Doutorado em Química

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17501

Nível: início
Ano de início: 2014
Conceito atual na CAPES: 5
Ato normativo: Homologação da 85ª Reunião do CTC-ES, Parecer CNE/CES nº 163/2005.
Processo nº 23001.000081/2005-56 do Ministério da Educação.
Publicado no DOU 28/07/2005, seção 1, página 11)
Periodicidade de seleção: Anual
Área(s) de concentração: Química
Url do curso: https://quimica.vitoria.ufes.br/pt-br/pos-graduacao/PPGQ/detalhes-do-curso?id=956/a>

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Navegando Doutorado em Química por Assunto "Aedes aegypti"

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    Aproveitamento de resíduos de Chrysanthemum morifolium da Agroindústria Capixaba para produção de larvicidas naturais
    (Universidade Federal do Espírito Santo, 2021-03-01) Lima, Tamires Aliprandi; Kuster, Ricardo Machado; https://orcid.org/0000000289615348; http://lattes.cnpq.br/4149814906786366; https://orcid.org/0000-0001-5293-492X; http://lattes.cnpq.br/0339797150770837; Braga, Soraia Cristina Gonzaga Neves; https://orcid.org/0000-0001-9492-6629; http://lattes.cnpq.br/3912231591681676; Filgueiras, Paulo Roberto; https://orcid.org/0000000326171601; http://lattes.cnpq.br/1907915547207861; Romao, Wanderson; https://orcid.org/0000000222546683; http://lattes.cnpq.br/9121022613112821; Cunha Neto, Alvaro; https://orcid.org/0000-0002-1814-6214; http://lattes.cnpq.br/7448379486432052; Simas, Naomi Kato; https://orcid.org/0000-0002-9929-2714; http://lattes.cnpq.br/7460060045516567
    Chrysanthemum morifolium is a perennial species of the Asteraceae family. In Brazil its cultivation has as its main purpose to provide for the ornamental industry. In the agroindustrial process of the species, many residues are generated that do not present an adequate destination. The objective of the work was to determine the chemical profile of the extracts obtained from the residues of Chrysanthemum morifolium collected in the commercial establishments of the metropolitan region of Vitória - Espírito Santo and to verify if they present larvicidal activity for Aedes aegypti, allowing the formulation of a larvicidal product natural. To verify this hypothesis, 27 residues of Chrysanthemum morifolium were collected and extracted with solvents of different polarities, resulting in 27 dichloromethane (FA) and 27 methanolic (FB) extracts. In determining the chemical profile of the extracts, the analytical techniques adopted were: Fourier Transform Infrared Spectroscopy (FT-IR), application an unpublished for this species; Mass Spectrometry by Cyclotronic Ion Resonance by Fourier Transform (FTICR MS), with two different types of ionization source - Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI) in negative and positive modes respectively; and Gas chromatography coupled to the mass spectrometer (GC-MS). A mixture of the FA extracts and another of the FB extracts, at concentrations of 15 ppm were used for the initial tests of the larvicidal assays. The FB extracts showed no activity, in contrast 100% of activity was observed for the mixture of the FA extracts. Subsequently, larvicidal tests were carried out on the 27 FA extracts - at 5 and 15 ppm. For the concentration of 15 ppm, high larvicidal activity (mortality ≥ 80%) was observed in 14 samples, medium activity (80% < mortality ≤ 40%) in 4 samples and 9 samples performed low activity (mortality < 40%). The tests at 5 ppm, a high larvicidal activity was observed in 8 FA extracts, data are unpublished for this concentration in the species Chrysanthemum morifolium. The results were converted into matrices and used as input to perform the Principal Component Analysis (PCA). The PCA’s of the GC-MS, ESI (-) FT-ICR MS and FT-IR analyzes made it possible to infer the classes of insecticidal substances, attributed to the structures of triterpenes, fatty acids and nalkanes present in the FA extracts. The PCA with the data of APCI (+) FT-ICR MS, was not sufficient for the discrimination of the larvicidal activity as for the chemical composition of the extracts, the result was attributed to the complexity of the spectra. The results show that residues of Chrysanthemum morifolium generated chemically similar dichloromethane extracts, and the class of triterpenes and fatty acids were the classes of substances that most contributed to the discrimination of the samples. The data suggest that triterpenes are the plant's larvicidal molecules and that fatty acids act as synergistic agents.