Doutorado em Ciências Fisiológicas

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Navegando Doutorado em Ciências Fisiológicas por Assunto "8-prenilnaringenina"

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    Caracterização dos efeitos comportamentais e neuroprotetores do fitoestrógeno 8-prenilnaringenina em camundongos fêmeas ovariectomizadas
    (Universidade Federal do Espírito Santo, 2023-11-29) Silva, Igor Ferraz da; Rodrigues, Livia Carla de Melo; http://lattes.cnpq.br/2084216553746326; https://orcid.org/0000000199658678; http://lattes.cnpq.br/3194715486338521; Batitucci, Maria do Carmo Pimentel; http://lattes.cnpq.br/0010148251489155; Sartorio, Carmem Luiza; http://lattes.cnpq.br/1299417616233163; Santos, Roger Lyrio dos; http://lattes.cnpq.br/1122196233280741; Bellozi, Paula Maria Quaglio
    Background: Estrogens play a number of roles in maintaining brain homeostasis, executing behaviors, and neuroprotection. After menopause, many women complain of cognitive problems, in part due to the sudden drop in ovarian hormone synthesis. However, estrogen replacement therapy has many side effects. In this context, phytoestrogens are often investigated as possible adjuvants in the treatment of postmenopausal symptoms. 8-prenylnaringenin (8-PN) is one of the most potent phytoestrogens known to date. Despite its role as an estrogen receptor modulator and vast therapeutic potential, the effects of this molecule on the brain and behavior of rodents, especially females, have not yet been investigated. This study hypothesized that 8-PN exerts effects on cognition by preventing oxidative damage in the brain and promoting the expression of neurotrophins, such as BDNF. Methods: Female mice were divided into 7 groups and received acute treatments with LPS (1 mg/kg), 8PN (1mg/kg or 2 mg/kg), a combination of LPS and 8-PN doses, or a combination of both vehicle solutions. Animals had their recognition memory, spatial memory and social behavior assessed by behavioral protocols. Prefrontal cortex and hippocampus samples were collected and analyzed for levels of lipid peroxidation using TBARS assay, and BDNF protein expression using Western blot. Results: A single injection of 8-PN in both doses was able to attenuate behavioral impairments caused by LPS exposure in the recognition memory, spatial memory and social behavior tasks. 8-PN in both doses was able to protect the prefrontal cortex and hippocampus of female mice against lipid peroxidation and 8-PN 2 mg/kg promoted BDNF protein expression in the hippocampus. Conclusion: The data obtained in the present study suggest that 8-PN has potential neuroprotective effects in the brain of female mice by improving cognitive performance, reducing levels of oxidative damage and increasing BDNF expression. Further investigation is needed to demonstrate the exact mechanisms by which 8-PN exerts its effects in the brain.