Ciências Fisiológicas

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Navegando Ciências Fisiológicas por Assunto "2K1C hypertension"

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    Efeitos do tratamento com alisquireno e L-arginina sobre a sensibilidade barorreflexa e estresse oxidativo de ratos com hipertensão renovascular
    (Universidade Federal do Espírito Santo, 2015-07-01) Mengal, Vinícius; Abreu, Glaucia Rodrigues de; Gouvea, Sonia Alves; Bissoli, Nazaré Souza; Futuro Neto, Henrique de Azevedo
    Renovascular hypertension is characterized by increased angiotensin II, oxidative stress, and by endothelial dysfunction. The purpose of this study was tested if the administration of aliskiren (ALSK) and L-arginine (L-ARG), would restore impaired baroreflex sensitivity and reduce the oxidative stress in a rat renovascular hypertension model. Hypertension was induced by clipping the left renal artery, and the following 5 groups were divided: SHAM, 2-kidney, 1-clip (2K1C); 2K1C plus ALSK (ALSK); 2K1C plus L-ARG (L-ARG); and 2K1C plus ALSK+ L-ARG (ALSK+LARG). SOD-2 and Catalase expression was measured in plasma and tissues and Assay of advanced oxidation protein products. After 21 days of treatment, only the ALSK+L-ARG group was effective in normalize the arterial pressure ( 108.8±2.8 mmHg). The L-ARG and ALSK+L-ARG groups not show hypertrophy of the left ventricle. All treatments restored the depressed baroreflex sensitivity to values found by SHAM group in both parasympathetic and sympathetic component of baroreflex system. Acute administration of TEMPOL restored the depressed baroreflex sensitivity in 2K1C group to values that resembles to those presented by other groups. The advanced oxidation protein products levels in plasma that 2K1C group was significantly increased in 2K1C group compared with SHAM, ALSK, L-ARG and ALSK+L-ARG groups (5,79±0,67 vs. 3,79±0,41; 3,96±0,35; 4,26±0,47 and 3,91±0,36 umol/Lchloramine-T respectively, P<0.05). The similar responses was found in cardiac tissue. The SOD-2 and Catalase expression in the cardiac tissue was significantly increased in ALSK, L-ARG and ALSK+L-ARG. The gp91phox in the cardiac tissue to L-ARG and ALSK+L-ARG groups was significantly decreased when compared with 2K1C and ALSK groups. In conclusion, combined ALSK+L-ARG treatment normalizes SBP and prevents ventricular hypertrophy in 2K1C hypertensive rats, an effect that can be partially attributed to increased baroreflex sensitivity and decreases oxidative stress.
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    Efeitos do tratamento combinado com alisquireno e L-arginina sobre a reatividade vascular em anéis de aorta de ratos com hipertensão renovascular
    (Universidade Federal do Espírito Santo, 2014-11-28) Santuzzi, Cintia Helena; Abreu, Glaucia Rodrigues; Mauad, Helder; Futuro Neto, Henrique de Azevedo; Bissoli, Nazare Souza; Lemos, Virginia Soares
    Angiotensin II is a key player in pathogenesis of renovascular hypertension and this condition is associated with endothelial dysfunction. We investigate aliskiren, Larginine and association of treatments on blood pressure (BP) and vascular reactivity in aortic rings. Hypertension was induced in male Wistar rats by clipping the left renal artery and animals were divided: Sham, 2-kidney, 1-clip (2K1C) hypertension, 2K1C+aliskiren (ALSK), 2K1C+L-arginine (L-arg), and 2K1C+aliskiren and L-arginine (ALSK+L-arg) treatment for 4 weeks. Blood pressure was monitored and endothelium-dependent and independent vasoconstriction and relaxation were assessed in aortic rings. ALSK+L-arg decreased blood pressure, contractile response to phenylephrine and improved the acetylcholine relaxation. Endothelium removal and incubation with L-NAME increased the response to phenylephrine in all groups, but magnitude was higher in ALSK+L-arg group. Enalapril reduced phenylephrine response in 2K1C and ALSK groups and Losartan reduced contractile response in all of the groups. Apocynin reduced contractile response in 2K1C, ALSK and ALSK+L-arg groups and incubation with superoxide dismutase (SOD) reduced phenylephrine response in 2K1C and ALSK groups. eNOS expression increase in 2K1C and L-arg groups and iNOS increased only in 2K1C group compared with other groups. AT1 expression increase in 2K1C compared to Sham, ALSK and ALSK+L-arg groups, AT2 expression increase in ALSK+L-arg group compared to Sham and L-arg groups, Ms SOD2 increased in ALSK+L-arg group compared to 2R1C, ALSK and Larg group, on the other hand, gp91phox decreased in ALSK+L-arg compared with 2K1C and ALSK. In conclusion, combined ALSK+L-arg was effective in reducing BP and preventing endothelial dysfunction in aortic rings of 2K1C hypertensive rats and the mechanisms responsible appear to be related to the modulation of local reninangiotensin system, which is associated with the reduction in endothelial oxidative stress.