Análise fitoquímica e avaliação das antioxidante, antimutagênica e citotóxica do estrato hidoalcoólico de Coriandrum sativum L.
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Data
2016-03-28
Autores
Santos, Patricia Carara dos
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Universidade Federal do Espírito Santo
Resumo
Coriandrum sativumL., known as coentro, is included in the Apiaceae family and It is a vegetable originating from European and African Continent. Flowers, leaves and fruits are commonly used of this plant. In addition it plays an important role in folk medicine and It is recommended for treatment of various diseases. This plant is rich in phenolic compounds that are related to Its antioxidant effects. The substrate involved in medicinal plant cultivation process can interfere in the production of secondary metabolites with bioactive principles . Another important factor in the production of metabolites is the development stage in which the plant is and there is no scientific works with this approach involvingC. sativum. The aims of this study was evaluate the influence of fertilization on the production of secondary metabolites in two stages of development of Coriandrum sativumand relate these conditions with quimioprotetor, antimutagenic and antioxidantpotential of hydroalcoholic extract of the leaves of thisplant. Theplants were grown in the Venda Nova do Imigrante/ES –Brazil under the organic fertilizer regimes (bovine manure) and chemical fertilizer (NPK), field conditions, and was evaluated two stages of development (vegetative and flowering). Aerial parts were dried and subjected to maceration in 70:30 ethanol/water (vol/vol) to obtain the crude extract which were underwent a phytochemical characterization by colorimetric methods and mass spectrometry. All cultivation conditions showed that the extracts had same phytochemical classes of metabolites (coumarins, flavonoids and steroids) andthe mass spectrometry indicated similarities between the extracts evaluated. The crude extract of C. sativumin the vegetative stage and chemical fertilization showed better antioxidant activity, according to the DPPH test when compared to other treatment groupsandthe extracts obtained from vegetative stage of the 8plantsunder different cultivation conditionsshowed a reduction in the micronucleus frequencyin relation to the positive control in the pretreatment and simultaneously assay.
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micronúcleo , adubação , mutagênese , quimioproteção