Influência da exposição aguda à fumaça de cigarro sobre parâmetros bioquímicos gasométricos e desempenho físico em ratos
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Data
2014-04-04
Autores
Souza, Gisela Vicentini de
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Editor
Universidade Federal do Espírito Santo
Resumo
Introduction. The smoke causes the development of diseases, promotes increased
risk of cardiovascular diseases and can cause even sudden death. Smokers are
exposed to high carbon monoxide concentrations present in cigarette smoke,
promoting the reduction in oxygen in muscle tissues with the potential to cause loss
of aerobic capacity and hypoxia. Objective. To investigate the time course of blood
gas biochemical changes and assess whether the biochemical changes induced by
EFC are associated with physical performance of rats submitted to physical test of
swimming. Methodology. Male Wistar rats were divided into two groups: control (C,
n=8) and acutely exposed to cigarette smoke (EFC), divided in three groups Mo 1
(0-30min post EFC, n=11), Mo 2 (30 -60min post EFC, n=14) and Mo 3 (60-120min
post EFC, n=10), for eight consecutive days. All animals were weighed before and
after the trial period, and subjected to performance evaluation for swimming physical
test and hemogasometric analysis of arterial, blood samples were obtained by
catheterization of the carotid artery. Results. Found reduction in body weight of the
animals EFC compared to control animals (C=524.6± 12,1g; Mo 1=463.3±10,9g; Mo
2=482.8±9.2g; Mo 3=499.3±9,8g). The values of hemogasometric analysis is
presented with a significant increase for the concentration of carboxyhemoglobin
(C=0.0±0.0%; Mo 1=25.4±0.6%; Mo 2=13.6±0.5%; Mo 3=8.2± 0.7%), with statistical
difference between groups (p=0.001); For Methemoglobin (C=0.1±0.1%; Mo
1=1.9±0.3%; Mo 2=0.4±0.1%; Mo 3=0.3±0.1%) was no statistical difference between
Mo 1 group compared to the C group (p=0.001); The Oxygen Saturation (C=78.2 ±
1.5%; Mo 1=91.4±2.0%; Mo 2=87.1±1.8%; Mo 3=84.5±2.9%) had statistical
difference between Mo 1 and Mo 2 groups compared to C group (p=0.003); For
deoxy-hemoglobin (C=21.90±1.5%; Mo 1=6.33±1.5%; Mo 2=11.31±1.5% and Mo
3=15.25±2.4%) was reduced significant among Mo 1 and Mo 2 groups compared to
C group (p=0.001); For Lactate (C=1.71±0,2mmol/L; Mo 1=2.62±0,2mmol/L; Mo
2=1.79±0,1mmol/L; Mo 3=1.54±0,0mmol/L), a significant increase between Mo 1
group compared with C group (p=0.012). For the evaluation of performance in
physical exhaustion test in swimming, there was no statistical difference in the
animals time EFC compared to control animals (C=373.0±39,8s; Mo 1=273.0±15,9s;
Mo 2=298.5±23,0s; Mo 3=318.4±24,2s). We note the significant association EFC
animals (p=0.001, r-0.886) between higher value carboxyhemoglobin with shorter
blood collection. Conclusion. Acute exposure to cigarette smoke causes changes in
blood gas and biochemical parameters affect the transport of oxygen. In the model
acute exposure to cigarette smoke used in this work, we found impairment in
physical performance in EFC rats subjected to physical exhaustion test in swimming.
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Palavras-chave
Smoking , Performance Evaluation , Blood Gas Analysis , Hypoxia , Tabagismo , Avaliação de Desempenho , Gasometria , Hipóxia