Efeitos cardiovasculares da peçonha do peixe-escorpião (Scorpaena plumieri), estudos In vitro
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Data
2009-03-27
Autores
Gomes, Helena Lima
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Universidade Federal do Espírito Santo
Resumo
The scorpionfish Scorpaena plumieri is one of the most venomous fishes in the Atlantic Ocean. Its venoms cause severe injuries leading to local and systemic effects, such as intense pain, edema, necrosis and cardiovascular and respiratory disturbing. The aim of the present study was to investigate the cardiovascular activity of Scorpaena plumieri venom in vitro. The studies were performed on whole rat hearts using a “Langendorff” preparation for perfused isolated hearts. The crude venom (5 - 80 µg protein) produced significant dose-dependent positive inotropic (15% - 117%), lusitropic (16,9% - 161,4 %) and vasoconstriction responses (24%). N=8. Partial purification of a cardiovascular active fraction from the venom extract was achieved by gel filtration on a Sephacryl S-200 HR column. Samples of 1 to 7 µg protein of CF reproduced the cardiovascular effects induced by crude venom on isolated hearts. CF produced dose-dependent positive inotropic (4,8% - 36,0%), lusitropic (8,2% - 37%) and vasoconstriction responses (25%). N=7. Prazosin and propranolol significantly attenuated these responses. The coronary vasoconstriction response to CF was also attenuated by chemical endothelium denudation. Data presented suggests that Scorpaena plumieri venom acts, at least partially, via adrenergic receptors and so do the cardiovascular fraction.
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Scorpaena plumieri , Cardiovascular , Artérias coronárias , Receptores adrenérgicos