Análise da junção cemento-esmalte utilizando-se da técnica da réplica e da microscopia eletrônica de varredura
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Data
2012-08-31
Autores
Escórcio, Bruna Pereira Silva
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Editor
Universidade Federal do Espírito Santo
Resumo
The cemento-enamel junction (CEJ) is the anatomical juncture between the enamel and cementum that covers the tooth crown and root, respectively. When changes expose the CEJ to the oral environment, the hard dental tissues of this region are vulnerable to pathological changes such as root caries, resorption, cervical erosion, abrasion and dentine hypersensitivity. The CEJ region was evaluated using the replica technique for SEM, in order to observe the different interrelationships among its mineralized tissues; if there is dentin permeability (gaps and defects with exposed dentinal tubules and lateral canals close to this region); to analyze the regions of CEJ with non-carious cervical lesions (NCCLs) and make a correlation between the clinical and ultrastructural findings in teeth with endodontic, periodontal and endo-periodontal infections. It was used 69 faces, 44 teeth extracted from 23 patients who were underwent clinical and radiographic examination, prior to extraction, aiming to obtain pulpal, periodontal and endo-periodontal clinical diagnosis. The teeth were cleaned and prepared, to make replicas made with the polyvinilsiloxane impression material and epoxy resin. The positive replicas were mounted on metal stubs, sputter-coated and evaluated by SEM. The results showed that the technique used was faithful and accurate on the reproduction of the ultrastructural details of the cervical region. The four types of morphological interrelationship among the hard tissues were found in the CEJ, in a irregularly and unpredictable distribution, because they showed from one to three of these interrelationships distributed on the same face, with a predominance of enamel overlapped by cementum (67.74%), followed by enamel and cementum edge-to-edge (56.45%), gaps between cementum and enamel (29.03%), and cementum overlapped by enamel (8.06%), which appears in few and small portions of the CEJ. There was statistical association between the interrelationship cementum over enamel and pulpal normal condition and there was also at cementum over enamel a tendency to be present in teeth without endo-periodontal involvement. The presence of exposed dentin tubules was statistically significant in the group with endo-periodontal lesions. The NCCLs also had statistically significant relationship in the group of teeth with severe periodontitis, with the predominance of saucer-shaped lesions in this group.
Descrição
Palavras-chave
Cemento-enamel junction , Scanning electron microscopy , Replica techniques , Endo-periodontal lesions , Non-carious cervical lesion , Junção cemento-esmalte , Técnicas de réplica , Lesão endo-periodontal , Lesão cervical não cariosa
Citação
ESCÓRCIO, Bruna Pereira Silva. Análise da junção cemento-esmalte utilizando-se da técnica da réplica e da microscopia eletrônica de varredura. 2012. 142 f. Dissertação (Mestrado em Clínica Odontológica) - Programa de Pós-Graduação em Clínica Odontológica, Universidade Federal do Espírito Santo, Vitória, 2012.