer de mama: uma análise da expressão gênica, das modificações epigenéticas e do uso de epifármacos
    (Universidade Federal do Espírito Santo, 2024-08-26) Alves, Lyvia Neves Rebello; Santos, Eldamária de Vargas Wolfgramm dos; Louro, Iúri Drumond; https://orcid.org/0000-0001-5160-9615; https://orcid.org/0000-0001-5107-3689; Paula, Flávia de; Batitucci, Maria do Carmo Pimentel; Pereira, Fausto Edmundo Lima; Carvalho, Elizeu Fagundes de
    Breast cancer is the most common neoplasm in women worldwide, with both genetic and environmental factors playing a role in its development. Glyphosate, the active ingredient in widely used agricultural herbicides, is recognized as a potential carcinogen and endocrine disruptor, making it a candidate for inducing epigenetic modifications linked to breast cancer. This study investigates the effects of the glyphosate-based herbicide Roundup® on non-tumorigenic (MCF10A) and tumorigenic (MCF7 and MDA-MB-231) breast cell lines, focusing on the expression of key breast cancer-related genes. Additionally, the study examines the association with epigenetic modifications and the use of epidrugs (5-Aza-2′-deoxycytidine, 3-Deazaneplanocin A, and Trichostatin A) to reverse potential alterations, aiming to understand the risks and mechanisms of herbicide action. Results indicate that Roundup® affects cells through a non-estrogenic mechanism, impacting both hormone-dependent and -independent cell lines with varying toxic and proliferative effects depending on dose and exposure time. Moreover, it altered the expression of breast cancer-related genes such as BRCA1 and BRCA2 at low doses. The use of epigenetic modulators was able to reverse some Roundup®-induced changes, suggesting the herbicide's role in epigenetic modifications. Overall, these findings highlight the importance of understanding glyphosate-based herbicide mechanisms in humans, which could enable personalized prevention strategies to mitigate breast cancer risks.
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    Influência de polimorfismos dos genes GCK, TCF7L2 e LEPR maternos no peso do bebê : uma correlação clínica e molecular
    (Universidade Federal do Espírito Santo, 2017-04-20) Alves, Lyvia Neves Rebello; Louro, Iúri Drumond; Meira, Débora Dummer; Carvalho, Elizeu Fagundes de
    Birth weight is the main cause of neonatal mortality and morbidity and has long been used as an important public health indicator. Furthermore, birth weight can have longterm health consequences once it is closely related to the development of chronic diseases in adult life. Due to the significant role of maternal glucose concentration as a determinant factor of offspring birth weight, genes that alter glucose homeostasis are good candidates for genes that influences fetal growth, and thus birth weight. To evaluate the influence of maternal genetic variants in the offspring birth weight, three polymorphisms related to glucose metabolism were analyzed (GCK rs1799884, TCF7L2 rs7903146 e LEPR rs1137101) in 250 pregnant women who participates of a prospective cohort of Santo Antônio de Jesus – BA, Brazil, through the utilization of TaqMan® assays and the Polimerase Chain Reaction (PCR) Real Time technic. Samples genotypes were correlated with obstetrical results and clinical, anthropometrics and life habits data of the mother. No significant direct association was found between maternal polimorphisms and the offspring birth weight. This result may be due to sample particularities, especially in relation to ethnicity, since 84% of the analyzed samples are black or brown. It was possible to verify a significant association (p<0.05) between the birth weight and the variables sex, maternal BMI and gestational age for all the three polymorphisms. Moreover, associations among the LEPR rs1137101 maternal genotypes with gestational age (p=0.037) and drinking alcohol (p=0.04) were found. These results suggest that other factors, whether environmental or genetic, are more related with offspring birth weight than maternal genetic variants that are associated with the glucose metabolism.