Avaliação do polimorfismo de receptores adrenérgicos na hiper-reatividade cardiovascular de testes estressores
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2011-06-17
Autores
Simões, Giovana Machado Souza
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Universidade Federal do Espírito Santo
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Background: In Brazil, the cardiovascular disease are the main cause of morbimortality, being responsible for 27,4% of the deaths caused for illnesses of cardiovascular origin (Health department, 2007; VI Brazilian Lines of direction of Arterial Hypertension, 2010). In recent study epidemiologist carried through in our city (Project MONICA) it was verified that 37% of the individuals in the band of 18 the 64 years of age were carrying of systemic arterial hypertension (Molina, 2002). The precocious detention of familiar antecedents for the cardiovascular disease allows the evaluation of its development as well as application of prophylactic measures, being late or until preventing the installation of injuries in agency-target. Therefore, our study it aims at to verify the association of the polymorphism of the beta2 and alpha1- adrenergics receptor with exaggerated the pressor and cardiac output reply during the laboratorial stress tests, in normotenses individuals. As well as, to correlate the hemodynamic responses with the sort and history of familiar antecedents of the stress tests Methods: The enlisted 98 volunteers of both (50mulheres/48 men), in the etária band of 20 the 55 years. Obeying the criteria of inclusion and authorization written for the TCLE, they had been gotten measured of the weight corporal (kg), height (m) and calculated the index of Corporal Mass (IMC=peso/altura2). Being excluded voluntary with cardiac disease associates, diabetes mellitus and obesity (IMC>30 Kg/m2). Collection of samples of peripheral venous blood of jejum of 8 hours was carried through, for evaluation biochemist (glicemia of jejum, total cholesterol and fractions, triglicérides) and for the extration of the DNA, in order to determine the polymorphism for analysis of restriction of polymorphism fragmentos (RFLP), extration of DNA, followed of amplification for Polymerase Chain Reaction (PCR) for polimorfos Arg16Gly, Gln27Glu and Arg492Cys, digestion of the amplified product, with genotipagem in the gel of acrilamida 10%. Carried through measured of accidental Pará, followed of the application of the test of Handgrip, color Stroop test and pressor Cold. As analysis statistics, test t of student for independent samples was used. In the not-parametric analysis of categorical 0 variable, these had been compared using the qui-square, including the test of comparison of ratio. The differences between two samples had been considered significant when p<0.05. The test of Hardy-Weinberg was carried through to get the alélicas frequencies and genotypc and to verify if the studied population it is in genetic balance. Followed of the analysis of the disequilibrium of linking, manual way and later confirmed through software Arlequin 3,11 (freeware) for analysis of data of population genetics.
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SIMOES, Giovana Machado Souza. Avaliação do polimorfismo de receptores adrenérgicos na hiper-reatividade cardiovascular de testes estressores. 2011. Tese (Doutorado em Ciências Fisiológicas) - Universidade Federal do Espírito Santo, Centro de Ciências da Saúde, Vitória, 2011.