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Activity of prokaryotic communities from offshore oil reservoir and their adaptation to high hydrostatic pressure
(Universidade Federal do Espírito Santo, 2023-02-15) Santos, Luiza Favarato; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; https://orcid.org/0000000173409358; http://lattes.cnpq.br/1217579607633423; Fernandes, Antonio Alberto Ribeiro; https://orcid.org/0000-0003-0535-9349; http://lattes.cnpq.br/4696507759154477; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; Rodrigues, Silas Pessini; https://orcid.org/0000-0002-8672-3626; http://lattes.cnpq.br/9067991933812274; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; Biasi, Ronaldo Sergio de
Deep-sea microbes comprise a significant portion of Earth’s biomass, but they are still poorly studied. Although the subseafloor is an oligotrophic, anoxic, dark, and pressurized environment, life still thrives in such kind of environment. In this work, three samples collected 100 m, 3,060 m, and 6,000 m deep in the Brazilian coast were investigated by analysis of 16S rRNA. A difference was found in the biological diversity between these communities at the genus level. The three communities had acid-producing bacteria and sulfate-reducing bacteria, but only the community from 3,060 m deep had a methanogenic archaeon. From the 6,000 m deep sample was isolated a bacterium of the Halanaerobium genus. Two important variables for the environment and offshore industries, bacterial survival, and hydrogen sulfide (H2S) production under high hydrostatic pressure (HHP) were investigated. Cell survival and H2S production were affected by HHP. In the case of the 3,060 m bacteria, population and H2S production increase with increasing pressure, while for the 100 m bacteria, population is stable with increasing pressure but H2S production decreases. The results show the importance of studying the influence of pressure on bacterial growth and H2S production to define strategies to mitigate economic losses arising from activities in marine environments.
Análise da composição química e da atividade antibacteriana e antifúngica de clones de Schinus terebinthifolia Raddi
(Universidade Federal do Espírito Santo, 2023-03-01) Moraes, Rodrigo; Ventura, Jose Aires; https://orcid.org/0000-0003-1422-1739 ; http://lattes.cnpq.br/8687116881326074 ; https://orcid.org/0000-0001-9175-4825; http://lattes.cnpq.br/0598172220327707; Santos, Alexandre Martins Costa; https://orcid.org/0000-0002-8801-8875; http://lattes.cnpq.br/4144105396879016; Endringer, Denise Coutinho; https://orcid.org/0000-0001-9396-2097; http://lattes.cnpq.br/6424432214565576; Kuster, Ricardo Machado; https://orcid.org/0000-0002-8961-5348; http://lattes.cnpq.br/4149814906786366; França, Hildegardo Seibert ; https://orcid.org/0000-0001-6129-8793; http://lattes.cnpq.br/1284874997224988
Aroeira (Schinus terebinthifolia Raddi) is included in the Brazilian List of Essential Medicines due to its potential to generate bioactive compounds, including those with antimicrobial properties. The occurrence of antimicrobial resistance increases health care costs, length of stay in hospitals, morbidity, and mortality. WHO has published a list of antibiotic-resistant “priority pathogens” to guide and promote research, development, and innovation of new antibiotics including those that may combat oxacillin-resistant Staphylococcus aureus and carbapenemase-producing Klebsiella pneumoniae. Fungi of the genus Fusarium are important pathogens for plants, humans, and animals, in addition to producing mycotoxins in food, with a great lack of new molecules that can be used for their control. This research sought to characterize the phytochemical profile of polar and non-polar extracts of mature fruits of four Aroeira clones and their antimicrobial activity against oxaxilin-resistant Staphylococcus aureus, carbapenemase-producing Klebsiella pneumoniae and the fungus Fusarium guttiforme. The extracts of all aroeira clones showed antimicrobial activity for bacteria and fungus, varying according to the clone and solvent used. Polar extracts showed greater antifungal and antibacterial activity, being more active on Gram-positive bacteria. The antifungal and antibacterial activity varied according to the evaluated clone. Clone BAAr 5 showed greater antibacterial activity while clones BAAr 5 and BAAr 8 had greater antifungal activity. The Aroeira clones showed similarity in the chromatographic profile by HPLC. It was possible to observe a greater relative number of peaks in clone BAAr 5 when extracted using a hydroethanolic solvent. The chromatogram of the hexane extracts showed a greater diversity of peaks. There was a greater abundance of sesquiterpenes compared to monoterpenes when the samples were evaluated in GC-MS. Spectrometry using ESI(-)FT-ICR MS identified the presence of 29 ions, between m/z 195 and m/z 925 in the polar extracts of the clones. Twenty-four ions were observed in clone BAAr 5, 20 in clone BAAr 8, 23 in clone BAAr9 and 17 in clone BAAr 23. Although the clones were grown under the same edaphoclimatic conditions, they showed variation in antimicrobial activity and bioactive compounds identified. It is suggested that the commercial use of Aroeira should come from selected and cloned plants that present the desired characteristics
ANÁLISE DA EXCITABILIDADE E CONECTIVIDADE CORTICAL PARA UM SISTEMA DE NEURORREABILITAÇÃO BASEADO EM INTERFACE-CÉREBRO MÁQUINA E MONOCICLO ROBÓTICO
(Universidade Federal do Espírito Santo, 2021-06-29) Cardoso, Vivianne Flavia; Filho, Teodiano Freire Bastos; https://orcid.org/0000000211852773; http://lattes.cnpq.br/3761585497791105; https://orcid.org/; http://lattes.cnpq.br/; Nogueira, Breno Valentim; https://orcid.org/0000000221990635; http://lattes.cnpq.br/0011229320439147; Andrade, Adriano de Oliveira; https://orcid.org/; http://lattes.cnpq.br/; Caldeira, Eliete Maria de Oliveira; https://orcid.org/; http://lattes.cnpq.br/; Neto, Anselmo Frizera; https://orcid.org/0000000206873967; http://lattes.cnpq.br/8928890008799265; Rodriguez, Denis Delisle; https://orcid.org/000000028937031X; http://lattes.cnpq.br/7140331839822423
CARDOSO V.F. 2021. 110f. Analysis of excitability and connectivity cortical for a neurorehabilitation system based on brain machine interface and robotic monocycle. 2021. 121f. Thesis (doctoral in biotechnology)- Postgraduation Biotechnological Program, U
Avaliação da expressão de microRNAs e proteínas como biomarcadores de diagnóstico em carcinoma epidermoide de língua
(Universidade Federal do Espírito Santo, 2023-11-22) Có, Anna Clara Gregório; Camillo, Cláudia Malheiros Coutinho; https://orcid.org/0000-0001-9016-2668; http://lattes.cnpq.br/3184503163639480; Zeidler, Sandra Lúcia Ventorin von; https://orcid.org/0000-0002-8897-5747; http://lattes.cnpq.br/5785612863130498; https://orcid.org/0000-0002-7737-0371; http://lattes.cnpq.br/3678557620411441; Nunes, Fabio Daumas; https://orcid.org/0000-0002-7785-6785; http://lattes.cnpq.br/4909755821591847; Errera, Flavia Imbroisi Valle; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531; Paula, Flavia de; https://orcid.org/0000-0001-8679-2982; http://lattes.cnpq.br/7913201450663683
Oral squamous cell carcinoma (OSCC) is the 16th most commonly diagnosed form of cancer globally, with a higher prevalence in the tongue compared to other areas of the oral cavity. However, the lack of effective biomarkers for diagnosis, especially for precancerous lesions, poses a limitation, as visual or histological examination cannot predict the progression of dysplastic lesions, making it difficult to determine whether they will develop into cancer or return to normal epithelium. In this context, the present research aims to investigate molecular targets that may indicate the irreversible transformation of these cells, to provide a basis for broader studies aimed at using these targets as biomarkers for early OSCC diagnosis. To achieve this goal, this experimental study addressed the evaluation of the expression of a panel of microRNAs and proteins in tumour tissues and adjacent tumour-adjacent epithelium obtained from patients with tongue squamous cell carcinoma and oral epithelium from healthy individuals. Additionally, the research explored the association between these biomarkers, seeking to determine their potential application as diagnostic biomarkers for tongue squamous cell carcinoma. A total of 75 cases of tongue squamous cell carcinoma and adjacent tumouradjacent epithelium were included in the study, and the expression of the proteins survivin, Bcl-2, PLK1, p16, p40, p63, EGFR, and cyclin D1 was analysed by immunohistochemistry. The analysis of the microRNA panel's expression involved 31 samples of tongue squamous cell carcinoma, 10 samples of healthy gingival tissue, and 10 samples of serum from healthy individuals, as well as 7 samples of serum from patients diagnosed with OSCC, using the RT-qPCR technique. In silico analysis by bioinformatics validated the findings related to the expression of differentially expressed microRNAs in the sample group. The results showed differences in the expression of miRNA-31-5p (p<0.001) and miRNA-21-5p (p=0.001) in tumour samples compared to control samples. Significant differences were not observed in the expression of miRNA-24-3p, while miRNA-542-3p and 196a-5p were not detected in the sample group. No significant difference was observed in the expression of miRNAs in serum samples. The assessment of the diagnostic potential of microRNAs included ROC curve analysis, which revealed that miR-21-5p had an area under the curve (AUC) of 0.803, while miR-31-5p obtained an AUC of 0.777. The results also identified differential expression among the proteins survivin, PLK1, and p63, all of which showed increased expression in tumour tissue. Additionally, a correlation was observed between the expression of miR-21-5p and the protein p40 (chi-square: p=0.047; Spearman correlation: r=0.402; p=0.023). In conclusion, the results suggest that miR-21-5p and miR-31-5p may be potential diagnostic biomarkers for tongue squamous cell carcinoma, providing a foundation for further exploration for large-scale studies to explore miRNA-protein correlations, considering the site specificity of miRNAs.
Avaliação do potencial bioativo de frações peptídicas de coprodutos de peroá (balistes capriscus)
(Universidade Federal do Espírito Santo, 2024-04-30) Ribeiro, Monique Lopes; Santos, Alexandre Martins Costa; https://orcid.org/0000-0002-8801-8875; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Cicilini, Maria Aparecida; https://orcid.org/; http://lattes.cnpq.br/; Oliveira, Jairo Pinto de; https://orcid.org/; http://lattes.cnpq.br/; Koblitz, Maria Gabriela Bello; https://orcid.org/; http://lattes.cnpq.br/; Souza, Bartolomeu Warlene Silva de; http://lattes.cnpq.br/
Fish industry generates a significant amount of waste which have high biological value and potential for industrial use. The aim of this study was to evaluate the antioxidant and ACE-inhibitory activities of peptide fractions from triggerfish (Balistes capriscus) processing coproducts. Molecular mass distribution of the proteins extracted from fish viscera was determined by molecular exclusion chromatography presenting fractions with a wide range of molecular mass (<1.2 kDa to > 440 kDa). Soluble Protein Extract (SPE) was hydrolysate using papain (HP), bromelain (HB) and trypsin (HT) (3% p.p-1, 6h). Samples were fractionated (>100 MWCO, 30–100, 10-30 and < 10 MWCO). Antioxidant activity of fractions was evaluated and fraction SPE4 (<10 MWCO) showed the highest value of Trolox Equivalent Antioxidant Capacity - TEAC (10,157.7 μmol Trolox. g-1) and Ferric Reducing Antioxidant Power - FRAP (1,588.71 μmol FeSO4. g-1 ). SPE and hydrolysates (<10MWCO) were separated by ion-exchange chromatography. Fraction F1 showed the highest value for TEAC capacity (8,839.04 μmol Trolox. g-1) and FRAP (1,749.94 μmol FeSO4. g-1). ACE-inhibitory activity was evaluated for non-hydrolysate and hydrolysate fractions. Fractions F3, F5 and HP3 showed the lowest IC50 value (30.1, 42.7 e 37.7 µg, respectively). Antimicrobial activity was observed in samples SPE (against S. aureus), F1 and F4 (against Pseudomonas sp.) It was identified 20 amino acid sequences that could contribute to the biological activity of the peptide fractions. Proteins extracted from triggerfish viscera demonstrated to be a good source of bioactive peptides that may have food and pharmaceutical applications.
“CARACTERIZAÇÃO DO PADRÃO DE MUTAÇÕES DA OSTEOGÊNESES IMPERFEITA E AVALIAÇÃO IN VITRO DOS EFEITOS DE ANTIOXIDANTES RESVERATROL, ÁCIDO ASCÓRBICO E CURCUMINA EM CÉLULAS-TRONCO MESENQUIMAIS”
(Universidade Federal do Espírito Santo, 2022-02-03) Trancozo, Maira; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; https://orcid.org/0000000185623482; http://lattes.cnpq.br/; Batitucci, Maria do Carmo Pimentel; https://orcid.org/; http://lattes.cnpq.br/0010148251489155; Maranduba, Carlos Magno da Costa; https://orcid.org/; http://lattes.cnpq.br/; Meira, Debora Dummer; https://orcid.org/; http://lattes.cnpq.br/; Nishimura, Agnes Lumi
Osteogenesis Imperfecta (OI) is an inherited disorder of connective tissues that contain collagen in their formation. More than 20 OI-related genes have been reported in the last years. Most cases happen due mutations inherited with autossomic dominant in
Caracterização físico-química de hidrolases de proteoforma de tripsina bovina e lipases de resíduos pesqueiros
(Universidade Federal do Espírito Santo, 2023-12-18) Cruz, Fabiano Torres; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; https://orcid.org/0000-0003-2510-5354; http://lattes.cnpq.br/2548521743413029; Teixeira, Kadima Nayara; Oliveira, Jairo Pinto de; https://orcid.org/0000000175951183; http://lattes.cnpq.br/2228283301316218; Koblitz, Maria Gabriela Bello; Cicilini, Maria Aparecida
Hydrolases have significant biotechnological importance, with lipases and proteases being highlighted as essential catalysts in industrial biotechnological processes, playing fundamental roles in the production of food, beverages, biofuels, and pharmaceuticals, with positive impacts both economically and environmentally. Integrating two chapters dedicated to the detailed study of trypsin and lipase, the research aims to deepen the understanding of their properties and contributions to the evolution of biotechnology. Notable advances in enzyme research have revealed a new proteoform of bovine trypsin, showing significant differences in activity, kinetic properties, and conformational stability compared to other known forms, opening new perspectives for understanding physiological pathways of enzyme inactivation. In the context of lipases, the study focuses on the economic and environmental potential of lipases derived from tilapia viscera, exploring purification, enzyme shelf life, and economic and environmental improvements. The results indicate that lipases from tilapia viscera can be purified and become viable alternatives with economic and environmental potential for Brazil.
Desenvolvimento de biomaterial ósseo descelularizado enriquecido com hidrogel de osso fetal bovino
(Universidade Federal do Espírito Santo, 2024-10-10) Barros, Rodolpho José da Silva; Nogueira, Breno Valentim ; https://orcid.org/0000-0002-2199-0635; http://lattes.cnpq.br/0011229320439147; https://orcid.org/0000-0001-6214-733X; http://lattes.cnpq.br/5652361736715531; Maranduba, Carlos Magno da Costa ; https://orcid.org/0000-0001-7327-1934; http://lattes.cnpq.br/4763153859701731; Oliveira, Jairo Pinto de ; https://orcid.org/0000-0001-7595-1183; http://lattes.cnpq.br/2228283301316218; Guimarães, Marco Cesar Cunegundes ; https://orcid.org/0000-0003-2146-0180; http://lattes.cnpq.br/0261991057482057; Aires, Rafaela; https://orcid.org/0000-0002-9532-9401; http://lattes.cnpq.br/5449202639864996
The global bone graft material market is projected to grow at a rate of 6-7% annually until 2032. Conventional bone grafting strategies are no longer considered sufficient or adequate for most treatments. The development of decellularized biomaterials derived from animal tissues (xenografts) offers a promising and viable strategy. The structural similarity between animal and human bones, along with the decellularization technique, can result in a high-quality biomaterial that provides mechanical support, preserves the organic matrix, and supplies essential biological stimuli for tissue regeneration. Adult and fetal bovine bones were decellularized to produce biomaterials (blocks, granules, and hydrogel) with residual DNA levels lower than 50 ng/mg and SDS levels below 2 μg/mg of dry tissue. Thermogravimetric assays (DSC and TGA) and infrared spectroscopy (FTIR and Raman) demonstrated the adequate preservation of both the mineral and organic bone matrices. Scanning electron microscopy (SEM) and elemental composition analysis (EDS and CHNS-O) confirmed the preservation of the microstructure and biochemical composition of the bone matrix. pH stability, absence of heavy metals, and sterility were verified. Comparative tests showed that the decellularized biomaterials, particularly those derived from fetal bone, exhibit superior porosity, density, mechanical support capacity, and organic composition compared to other biomaterials. In vitro tests revealed no cytotoxicity and a higher ability to stimulate the proliferation of mesenchymal cells and fibroblasts. The results indicate that the production of decellularized bovine bone biomaterials coated with fetal hydrogel could contribute to better biocompatibility of implants and help meet the global demand for bone graft materials
Detecção de beauvericina por HPLC-DAD e MALDI-FT ICR MS em frutos de abacaxi infectados por Fusarium guttiforme
(Universidade Federal do Espírito Santo, 2024-06-27) Fávero, Carolinne Simões; Romão, Wanderson ; https://orcid.org/0000-0002-2254-6683; http://lattes.cnpq.br/9121022613112821; Ventura, José Aires ; https://orcid.org/0000-0003-1422-1739; http://lattes.cnpq.br/8687116881326074; https://orcid.org/0000-0002-0915-0350; http://lattes.cnpq.br/6385055031220477; Errera, Flavia Imbroisi Valle ; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; França, Hildegardo Seibert ; https://orcid.org/0000-0001-6129-8793; http://lattes.cnpq.br/1284874997224988; Costa, Helcio ; https://orcid.org/; http://lattes.cnpq.br/2086091514383462; Pereira, Fausto Edmundo Lima ; http://lattes.cnpq.br/4065537941002091
Pineapple is a fruit of great economic importance in fruit farming in Brazil and of high consumption worldwide, used in human and animal food, among other uses. Among the phytosanitary factors limiting the crop, fusariosis stands out, a disease caused by the fungus Fusarium guttiforme, which compromises the quality of the fruits for the market and produces an emerging mycotoxin called beauvericin (BEA), potentially harmful to the health of consumers. The importance of accurate and validated techniques for detecting BEA and establishing maximum allowable limits is urgent, as there is still no regulation in legislation in any country. Analytical techniques for the detection of mycotoxins have proven to be effective, with few studies for the detection of BEA in pineapple. Reliability in detection allows careful selection of evaluated products, ensuring food safety and economic responsibility. High Performance Liquid Chromatography (HPLC), Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) combined with the matrix-assisted laser desorption/ ionization (MALDI) source and UHPLC-MS (Ultra Efficiency Liquid Chromatograph coupled to a mass spectrometer) were used to detect BEA in this work. The rice substrate was tested for the development of F. guttiforme and production of BEA, as it is a favorable environment for the development of the fungus. The HPLC-DAD results, in the first analyses, made it possible to detect BEA in the tissues of samples visibly infected with F. guttiforme (E-514), however in samples of apparently healthy tissue the mycotoxin was not detected. With MALDI-FT-ICR MS, BEA was detected in both types of samples, showing that, in addition to the greater sensitivity of the method, there is the possibility that the mycotoxin may diffuse into the apparently healthy tissues of fruits inoculated with F. guttiforme, with these tissues also being contaminated, but at limits not detectable by HPLC-DAD. The rice substrate proved to be efficient for the detection of beauvericin produced by isolates of F. guttiforme cultivated in vitro, which allows its use to produce the mycotoxin on a larger scale for subsequent studies. After these first results, UHPLC-MS was used for further analyses, however the answers were not satisfactory. In studies for the detection of BEA in pineapples previously inoculated with F. guttiforme (E-514), the analytical method that proved to be most accurate, until regulatory limits were met, was MALDI-FT-ICR MS, showing extremely high sensitivity of the method for mycotoxin detection and the ability to detect very small quantities not detected in HPLC-DAD
Determinação do perfil molecular dos extratos de folhas de Carica papaya (L.) em busca de biomarcadores estágio-dependentes relacionados à doença da meleira do mamoeiro
(Universidade Federal do Espírito Santo, 2024-07-12) Britto, Isabella Oliveira; Santos, Alexandre Martins Costa; https://orcid.org/0000-0002-8801-8875; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Leite, João Paulo Viana; https://orcid.org/; http://lattes.cnpq.br/; Silva, Diolina Moura ; https://orcid.org/; http://lattes.cnpq.br/; Gonçalves, Juliana Barbosa Coitinho; https://orcid.org/; http://lattes.cnpq.br/; Lima, Graziela Domingues de Almeida; http://lattes.cnpq.br/
Papaya Sticky Disease is caused by PMeV viral complex and poses a significant threat to papaya production worldwide. Infected plants remain asymptomatic until flowering and fructification, acting as silent reservoirs of the virus in the field. Secondary metabolites could act as potential biomarkers of disease progression and can be detected using chromatographic techniques for early disease diagnosis. In this study, molecular profiles of ethanolic extracts from C. papaya leaves in pre- and postflowering (2.5, 5.0, and 7.5 mg.mL-1) and fractions were evaluated by UV-Vis spectroscopy, reversed-phase chromatography, ion exchange chromatography, and mass spectrometry. The 7.5 mg.mL-1 concentration was selected as the experimental concentration for crude extracts. Chromatograms showed symmetrical peaks, eluted within similar retention time ranges across the three plant stages, with narrow base widths, similar peak shapes, and no tailing, suggesting the presence of a limited number of potentially isolable compounds. Analytical parameters indicated quantitatively larger chromatographic peaks in pre-flowering leaf extracts compared to post-flowering ones, reflecting a possible defense response against viral infection. Significant differences in chromatographic profiles between the different plant stages were observed, indicating the potential of these groups as biomarkers for Papaya Sticky Disease. This study developed a sensitive and reproducible chromatographic method to distinguish plant stages, identifying flavonoids and alkaloids as components of strategic groups related to the defense system against viral infections.
Diversidade e frequência haplotípica de X-STRS na população do Espírito Santo e sua contribuição para elucidação de casos forenses complexos
(Universidade Federal do Espírito Santo, 2023-03-03) Rodrigues, Fernanda Mariano Garcia de Souza; Louro, Iuri Drumond; https://orcid.org/0000000151609615; http://lattes.cnpq.br/3817361438227180; https://orcid.org/0000000270355005; http://lattes.cnpq.br/0479360701927218; Meira, Debora Dummer; https://orcid.org/0000-0002-6092-2459; http://lattes.cnpq.br/7199119599752978; Carvalho, Elizeu Fagundes de; https://orcid.org/0000-0003-4620-7253; http://lattes.cnpq.br/2742420738858309; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; Rave, Cintia Fridman
Genetic markers Short Tandem Repeats (STR) are the center of human genetic identification, STR markers on autosomal chromosomes and on the Y chromosome being the most used. However, in some forensic situations, such as cases of suspected incest, paternity without a maternal sample for comparison, traces with mixed DNA, the use of only these markers may not be enough to solve these cases. The study of STRs markers of the X sex chromosome (X-STRs) significantly increases the probability of identification by complementing the data obtained for autosomal and Y chromosome markers. Statistical analyzes that must be included in the issuance of expert reports. Thus, the general objective of this work is to carry out a macro-regional survey of the haplotype frequencies of 12 loci of X-STRs in Espírito Santo, to estimate the genetic diversity, the dynamics of populations and promote the gain of statistical power and the updating of this information in databases international data. In this sense, the survey of haplotype frequencies of X-STRs was carried out with a sample group of 571 unrelated individuals born in the 4 macro-regions of the state, in order to obtain a reliable estimate of the genetic diversity of ES. Analyzing the set of 12 XSTRs, no statistically significant differences were found between the macro regions of the state. The allele and haplotype frequencies found here have high rates of allelic and haplotype variability, demonstrating that this X-STR set is very informative in terms of individual discrimination, and may contribute to building knowledge and the importance of using the X chromosome in routine of laboratories that use DNA technology in human identification.
Dynamics of the papaya meleira virus complex during the development of papaya (Carica papaya L.)
(Universidade Federal do Espírito Santo, 2021-11-11) Araujo, Marlonni Maurastoni; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; https://orcid.org/0000000260643126; http://lattes.cnpq.br/; Aragao, Francisco Jose Lima; https://orcid.org/; http://lattes.cnpq.br/; Fernandes, Antonio Alberto Ribeiro; https://orcid.org/; http://lattes.cnpq.br/4696507759154477; Junior, Francisco Murilo Zerbini; https://orcid.org/; http://lattes.cnpq.br/; Rodrigues, Silas Pessini; Withfield, Anna Elizabeth
MAURASTONI, M. A. Dynamics of the papaya meleira virus complex during the development of papaya (Carica papaya L.). 2021. 173p. Thesis for the Degree of Ph.D. in Biotechnology Postgraduation Biotechnology Programme, UFES, Espirito Santo. Brazil. Among t
Estudo de associação das variantes dos genes TCF7L2 e ADIPOQ com diabetes, obesidade e longevidade em uma coorte de idosos de São Paulo
(Universidade Federal do Espírito Santo, 2021-08-11) Bride, Lais de Lima; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; https://orcid.org/0000-0003-3703-2631; http://lattes.cnpq.br/4221199792517120; Bastos Filho, Teodiano Freire; https://orcid.org/0000000211852773; http://lattes.cnpq.br/3761585497791105; Maranduba, Carlos Magno da Costa; https://orcid.org/0000-0001-7327-1934; http://lattes.cnpq.br/4763153859701731; Errera, Flavia Imbroisi Valle; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; Paneto, Greiciane Gaburro; https://orcid.org/0000000180354199; http://lattes.cnpq.br/8176374147579841; Luizon, Marcelo Rizzatti; https://orcid.org/0000-0002-8331-3525; http://lattes.cnpq.br/1264026443614775
Type 2 diabetes mellitus (DM2) and obesity are complex diseases directly related to the quality and expectancy of life of elderly across the world in the current century. There are several reports that genetic factors play a crucial role in the risk for complex diseases. Therefore, the investigation of genetic biomarkers is an important biotechnological tool for the prevention and treatment of complex diseases, also for better healthy aging and lifespan. The TCFL72 and ADIPOQ genes encode important proteins participating in metabolic processes and these genes have been widely described by association studies. Thus, the main objectives of this study were to evaluate the association of the rs7903146 polymorphism in TCF7L2 gene with DM2 and obesity, as also the relation of the SNPs rs17300539 (-11391G/A), rs266729 (- 11377G/C), rs2241766 (+45T/G) and rs1501299 (+276 G/T) in ADIPOQ gene with longevity status. More than 1,000 participants from an elderly cohort named SABE (Saúde, Bem Estar e Envelhecimento - Health, Welfare and Aging) were evaluated over 10 years (2000 to 2010) and the anthropometric, clinical and genetic data were collected during this period. The TCF7L2 and ADIPOQ genotypes were extracted from the ABraOM (Online Archive of Brazilian Mutations) database. Logistic regression models and additional statistical analysis were performed and the main findings were: the association of TCF7L2 rs7903146 T allele with the risk for DM2 in inverse relation to the BMI status; the TCF7L2 rs7903146 C allele increased the risk for obesity; Association of the ADIPOQ variants rs2241766 with longevity and rs1501299 with protection for longevity; The “T/T” haplotype from rs2241766 and rs1501299 variants showed association with less chance for longevity, whereas the “G/G” haplotype from the same variants was positively associated with longevity risk. When gender was considered, we detected stronger associations of rs7903146 SNP with diabetes in men, while more significant values concerning the association of ADIPOQ variants with longevity were observed in women. Thus, the present study confirms the TCF7L2 rs7903146 SNP association with diabetes and obesity and that ADIPOQ variants are associated with longevity status in our studied cohort.
Evaluating the effect of the simultaneous cerebrospinal stimulation, motor imagery, virtual reality and pedaling on post-stroke patients
(Universidade Federal do Espírito Santo, 2024-09-16) Mehrpour, Sheida; Andrade, Adriano de Oliveira ; https://orcid.org/0000-0002-5689-6606; http://lattes.cnpq.br/1229329519982110; Bastos Filho, Teodiano Freire ; https://orcid.org/0000-0002-1185-2773; http://lattes.cnpq.br/3761585497791105; https://orcid.org/0000-0002-1217-8071; http://lattes.cnpq.br/4006017652838495; Espírito Santo, Caroline Cunha; https://orcid.org/0000-0001-8657-9532; http://lattes.cnpq.br/4920759696380516; Rodríguez, Denis Delisle ; https://orcid.org/0000-0002-8937-031X; http://lattes.cnpq.br/7140331839822423 ; Fernandez, Antônio Alberto Ribeiro ; https://orcid.org/0000-0003-0535-9349; http://lattes.cnpq.br/4696507759154477; Nogueira, Breno Valentim ; https://orcid.org/0000-0002-2199-0635; http://lattes.cnpq.br/0011229320439147
Technology in medicine is transforming the healthcare landscape by enhancing diagnostics, treatment, and patient management. With the integration of advanced tools and systems, healthcare professionals can deliver more accurate and timely care. Innovations such as telemedicine, artificial intelligence, and electronic health records streamline processes and improve communication among providers. Additionally, technology facilitates personalized medicine, allowing treatments to be tailored to individual patients based on their unique needs. The ongoing evolution of medical technology not only increases efficiency but also expands access to healthcare, ensuring that patients receive the best possible outcomes. As technology continues to advance, its role in medicine will become even more pivotal in shaping the future of healthcare. Stroke is the leading cause of acquired physical disability in humans, and the second largest cause of global mortality. Technology in stroke rehabilitation plays a vital role in enhancing recovery outcomes for patients. Advanced tools such as virtual reality, robotics, Brain-Computer Interface based on Motor Imagery (BCI-MI), Non Invasive Brain Stimulation (NIBS) techniques, and telehealth platforms offer innovative ways to engage patients in their rehabilitation process. Virtual reality can simulate real life scenarios, helping patients practice daily activities in a safe environment, while robotic exoskeletons assist in retraining motor functions through repetitive movements. Telehealth enables remote therapy sessions, providing continuous support and flexibility for patients to engage in their recovery from home. Additionally, wearable devices allow for real-time monitoring of progress, ensuring that treatment plans can be adjusted to meet individual needs effectively. Overall, these technological advancements are reshaping stroke rehabilitation, making it more personalized, accessible, and efficient. Non-Invasive Brain Stimulation (NIBS) techniques, such as transcranial direct current stimulation (tDCS) and transcutaneous spinal Direct Current Stimulation (tsDCS), are increasingly being applied in stroke rehabilitation to enhance recovery outcomes. These methods work by modulating neuronal activity in targeted brain regions, promoting neuroplasticity and facilitating motor function recovery. By improving communication between brain areas affected by the stroke and those responsible for movement, NIBS can help patients regain lost skills more effectively. As research continues to advance, these techniques hold promise for optimizing rehabilitation strategies and improving the quality of life for stroke survivors. The main objective of this study is to develop new, low-cost rehabilitation methods to patients with subacute to chronic stroke, aiming to increase neuroplasticity and improve motor function through combining methods such as tDCS plus tsDCS, VR, MI and pedaling exercise. This research are divided into three separate Chapters to assess both the long-term effects (Chapter I) and the immediate effects (Chapters II and III) of the intervention. In chapter I, the study was set up with the Alternative Treatment Design (ATD), comprising three phases: baseline, sham stimulation, and real stimulation. For Chapters II and III, the study design was defined as a pre- and post-stimulation assessment. For the experiment in the first Chapter, four subacute hemiparetic stroke patients were selected. The same experiment and participants were recruited for Chapters two and three, but the methodology for evaluating the effects of the intervention differed between these Chapters. For Chapters two and three, a total of eight participants were selected, including four patients and four healthy individuals. In both experiments, participants were randomly assigned to two groups to receive cerebrospinal stimulation, according to two different protocols (conventional and periodic). Participants in the conventional stimulation group received 20 minutes of stimulation, while those in the periodic stimulation group underwent two 13-minute stimulation sessions separated by a 20-minute rest period. The anode electrode was placed over the M1 region of the affected hemisphere, guided by the 10/20 International System. The cathode electrode was positioned centrally on the spinous process of the thoracic vertebra at T11 (T10-T12) by palpation. For the first experiment the results were evaluated using surface electromyography (sEMG), Maximum Voluntary Contraction (MVC), Fugl-Meyer Assessment for Lower Extremity (FMA-LE), miniBESTest, goniometry, 10-meter walk test (10MWT), pedaling speed, as well as specific stroke scales. In the second experiment, in addition to stimulation, Virtual Reality was used to enhance Motor Imagery (MI) effect in order to evaluate the combined effect on Mu and Beta bands modulation in post-stroke patients and healthy individuals. Results from the second experiment were analyzed using quantitative electroencephalography (EEG) measures, such as cortical topography based on mean amplitude values, brain connectivity parameters such as Phase Locking Value (PLV) and Magnitude Squared Coherence (MSC). For Chapter III, the Hjorth parameters (activity, mobility, complexity) were used across two assessment sessions, pre- and post-stimulation. The results of the experiment presented in Chapter I indicated significant improvements in muscle contraction, motor function and gait among patients. Participants in the conventional stimulation protocol group showed enhancements in tibialis muscle contraction, as assessed by sEMG and ankle dorsiflexion goniometry. In contrast, those in the periodic stimulation protocol exhibited improvements in motor function measures such as FML-LE, MiniBestest, and the 10- meter walk. Findings from the Chapter II experiment revealed different patterns of brain connectivity under the combined effects of cerebello-spinal stimulation, along with VR and MI, in both patients and healthy controls, emphasizing the need for personalized treatments for post-stroke patients. Results of Chapter III showed that the beta band is more sensitive to modulation by the combined methods compared to the Mu band, which was more reactive in patients than in healthy controls. The Activity parameter had a greater influence on the modulation of Mu and Beta bands in both patients and healthy controls. While the Mobility parameter showed greater influence in patients, the Complexity parameter was more sensitive in healthy controls. Due to the variability of the results and the small sample size, it was challenging to distinguish the effects of the two stimulation protocols in Chapters II and III
FATORES DETERMINANTES DA DOR CRÔNICA E O PAPEL DA METILAÇÃO DO GENE NR3C1
(Universidade Federal do Espírito Santo, 2021-08-30) Branco, Alexandre Lima Castelo; Louro, Iuri Drumond; https://orcid.org/0000000151609615; http://lattes.cnpq.br/3817361438227180; https://orcid.org/0000-0002-1704-9877; http://lattes.cnpq.br/7332472547330240; Guimarães, Marco Cesar Cunegundes; https://orcid.org/0000000321460180; http://lattes.cnpq.br/0261991057482057; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531; Carvalho, Marcos Brasilino de; https://orcid.org/0000-0001-6854-2680; http://lattes.cnpq.br/6208433886573740; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108
Chronic pain is a multidimensional health condition with high prevalence in Brazil and its chronic condition may be related to depression and anxiety, diseases recognized as the most prevalent mental disorders in the world and major causes of functional incapacity, suffering and reduced quality of life. The relationship between depression, anxiety, pain, suffering and epigenetic alterations have already been described in the literature, but this relationship is not completely clear yet. Epigenetic alterations can affect gene expression and are related to the individual's adaptation to the environment in a relationship between genotype, phenotype and environment. The glucocorticoid receptor gene, NR3C1, is regulated by epigenetic mechanisms and acts to control the neuroendocrine axis via cortisol, which also links the gene to depression and other psychiatric illnesses. Thus, this research evaluated the determinants of chronic pain, biopsychosocial, biochemical and molecular factors in the epigenetic modifications of the NR3C1 gene in adults aged between 20 and 59 years, users of the Brazilian Unified Health System. The results of the biopsychosocial assessment in the sample showed a profile of people over 40 years old, with lower per capita income and education, low levels of cortisol, more reports of stress and anxiety, higher consumption of continuous medications, less physical activity and more prevalence of chronic pain. Pain was related to a statistical model that pointed out pain-related variables. Thus, the research showed indicators that point to a predominant profile of individuals with chronic pain, with determinant factors being: hypomethylation of the DNA of the NR3C1 gene in CpG 42, age over 40 years and low cortisol.
Fluorescência da clorofila a como indicador de porta enxertos de manga ‘Ubá’ mais tolerantes ao déficit hídrico
(Universidade Federal do Espírito Santo, 2020-02-13) Silva, Leonardo Faria; Silva, Diolina Moura; https://orcid.org/000000033885280X; http://lattes.cnpq.br/0341541450627705; https://orcid.org/0000000192822818; http://lattes.cnpq.br/; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; Araujo, Wagner Luiz; https://orcid.org/; http://lattes.cnpq.br/; Ventura, Jose Aires; https://orcid.org/0000000314221739; http://lattes.cnpq.br/8687116881326074; Nesi, Adriano Nunes
In order to select the photochemical parameters extracted from the chlorophyll a fluorescence kinetics as being the simplest, fastest and most efficient for early identification of the most vigorous rootstock in the formation of drought tolerant 'Ubá' see
Herbicida à base de glifosato como potencial fator de risco para o câncer de mama: uma análise da expressão gênica, das modificações epigenéticas e do uso de epifármacos
(Universidade Federal do Espírito Santo, 2024-08-26) Alves, Lyvia Neves Rebello; Santos, Eldamária de Vargas Wolfgramm dos; Louro, Iúri Drumond; https://orcid.org/0000-0001-5160-9615; https://orcid.org/0000-0001-5107-3689; Paula, Flávia de; Batitucci, Maria do Carmo Pimentel; Pereira, Fausto Edmundo Lima; Carvalho, Elizeu Fagundes de
Breast cancer is the most common neoplasm in women worldwide, with both genetic and environmental factors playing a role in its development. Glyphosate, the active ingredient in widely used agricultural herbicides, is recognized as a potential carcinogen and endocrine disruptor, making it a candidate for inducing epigenetic modifications linked to breast cancer. This study investigates the effects of the glyphosate-based herbicide Roundup® on non-tumorigenic (MCF10A) and tumorigenic (MCF7 and MDA-MB-231) breast cell lines, focusing on the expression of key breast cancer-related genes. Additionally, the study examines the association with epigenetic modifications and the use of epidrugs (5-Aza-2′-deoxycytidine, 3-Deazaneplanocin A, and Trichostatin A) to reverse potential alterations, aiming to understand the risks and mechanisms of herbicide action. Results indicate that Roundup® affects cells through a non-estrogenic mechanism, impacting both hormone-dependent and -independent cell lines with varying toxic and proliferative effects depending on dose and exposure time. Moreover, it altered the expression of breast cancer-related genes such as BRCA1 and BRCA2 at low doses. The use of epigenetic modulators was able to reverse some Roundup®-induced changes, suggesting the herbicide's role in epigenetic modifications. Overall, these findings highlight the importance of understanding glyphosate-based herbicide mechanisms in humans, which could enable personalized prevention strategies to mitigate breast cancer risks.
Identificação de cochonilhas em cafeeiros utilizando DNA Barcoding e High Resolution Melting e distribuição potencial de Planococcus citri no Espírito Santo
(Universidade Federal do Espírito Santo, 2023-01-26) Oliveira, Pablo Viana; Paneto, Greiciane Gaburro; https://orcid.org/0000000180354199; http://lattes.cnpq.br/8176374147579841; https://orcid.org/000000021785178X; http://lattes.cnpq.br/9623188128443119; Serrão, José Eduardo; https://orcid.org/0000-0002-0477-4252; http://lattes.cnpq.br/6663553463256293; Telles, Mariana Pires de Campos; https://orcid.org/0000-0002-9023-0007; http://lattes.cnpq.br/4648436798023532; Ferreira, Marcia Flores da Silva; https://orcid.org/0000-0003-1541-6634; http://lattes.cnpq.br/5719813884063445; Ventura, José Aires; https://orcid.org/0000000314221739; http://lattes.cnpq.br/8687116881326074
Mealybugs (Hemiptera: Pseudococcidae) are agricultural pests of several crops, which can transmit viruses and cause great economic losses. For the control and management of these pests is essential a rapid and accurate identification of these insects. In this study, a rapid and low-cost method for identifying mealybug species was developed. The objectives are presented in the Results and Discussion section written in three chapters in Scientific Paper format. The first chapter presents an article accepted for publication in the Diversity journal, entitled "Molecular Species Delimitation Using COI Barcodes of Mealybugs (Hemiptera: Pseudococcidae) from Coffee Plants in Espírito Santo, Brazil". The dataset containing mitochondrial COI gene sequences of 26 putative of species Pseudococcidae recorded in Brazil were explored using the ASAP, GMYC, mPTP delimitation methods. An incongruence between the methods was observed, with the number of species ranging from 22 to 30 putative species. Of these, 10 species were identified among the new specimens collected in Brazil. In the second chapter, a paper to be submitted in a Journal with Impact Factor (≥ 3) is presented, entitled "Development of a High-Resolution Melting method based on COI minibarcodes to identify mealybug (Hemiptera: Pseudococcidae) pest species". A 76 bp primer pair was designed based on COI gene sequence alignment for 5 species of mealybugs. Analysis was performed by PCR immediately followed by HRM and the 5 species tested were discriminated, including two closely related species, Planococcus citri and Pl. minor. In addition, intraspecific variation was also detected in Pl. citri. The third chapter presents a paper for submission to a Journal with an Impact Factor (≥ 3), entitled "Potential distribution of the citrus mealybug, Planococcus citri (Risso, 1813), an important pest from coffee tree in Brazil”. The most abundant species found in coffee trees in Espirito Santo was chosen as a model for potential distribution prediction. Species distribution modelling was performed based on MaxEnt. North and northwest of the state was revealed to have the highest potential occurrence of this pest. This assumption was confirmed with our collection data, presented in the first chapter. This thesis presented important results on the diversity of species of mealybugs occurring in ES and another brazilian states. The tools used will help in the rapid identification of these insects and can be implemented in strategies for prophylaxis, monitoring and control of quarantine pests of agricultural crops in Brazil and other countries.
INFECÇÃO POR PAPILOMAVÍRUS HUMANO E EXPRESSÃO DAS PROTEÍNAS P16 E EGFR COMO BIOMARCADORES DE PROGNÓSTICO EM CARCINOMA EPIDERMOIDE ORAL E OROFARÍNGEO
(Universidade Federal do Espírito Santo, 2020-03-30) Abreu, Priscila Marinho de; Zeidler, Sandra Lucia Ventorin Von; https://orcid.org/0000000288975747; http://lattes.cnpq.br/5785612863130498; https://orcid.org/0000000164537171; http://lattes.cnpq.br/; Rotta, Rejane Faria Ribeiro; https://orcid.org/; http://lattes.cnpq.br/; Rabenhorst, Silvia Helena Barem; https://orcid.org/; http://lattes.cnpq.br/; Paneto, Greiciane Gaburro; https://orcid.org/0000000180354199; http://lattes.cnpq.br/8176374147579841; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; Podesta, Jose Roberto Vasconcelos de
Oral and oropharyngeal squamous cell carcinoma has as main risk factors the consumption of tobacco, alcohol and infection by Human Papillomavirus (HPV). Studies indicate that HPV-positive patients have results more favorable in relation to survival and re
INFLUÊNCIA MITOCONDRIAL NA REGULAÇÃO DE GENES ENVOLVIDOS COM ENVELHECIMENTO EM Saccharomyces cerevisiae SUBMETIDAS À ALTA PRESSÃO HIDROSTÁTICA
(Universidade Federal do Espírito Santo, 2020-02-07) Spagnol, Brigida de Almeida Amorim; Fernandes, Patricia Machado Bueno; https://orcid.org/0000000326953638; http://lattes.cnpq.br/2441925791593067; https://orcid.org/; http://lattes.cnpq.br/; Lomeli, Monica Montero; https://orcid.org/; http://lattes.cnpq.br/; Fernandes, Antonio Alberto Ribeiro; https://orcid.org/; http://lattes.cnpq.br/4696507759154477; Santos, Alexandre Martins Costa; https://orcid.org/0000000288018875; http://lattes.cnpq.br/4144105396879016; Pinto, Nadja Cristhina de Souza
Saccharomyces cerevisiae is a well-accepted eukaryotic model organism for studies on stress response and aging, WHEREas preserves several biochemical, molecular and organelle functioning mechanisms. Mitochondria is an organelle involved with stress respon

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