Ciências Veterinárias
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Programa de Pós-Graduação em Ciências Veterinárias
Centro: CCAE
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URL do programa: http://www.cienciasveterinarias.ufes.br/pos-graduacao/PPGCV
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- ItemEfeito do protocolo de pré-sincronização em vacas mestiças no período pós-parto.(Universidade Federal do Espírito Santo, 2015-02-26) Almeida, Ítalo Câmara de; Oliveira, Fabrício Albani; Siqueira, Jeanne Broch; Barioni, GrazielaThis study aimed to evaluate the efficiency of the pre - synchronization hormonal protocol based in progesterone and estradiol in crossbred cows, milch , lactating , primiparous and multiparous , in early postpartum, regarding the follicular dynamics and reproductive efficiency . It were used 9 2 cows from 30 to 90 days postpartum, which were randomly allocated to two experimental groups, control ( CG ) and treatment ( TG ). In the control group , animals were subject only to time - fixed artificial insemination protocol ( TAI ), and TG animals were submi tted to a hormonal protocol for pre - synchronization before TAI . For the CG it were used 45 cows, in which 6 were used for analysis of follicular dynamics and 39 to evaluate the reproductive efficiency, and followed that up with the hormonal protocol: on da y zero (D0): insertion of intravaginal progesterone device (DIV P 4 ) new and intramuscular injection (IM) of 2 mg estradiol benzoate (EB); D8: removal of DIV P 4 IM , administration of 300 IU of equine chorionic gonadotropin (eCG) and IM administration of 0 , 15 mg of sodium cloprostenol (prostaglandin); D9: IM application of 1 mg of EB and; D10: TAI of the animals. For the TG it were used 47 cows, 8 of them for analysis of follicular dynamics and 39 to detect the pregnancy rate, and followed that up with the ho rmonal protocol of hormonal pre - synchronization: Day least nineteen (D - 19): inserting DIV P 4 previously used ( 4 th use) and IM administration of 2 mg of EB; D - 11: removal of DIV P 4 and IM application of 1 mg BE; elapsed 11 days from the withdrawal of DIV P 4 previously used, it began the protocol for T AI of animals with identical protocol to that used for the CG . The ultrasound exam s r egarding the follicular dynamics were performed on days 0, 8 and starting from the day of TAI (D10) in every 12 hours until t he determination of ovulation by the absence of the dominant follicle. After 30 days of TAI , it was estimated the pregnancy rate by ultrasound examination. Data were analyzed using SAS statistical program, considering the 5% critical probability. There was no significant difference (P ? 0 , 05) in the mean number of follicles on days 0, 8 and 10 between treatments. There was no significant difference (p ? 0 , 05) for follicular growth rate, which was 1 , 8 ± 0 , 24 and 0 , 79 ± 0 , 35 mm / day for the TG and CG groups, respectively. There was no effec t of treatment (P ? 0 , 05) to the diameter of the dominant follicle at the moment of T AI, and 12 , 5 ± 1 , 61 mm for the treatment group and 11 , 9 ± 2 , 26 mm for the control group. There were no effects of treatments (P ? 0.05) on the interval between the removal of proges terone device and ovulation of TG (65h25min ± 8h13min) and CG (66h ± 10h03min), and also of ovulation TAI, which was 14h1 5min ± 7 h 36min and 15 h 0 6min ± 9 h 0 4min for TG and CG groups, respectively. There were not effects of treatments (P ? 0.05) on the ra te of ovulation and pregnancy, which was 88 , 8% and 53 , 84%, respectively for the TG and 75% and 38 , 46%, respectively for the CG . There was a significant difference (P? 0.05) in pregnancy rate between primiparous cows (23.07%) and multiparous (69.23%) for the TG . It is concluded that the use of pre - synchronization protocol in crossbred cows in the early postpartum showed no marked differences in the evaluated patterns. Multiparous cows had higher pregnancy rate primiparous cows. The pregnancy rate of pre - synch ronization group of cows was satisfactory, above 50% for TAI protocols
- ItemInfluência do grau de sangue de doadoras Girolando na produção in vitro de embriões(Universidade Federal do Espírito Santo, 2021-02-26) Moulin, Gabriel do Nascimento; Carvalho Neto, José de Oliveira; https://orcid.org/; http://lattes.cnpq.br/3519418944409263; https://orcid.org/0000-0002-4161-766X; http://lattes.cnpq.br/0422351820916384; Bastos, Michele Ricieri; https://orcid.org/0000-0003-4611-9763; http://lattes.cnpq.br/4137177845564217; Almeida, Ítalo Câmara de; https://orcid.org/0000-0002-3349-1796; http://lattes.cnpq.br/4400814823088862The crossing between Bos taurus and Bos indicus, especially betweenthe Holstein x Gir breeds, are widely used in Brazil. However, information regarding the use of Girolando animals in an in vitro embryo production program (PIVE) is limited. The aim of this study was to evaluate the results of in vitro embryo production from donors Girolando ¼ (1/4 Holstein - ¾ Gir), ½ (½ Holstein - ½ Gir) and ¾ (¾ Holstein - ¼ Gir) donors, as well as analyzing the influence of the number of oocytes recovered, of the bull and the season on the PIVE indexes. Donors ½ (½ Holstein – ½ Gir) showed higher production of viable (19.7±0.6) and total (28.0±0.8) oocytes compared to ¼ (1/4 Holstein - ¾ Gir) oocytes 11.3 ± 1.0 viable and total 17.6 ± 1.4. Donors ½ were more efficient with 4.8 ± 0.2 produced compared to donors ¼ (2.6±0.3) and ¾ (1.6±0.3). Highest number of pregnancies (1.8±0.1) was found in donors ½ in relation to donors ¾ (0.6±0.2). Regarding the number of oocytes recovered, donors classified in the group of high oocyte production had a higher amount (28±3.0) and a percentage (72.4%) of viable oocytes compared to low production donors (13.3 ± 0.8; 68.9%), as well as a larger number of embryos produced (71.1±1.6 vs. 5.1±0.4). In the spring season, a higher percentage of oocytes was observed compared to winter (75% vs. 69,3%). Regarding the influence of the bull on the embryo and pregnancy rate, variations between 9.5 and 34.8% were found in the embryo rate, with no influence on the pregnancy rate. It is concluded that ½ donors are more efficient in embryo production than donors ¼ (1/4 Holstein - ¾ Gir), and ¾ (¾ Hostein - ¼ Gir). The winter negatively influences the percentage of oocytes recovered, and the bull has an influence on the percentage of embryos produced.