Envolvimento do receptor ERa e da peroxidação lipídica nos prejuízos de memória de reconhecimento causados pelo poluente ambiental tributilestanho (TBT) em camundongos fêmeas
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Data
2019-04-10
Autores
Silva, Igor Ferraz da
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Universidade Federal do Espírito Santo
Resumo
Tributyltin (TBT) is a synthetic organotin compound used in several areas of agriculture and industry. Recent studies have demonstrated that TBT has effects on several organs and tissues in rodents and other animais, impacting their endocrine system due to its endocrine disrupting effects, causing abnormal estrogen levels. In this study, female mice were exposure to TBT in daily doses of 250ng/kg, 500 ng/kg and 750 ng/kg, and their recognition memory performance was tested using the novel object recognition test. We further assessed the estrous cyclicity, prefrontal cortex (PFC) and hippocampus estrogen receptor α (ERα) protein expression and oxidative stress. TBT impaired recognition memory in short and long term, in all doses. We found ERα receptor expression reduced in the hippocampus of the subjects that received the dose of 750 ng/kg, and decreased in the PFC of the subjects that received the dose of 500 ng/kg, when compared to Control group. TBT induced lipid peroxidation in the PFC in all doses, when compared to Control. It was also possible to observe alterations in the reproductive cycle of the animals, in which the groups treated with TBT had longer cycles. Our data suggest that TBT has the capacity of affecting the cognition of female mice. TBT can also modify the expression profile of ERα in different cerebral areas and promotes oxidative damage in PFC.
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Estrogen receptor , Hippocampus , Oxidative stress , Prefrontal cortex , Recognition memory , Tributyltin , Córtex pré-frontal , Estresse oxidativo , Estrogênio , Hipocampo , Memória de reconhecimento , Tributilestanho