Mestrado em Ciências Farmacêuticas

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Navegando Mestrado em Ciências Farmacêuticas por Autor "Araújo, Mariana Ferreira Pereira de"

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    Efeito da intoxicação aguda pelo organofosforado triazofós sobre o processamento de respostas aversivas no medo condicionado ao contexto em ratos
    (Universidade Federal do Espírito Santo, 2020-08-21) Rodrigues, João Vitor Ferreira; Harres, Vanessa Beijamini; https://orcid.org/0000000345330495; http://lattes.cnpq.br/5077271160260796; https://lattes.cnpq.br/6812158906889133; Pires, Rita Gomes Wanderley; https://orcid.org/0000-0002-4739-8349; http://lattes.cnpq.br/8356031036198869; Araújo, Mariana Ferreira Pereira de; https://orcid.org/0000-0002-3016-8475; https://lattes.cnpq.br/8680061079519776
    Pre-clinical and clinical studies suggest that exposure to organophosphate compounds (OP) causes emotional and memory changes, possibly related to the inhibition of the enzyme acetylcholinesterase (AChE) in the central nervous system. Triazophos (TZP) is a moderate hazardous OP whose effect on aversive memory has not yet been investigated. Therefore, our objective was to assess whether acute TZP intoxication would impair aversive memory in the contextual fear conditioning (CFC). For this, adult Wistar male rats were submitted to the CFC (conditioning, extinction and test), in which the freezing response was measured. TZP was administered at doses of 7.5, 15 or 30 mg/kg (i.p) after the conditioning or the extinction session. At the end of each protocol, the animals were sacrificed for collecting of the hippocampus and amygdala, structures in which AChE activity was measured, as well as for collecting plasma to quantify butyrylcholinesterase (BChE) activity. In addition, independent cohorts of animals were treated with TZP to assess their influence on non-aversive associative memory in the novel object recognition test (NORT). The results showed that the dose of 15 mg/kg of TZP, administered immediately after the conditioning session of CFC, impaired the extinction of freezing response. The administration of TZP immediately after the extinction session did not alter the consolidation of the extinction compared to the control group. There was a decrease in AChE activity in the hippocampus 24 and 48 h after the administration of TZP (15 and 30 mg/kg), and a decrease in the amygdala 24 h after intoxication with the highest dose of TZP (30 mg/kg). Plasma BChE activity was reduced 24 or 48 h after TZP administration in all tested doses. The TZP did not change the discrimination of the new object in the NORT. Together, our results suggest that a single exposure to TZP impairs the acquisition/consolidation of the extinction in the CFC without affecting non-aversive memory. This effect seems to be related to AChE inhibition in the hippocampus of rats exposed to TZP.