Doutorado em Ciências Fisiológicas
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Navegando Doutorado em Ciências Fisiológicas por Autor "Alves, Leandro Miranda"
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- ItemAVALIAÇÃO DA EXPOSIÇÃO AO TRIBUTILESTANHO NOS PARÂMETROS MORFOLÓGICOS E METABÓLICOS EM RATOS EXPOSTOS AO FRIO(Universidade Federal do Espírito Santo, 2022-06-30) Merlo, Eduardo; Graceli, Jones Bernardes; https://orcid.org/0000000193494369; http://lattes.cnpq.br/3803670746263603; https://orcid.org/0000-0003-0155-1472; http://lattes.cnpq.br/; Rodrigues, Livia Carla de Melo; https://orcid.org/; http://lattes.cnpq.br/2084216553746326; Alves, Leandro Miranda; https://orcid.org/0000-0001-7832-8717; http://lattes.cnpq.br/9883547704448526; Santos, Roger Lyrio dos; https://orcid.org/0000-0003-4316-7196; http://lattes.cnpq.br/1122196233280741; Lima, Leandro Ceotto Freitas; https://orcid.org/0000-0001-9550-6095Tributyltin (TBT) chloride is an endocrine-disrupting chemical linked with several metabolic complications. Brown adipose tissue (BAT) is emerging as a therapeutic target for metabolic complications. However, few studies have evaluated the TBT effect on BAT function. This study assessed whether a TBT exposure (100 ng/kg/day for 15 days via gavage) could modulate BAT morphophysiology. We evaluated the BAT parameters at room temperature (23ºC) and after a cold tolerance test (CTT – 6ºC) conditions. Interestingly, were observed low T4 levels in TBT rats under normal temperatures. A reduction in body temperature was observed in both conditions in TBT rats, suggesting an abnormal thermogenic function. BAT morphology irregularities were observed in TBT rats. Specifically, an increase in BAT lipid accumulation and unilocular adipocyte number. TBT also reduced lipid droplets and multilocular adipocyte number at room temperature. All these parameters were opposite in the CTT condition. Inflammation signals were observed in the TBT BAT. Collagen accumulation in TAM increased in the ambient condition, exacerbating in the cold condition in TBT rats. ROS production increased under both conditions. Finally, we observed negative correlations between body temperature and BAT lipid accumulation, BAT lipid accumulation and T4 levels, and multilocular adipocyte number. Conversely, and positive correlation was observed between BAT lipid accumulation, inflammation, and ROS production. Thus, these data suggest that the subacute and low dose of TBT exposure impaired BAT morphophysiology linked with lipid accumulation, inflammation, fibrosis, and oxidative stress in male rats.
- ItemEXPOSIÇÃO A UMA DIETA COM ALTO TEOR DE CARBOIDRATOS REFINADOS LEVA A ANORMALIDADES METABÓLICAS E REPRODUTIVAS, SEMELHANTES A CARACTERÍSTICAS DA SÍNDROME DO OVÁRIO POLICÍSTICO E FALÊNCIA OVARIANA EM RATAS(Universidade Federal do Espírito Santo, 2022-01-17) Nino, Oscar Mauricio Santamaria; Graceli, Jones Bernardes; https://orcid.org/0000000193494369; http://lattes.cnpq.br/3803670746263603; https://orcid.org/; http://lattes.cnpq.br/; Alves, Leandro Miranda; https://orcid.org/; http://lattes.cnpq.br/; Santos, Roger Lyrio dos; https://orcid.org/; http://lattes.cnpq.br/1122196233280741; Lima, Leandro Ceotto Freitas; https://orcid.org/; http://lattes.cnpq.br/; Santos, Leonardo dos; http://lattes.cnpq.br/4132087001362623Obesity is associated with female reproductive complications, such as polycystic ovary syndrome (SOP), is linked with obesity and insulin resistance (IR). The exact mechanism of this relationship remains unclear. We identified a Few studies that used refi
- ItemPotencial toxicológico do cádmio em dose semelhante à de exposição ocupacional na gênese de complicações no trato reprodutivo de ratas wistar(Universidade Federal do Espírito Santo, 2023-02-16) Costa, Charles Santos da; Graceli, Jones Bernardes; https://orcid.org/0000000193494369; http://lattes.cnpq.br/3803670746263603; https://orcid.org/0000-0003-3616-8211; http://lattes.cnpq.br/8647659013623563; Alves, Leandro Miranda; https://orcid.org/0000-0001-7832-8717; http://lattes.cnpq.br/9883547704448526; Padilha, Alessandra Simão; https://orcid.org/0000-0002-9585-1347; http://lattes.cnpq.br/7658998034219799; Santos, Roger Lyrio dos; https://orcid.org/0000-0003-4316-7196; http://lattes.cnpq.br/1122196233280741; Lima, Leandro Ceotto FreitasCadmium (Cd) is a toxic heavy metal known to be a major endocrine disruptor. However, few studies have explored the effects of Cd exposure on developing polycystic ovary syndrome (PCOS) features and premature ovarian failure (POF). In this study, we evaluated whether subacute exposure to Cd at a dose similar to that of occupational exposure results in damage to the hypothalamic-pituitarygonadal (HPG) axis and abnormalities related to PCOS and FOP. Wistar rats were exposed to CdCl₂ (100 ppm in water for 30 days), we evaluated the levels of Cd in the blood, HPG axis and uterus. In addition, we evaluated metabolic factors, HPG axis function, reproductive tract morphophysiology, inflammation, oxidative stress and fibrosis. Exposure to Cd raised its levels in serum, HPG axis and uterus. The Cd rats presented metabolic damage, adiposity reduction, dyslipidemia and insulin resistance (IR). Cd also caused the HPG axis to malfunction. Irregularities in the estrous cycle, abnormal hypothalamic mRNA expression with overexpression of Kisspeptin 1 (Kiss1), androgen receptor (AR) and rapamycin mechanistic target protein (mTOR) and lower expression of Kiss1 (Kiss1R) and leptin receptors (LepR) were observed and tumor necrosis factor alpha (TNF-a), high levels of luteinizing hormone (LH), low levels of anti-Müllerian hormone (AMH) and abnormal ovarian follicular development, reduced ovarian reserve and follicle number antral were observed, suggesting ovarian depletion. Furthermore, exposure to Cd decreases the granulosa layer thickness and the corpora lutea counting and increases the cystic and atretic follicles. Cd exposure increased inflammation, oxidative stress, and remodeling in the reproductive tract. Finally, positive correlations were observed between serum and reproductive tract Cd levels with insulin resistance, dyslipidemia and estrous cycle duration, cystic and atretic follicles, LH levels and inflammation. Thus, these data suggest that subacute exposure to Cd at a dose similar to that of occupational exposure alters the function of the HPG axis, leading to PCOS and FOP features besides metabolic abnormalities in female rats.