Caracterização dos efeitos da euphorbia tirucalli sobre a função cardíaca de ratos normotenso
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Data
2023-02-15
Autores
Barroso, Maria Eduarda de Souza
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Universidade Federal do Espírito Santo
Resumo
There are many reports of people about using medicinal plants, ignore the existence of real scientifically proven effectiveness and many others end up abandoning allopathic treatments in the hope of healing through these plants. This behavior has become very common among patients diagnosed with cancer who, in search of a cure. The aveloz (Euphorbia tirucalli) it is a plant of African origin that is popularly used for various diseases, including the treatment of cancer. This latex has already presented several therapeutic actions such as antioxidant, immunomodulatory and even anticancer activity in some cell colonies. Most of the studies, however, were carried out in vitro, with a lack of in vivo studies to certify its properties. The aim of the present work was to characterize the actions of Euphorbia tirucalli on the cardiovascular system of wistar rats. For this, wistar rats were divided into 2 groups: control treated with water and treated with E. tirucalli at a dose of 13,06mg/Kg for 15 days by gavage. The animals were evaluated for blood pressure by tail plethysmography and functional aspects of the cardiovascular system such as contractility and relaxation were analyzed by cardiac hemodynamics. Tissue parameters such as cardiac remodeling and pulmonary congestion were also analyzed. The investigation of cellular parameters included the analysis of oxidative stress markers in the DNA of blood cells, bone marrow cells and heart cells, as well as biomolecular analyzes of oxidative stress in plasma. The percentage of apoptosis and possible necrosis was evaluated in cardiac cells from animals in both groups. The animals showed no difference between their weights and blood pressure before and after the treatments. Cardiac remodeling and pulmonary congestion indices did not differ significantly between groups. In the hemodynamic evaluation, the treated animals did not show changes in intraventricular pressure and heart rate, but a significant increase in the duration of the cardiac cycle can be observed in animals treated with E. tirucalli. In the evaluation of ventricular contractility (dp/dtmax + ) there was a significant increase in animals treated with E. tirucalli and a decrease in TAU, with no changes between groups in the dp/dtmax -. In the biochemical analyzes, a significant increase in AOPP can be observed (15,86±0,8µmol/L control group; 30,48±2,4µmol/L E. tirucalli group.) and Myeloperoxidade (0,051±0,006UA control group; 0,111±0,016UA E. tirucalli group) with this difference not being maintained between groups in the TBARS analysis. During the evaluation of reactive species in the isolated cells, an increase in the marking of DHE and DCF in the blood of the animals treated with speed 15 days can be observed, a result that is not repeated in the isolated cells of the bone marrow and only DCF demonstrated to be increased in cells heart of rats treated with E. tirucalli. No significant difference was demonstrated between the parameters and viability, apoptosis and necrosis evaluated in cardiac cells. The results indicate a possible harmful action of E. tirucalli on parameters of contractility and relaxation of the cardiac muscle and that this phenomenon probably involves oxidative stress. This implies the need for further investigations to better elucidate their mechanisms of action on a healthy organism.
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Sistema cardiovascular , Estresse oxidativo , Euphorbia tirucalli