Avaliação da Biodistribuição de mercúrio em roedores após exposição crônica
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Data
2016-10-31
Autores
Gomes, Mariana Zanotelli
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Editor
Universidade Federal do Espírito Santo
Resumo
Mercury needs to be constantly monitoring due to of its toxic characteristics in concentrations at trace levels, leading to various disorders in the kidney, respiratory tract and in the central nervous system and diseases such as Alzheimer and Parkinson. The present study describes the mercury biodistribution in rat blood and organs (heart, brain, kidney, liver and testicle) after chronic administration of mercury chloride (HgCl2). The animals where divided into three experimental groups: 6 control (CT) treated with NaCl; 7 treated with 2.172 µg.kg-1 of HgCl2 (G1) and 7 treated with 21.72 µg.kg-1 of HgCl2 (G2). After 30 days, the rats were killed, and blood and organs were collected. For the microwave assisted decomposition, about 0.1 g of sample was weighed into a Teflon tube, to which were added: 1 mL 30% v/v H2O2; 6 mL of ultra-pure H2O and 1 mL of concentrated HNO3. For determination of mercury in the blood, 200 µL of blood was added in a 15 mL polypropylene tube and diluted with 0.005% w/ v Triton-X in 0.5% v/v 10 mL nitric acid (dilution was 1 + 49). The mercury was determined by inductively couples plasma mass spectrometry (ICP-MS), obtaining limit of detection (LOD) and limit of quantification (LOQ) of 6.0 ng.L-1 and 10.0 µg.kg -1 , respectively. Regarding the organs, for group G1, the kidney Hg concentration was 302.0 ± 45.1 µg.kg-1 , the remaining ones were below LOD. For the G2 group heart, brain and testicles being the organs with the lowest concentrations of mercury: 15.6 ± 4.6; 13.9 ± 2.0 and 15.8 ± 2.4 µg.kg -1 , respectively. The liver had a mercury concentration of 25.5 ± 7.7 µg.kg -1 and the kidney was the target organ, whose concentration was 2818 ± 1586 µg.kg -1 . The concentration of mercury found in blood in groups G1 and G2 were respectively: 3.8 ± 0.6 µg.kg -1 and 4.3 ± 0.5 µg.kg -1 . The results indicate that mercury is preferentially concentrated in the kidneys and liver and is not available in the blood.
Descrição
Palavras-chave
Biological sample , Mercury , Trace element , Amostra biológica , ICP-MS , Mercúrio , Elemento traço
Citação
GOMES, Mariana Zanotelli. Avaliação da Biodistribuição de mercúrio em roedores após exposição crônica. 2016. 44 f. Dissertação (Mestrado em Química) – Universidade Federal do Espírito Santo, Centro de Ciências Exatas e da Terra, Vitória, 2016.