Análise do proteoma mitocondrial de células do fígado de camundongos apoE knockout tratados com sildenafil
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Data
2017-05-19
Autores
Menezes, Thiago Nunes de
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Universidade Federal do Espírito Santo
Resumo
The role of plasmatic lipids in the chronic disease may be associated with oxidative stress, which is characterized by redox imbalance. Proteins are molecules involved in almost all biological phenomena and the understanding of the protein regulation may be a source of new therapeutic strategies. Thus, the aim of the present work was analyse the mitochondrial protein expression in 18-weeks old sildenafil-treated apoE-/- mice as compared to non-treated animals. Previously, the apoE-/- mice presented a plasma lipid profile characteristic of dyslipidemia, and the triacylglycerol, total cholesterol, LDL and VLDL levels were 5, 14, 6 and 36 times higher than observed from control group (C57), which was not reverted by treatment with sildenafil. Flow cytometry showed in liver cells increased levels of intracellular reactive oxygen species of superoxide anion (~82%), hydrogen peroxide (~60%), peroxinitrite (~53%) as well as increased apoptotic cells (from ~2% to ~19%). The treatment was able to prevent the production of analysed ROS and reduced the apoptotic cells, restoring the pattern observed in C57 group. It suggests that sildenafil presents an antioxidant action. Differential two-dimensional electrophoresis coupled with mass spectrometry was applied to study the changes in mitochondrial protein profile in both conditions.
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Sildenafil , Oxidative stress , Mitochondrial proteome , Dislipidemia , Estresse oxidativo , Proteoma mitocondrial
Citação
MENEZES, Thiago Nunes de. Análise do proteoma mitocondrial de células do fígado de camundongos apoE knockout tratados com sildenafil. 2017. . Tese (Doutorado em Ciências Fisiológicas) - Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, 2017.