Ciências Veterinárias

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http://repositorio.ufes.br/handle/10/17470

Programa de Pós-Graduação em Ciências Veterinárias

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URL do programa: http://www.cienciasveterinarias.ufes.br/pos-graduacao/PPGCV

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Ação da própolis no perfil metabólico de ovelhas em fase reprodutiva
(Universidade Federal do Espírito Santo, 2013-11-28) Rangel, Charlene Candida; Cóser, Antonio Carlos; Stradiotti Júnior, Deolindo; Siqueira, Jeanne Broch; Silva, Veridiana Basoni
The aim of this research was to evaluate diets with and without inclusion of propolis ethanolic extract Verde Alecrim on dry matter intake, metabolic profile and its relationships of reproductive aspects and to compare metabolic profile values with reference values established on the scientific literature. Treatments were: Control (CT), control diet; Flushing (FT), control diet plus 300 g/animal/day of corn meal; Flushing with level 1 of propolis (FTP1), FT plus 8 mL/animal/day of propolis ethanolic extract; Flushing plus level 2 of propolis (FTP2), FT plus 12 mL/animal/day of propolis ethanolic extract. Sixteen Santa Inês nulliparous ewes, empty, four per treatment, were assigned in a complete randomized design, in a factorial scheme and subjected to an estrus synchronization protocol and covered. The females were housed and the experiment consisted of an adjustment period of ten days, followed by an experimental period of 30 days (15 days for pre-mating and 15 days for post mating). Animals were fed twice a day at 7 AM and 4 PM. To determine the hematobiochemical profile, blood samples were collected at each five days in fasting and two hours after during 15 days in the pre-mating phase and 15 days in the post mating to estimate urea, total protein, albumin, globulins and AST enzyme contents. Ultrasound to confirm pregnancy thirty days following mating was used. It was found that treatment with lower concentrations of propolis ethanol extract Verde Alecrim presented the potential to decrease DM intake intake and that the ethanol extract of propolis has proved effective in decreasing the dry matter intake and concentration of serum urea. The CT, FT and FP1T provided the same pregnancy rate, superior to TFP2, however FP1T resulted in higher rate of twin births. Treatments with a lower concentration of propolis reduced urea content in serum in fasting and two hours after feeding and in the post-mating phase. Most of metabolite contents were within reference values, according to the literature
Achados eletrocardiográficos e histopatológicos do miocárdio de ratos (rattus novergicus) wistar tratados com pimobendan
(Universidade Federal do Espírito Santo, 2015-07-14) Egert, Leandro; Aptekmann, Karina Preising; Nunes, L. C.
Aditivos não nutrientes na alimentação de codornas japonesas
(Universidade Federal do Espírito Santo, 2021-05-14) Gonçalves, Hortência César; Vargas Junior, José Geraldo de; https://orcid.org/0000-0001-6801-5629; http://lattes.cnpq.br/6468487749303265; https://orcid.org/0000000307839725; http://lattes.cnpq.br/9958062875924844; Silva, Eliane Aparecida da; https://orcid.org/0000-0002-0681-7797; http://lattes.cnpq.br/7204158118305449; Vieira, Danilo Vargas Goncalves; https://orcid.org/0000-0002-7407-9597; http://lattes.cnpq.br/2551239664072235
The Brazilian production of quail eggs shows remarkable growth. Knowing this, nutrition is having to adapt to this condition, providing nutrients for the animal's performance to be satisfactory. On the other hand, it is observed that there are restrictions on the use of additives used in rations, with the aim of improving and/or providing conditions for optimizing performance. Therefore, alternatives have been sought to replace the antibiotic additive used in the feed as a performance enhancer. Thus, the objective of this work was to evaluate the use of prebiotics, probiotics, symbiotics and organic acids in the productive performance of Japanese quails. The experiment was conducted in the fur farming sector of the Fluminense Federal Institute – Bom Jesus do Itabapoana-RJ Campus, where productive parameters and egg quality were evaluated. A total of 486 laying Japanese quails (Coturnix coturnix japonica) were used, with an initial average weight of 151.1g ± 27.18g for 84 days, divided into four phases of 21 days. A completely randomized experimental design was used, with six treatments basal ration (RB) + antibiotic, RB + probiotic, RB + prebiotic, RB + symbiotic, RB + organic acid 1 (100 g/ton ration), RB + organic acid 2 ( 200 g/ton of feed), nine replicates and nine birds per experimental unit. The variables analyzed were laying rate, feed intake, feed conversion, egg mass, average egg weight, dozen eggs, absolute and relative yolk weight, albumen and shell, yolk and albumen index and haugh unit. Data were submitted to the Student-Newman-Keuls (SNK) mean test at a significance level of 5% probability. Orthogonal contrast analysis was used, using the R software (Language and Programming). There was no statistical difference between the analyzed parameters. Under the experimental conditions of the present work, probiotic, prebiotic, symbiotic and organic acid additives can replace antibiotics as a performance enhancer.
Aferição do perfil metabólico em dois grupos genéticos de vacas primíparas holandês x gir em dois períodos da lactação no período da seca nos trópicos
(Universidade Federal do Espírito Santo, 2010-08-30) Bolzan, Raphael Pires; Stradiotti Júnior, Deolindo; Pereira Júnior, Olavo dos Santos; Clipes, Renata Cogo
Aferição do perfil metabólico em dois grupos genéticos de vacas primíparas holandês x gir em dois períodos da lactação no período da seca nos trópicos
Alterações fisiológicas e reprodutivas de Pseudosuccinea columella (Basomatophora: Lymnaeidae) frente a infecção experimental por Heterorhabditis bacteriophora hp88 (Rhabditida: Heterorhabditidae)
(Universidade Federal do Espírito Santo, 2022-02-21) Sperandio, Natania do Carmo; Martins, Isabella Vilhena Freire; https://orcid.org/0000000287003065; http://lattes.cnpq.br/6446528818006897; https://orcid.org/; http://lattes.cnpq.br/; Chambarelli, Melissa Carvalho Machado do Couto; https://orcid.org/; http://lattes.cnpq.br/; Alves, Vinicius Menezes Tunholi; https://orcid.org/; http://lattes.cnpq.br/; Alves, Victor Menezes Tunholi; https://orcid.org/; http://lattes.cnpq.br/
abstract
Alterações fisiológicas e reprodutivas em Pseudosuccinea columella (Mollusca: gastropoda) após infecção experimental por Heterorhabditis baujardi (Rhabditida: heterorhabditidae) cepa LPP7
(Universidade Federal do Espírito Santo, 2020-02-17) Vidal, Maria Larissa Bitencourt; Martins, Isabella Vilhena Freire; https://orcid.org/0000000287003065; http://lattes.cnpq.br/6446528818006897; https://orcid.org/0000000212605248; http://lattes.cnpq.br/; Ignacchiti, Mariana Drummond Costa; https://orcid.org/; http://lattes.cnpq.br/; Boeloni, Jankerle Neves; https://orcid.org/0000000300496854; http://lattes.cnpq.br/7669632202047727; Alves, Victor Menezes Tunholi; https://orcid.org/; http://lattes.cnpq.br/
Mollusc Pseudosuccinea columella participates in the prevalence of fasciolosis, leading to a distribution of the disease, as well as developing forms of control. This study attested under laboratory conditions the suceptibility of P. columella to Heterorh
Ambiente térmico e desempenho de leitões até a desmama alimentados com dieta suplementada com óleo de coco
(Universidade Federal do Espírito Santo, 2015-07-03) Deminicis, Renata Gomes da Silveira; Mendonça, Pedro Pierro; Vargas Junior, José Geraldo de; Paula, Marcos Oliveira de
Among the oils used for animal energy supplementation, coconut oil has been shown to be superior in weight gain and improved feed conversion. By having greater amount of medium-chain fatty acids makes has a different metabolic behavior by virtue of its structural characteristics, permitting high absorption in the intestine. The objective of this study was to perform environmental characterization, evaluate performance and mortality birth weaning piglets undergoing diet supplemented with coconut oil. The experiment was carried out using pig maternity facilities in White Clay Farm in the town of Canaan, located in the Zona da Mata, southeastern Minas Gerais. It is testing the use of coconut oil supplementation in suckling piglets from birth to weaning was done. 120 animals were distributed in a completely randomized design, with two treatments (supplementation with oil and without supplementation) with four replications (matrices) and 12 piglets each. The shelters provided stable conditions in the hottest hours of the day, over the stalls. The relative humidity remained below ideal levels for pigs at the hottest times of the day; BGT was appropriate on the recommendation of thermal comfort. It was found that there was no effect ( p> 0.05 ) of supplementation with coconut oil use over the average daily weight gain (g / day) of piglets until weaning , the obtained values were 227 g / day and 228 g/day , without the use and the use of coconut oil , however the mortality rate of the piglets was supplemented by 1.66 % for those who received no supplementation it was 5%, showing the importance of coconut oil when taking into consideration the production of piglets per sow .
Análise espacial da favorabilidade de ocorrência de cisticercose bovina no Estado do Espírito Santo
(Universidade Federal do Espírito Santo, 2013-07-30) Avelar, Barbara Rauta de; Santos, Alexandre Rosa dos; Martins, Isabella Vilhena Freire; Barioni, Graziela; Pinto, Paulo Sergio de Arruda
The bovine cysticercosis is a zoonotic parasitic disease with economic importance, caused by the larval stage of Taenia saginata,Cysticercus bovis. The objective of this work was to criate a risk map of the bovine cysticercosis occurrence in the Espírito Santo State . Through a literature review raised the following variables as important for the occurrence of there is parasitosis: sanitary sewage inappropriate (SSI), cattle population by municipality (CPM), use and occupation of the ground (UOG) and flood risk (FR). The database was criate using the data provided by IBGE for SSI and CPM. The data mapping was carried out in the program ArcGIS/ArcInfo 10.1., in which a mathematical model was develop involving the selected variables resulting map risk for the occurrence of bovine cysticercosis in the state. The map showed that the state of Espírito Santo predominate areas medium risk for the occurrence of the parasite, while areas of high and very high risk does not reach 12% of the study area and are mainly located in the northern region in the state. The use of GIS helped in the elucidation of the sites risk of bovine cysticercosis in the Espírito Santo State, and with it, more severe measures of control and prevention of bovine cysticercosis should be directed in the countries of the State with areas of high and very high risk
Análise espacial do risco de fasciolose bovina no estado do Espírito Santo por meio dos sistemas de informações geográficas
(Universidade Federal do Espírito Santo, 2013-02-27) Freitas, Deivid França; Martins, Isabella Vilhena Freire; Santos, Clóvis de Paula; Santos, Alexandre Rosa dos; Barioni, Graziela
The fasciolosis is a disease that affects domestic and wild ruminants and eventually the man. By causing great economic losses to cattle ranching and the high power of global dissemination, it sought alternatives to facilitate the understanding and visualization of disease delimiting potential risk areas in the Espírito Santo state. Thus, favorability maps were drawn on the current and future risk to F. hepatica based on climatic and environmental variables considered essential for maintenance of the disease and bioclimatological zoning maps and prevalence through the Geographic Information System (GIS). The data of cattle slaughter in the Espírito Santo were collected from the 11 slaughterhouses supervised by federal and state inspection services (SIF/SIE/MAPA), beyond the origins of livestock slaughtering in a slaughterhouse located in the municipality of Anchieta in the years 2009 to 2011. So, mapping results showed that areas of high risk gaskets totaled 35.42% for the current risk and tended to lessen with increases of 1°C to 5°C in temperature for 33.84%. The zoning map showed that 52.24% of the state areas are located in zones considered suitable for disease development. The combination of maps generated by zoning and prevalence of the 11 slaughterhouses distributed in the state showed that the highest prevalence rates were found in the municipalities located in the Southern region with averages ranging from 0.01% to 28.41%. Considering the result became clear that the epidemiological data, combined with GIS become a valuable tool for making decisions in order to minimize the occurrence and prioritize strategies for prevention and control of fasciolosis in Espirito Santo
Associação entre a leucose enzoótica bovina e a mastite
(Universidade Federal do Espírito Santo, 2017-02-23) Almeida, Sayanne Luns Hatum de; Zanini, Marcos Santos; Barioni, Graziela; Martins, Isabella Vilhena Freire; Donatele, Dirlei Molinari
Enzootic Bovine Leukosis (EBL) in addition to causing economic losses leads to important changes in immunity. This end up compromising the response aga inst other pathogens, thus increasing the chance of developing other concomitant disease s, mainly infectious diseases, among them mastitis. The objective of the presen t study was to evaluate whether there is an association between EBL and mastitis, in addition to determining the prevalence of EBL and mastitis in dairy cows in the Caparaó reg ion, south of Espírito Santo State. 899 crossbred dairy cows were used in d ifferent stages of lactation coming from properties located in the 12 municipalities th at compose the region of Caparaó Capixaba, in the south of Espírito Santo. The detection of clinical mastitis was performed by identifying the clinical signs of inflammation in the ma mmary gland and by the test of the black background mug and subclinical mastitis was diagnosed by the CMT (California Mastitis Test) test. The diagnosis of EBL wa s performed using the agar gel immunodiffusion technique (A GID ). The results were tabulated and the prevalence was demonstrated through descriptive a nalysis. Statistical analysis of Spearman's correlation coefficient was performed to ve rify the degree of correlation between presence of mastitis, clinical mastitis, presence of subclinical mastitis and EBL. Associations between dependent variables (mastitis, clinical mastitis and subclinical mastitis) and the independent variable ( EBL ) were estimated by the ratio of Odds Ratio (OR) and their 95% confidence intervals. The chi- square test was used to verify the significance of the associations. The prevalence for EB L found in Caparaó was 56.51% (508/899). Of the 70 properties, 94.29% h ad at least one positive animal for the disease. The presence of clinical and subclinica l mastitis was observed in 5.78% (53/899) and 44.27% (398/899) of the eva luated animals, respectively. A total of 61.24% (253/418) of cows with mastitis and positive for EB L were found. Mastitis ( ? = 0.088, p = 0.008) and subclinical mastitis ( ? = 0.091, p = 0.006) showed a significant positive correlation with leukosis at the 0. 01 level of significance. While clinical mastitis ( ? = 0.077, p = 0.021) showed a positive correlation with leucosis. The Odds Ratio (OR) ranged from 1,101 - 1,874, 1,099 - 3,74 5 and 1,109 - 1,893 for mastitis, clinical mastitis and subclinical mastitis respectively. That is, Bovine Leukosis virus (BLV) infection increases the chances of the infected animal acquiring mastitis, clinical or subclinical mastitis. It was observed in this study that animals with EB L are about 2.03% more likely to acquire clinical mastitis, and 1.45% more likely to acquire subclinical mastitis. In view of the data obtained, it is possible to conclude that BLV is widely distributed in the dairy herds of Caparaó Capixaba region and the prevalence of mastitis is high. In addition, it has been found that EB L may increase the animal's chances of acquiring mastitis, be it in its clinical or subclinical form.
Atividade antimicrobiana de fenóis naturais contra microrganismos isolados do trato genital de éguas
(Universidade Federal do Espírito Santo, 2022-01-11) Lorenzoni, Priscila de Oliveira; Queiroz, Vagner Tebaldi de; https://orcid.org/000000028170125X; http://lattes.cnpq.br/9963384168622230; https://orcid.org/0000000178206226; http://lattes.cnpq.br/; Neto, Jose de Oliveira Carvalho; https://orcid.org/; http://lattes.cnpq.br/3519418944409263; Mendes, Tiago Antonio de Oliveira; https://orcid.org/; http://lattes.cnpq.br/
Endometritis is one of the main causes of infertility in mares, generating economic losses in the equine reproduction sector. The difficulty in treating this disease is related to the emergence of resistant strains and the limited range of drugs available
Atividade secretória e histologia uterina de cadelas pré-púberes com ablação parcial do desenvolvimento das glândulas endometriais
(Universidade Federal do Espírito Santo, 2013-08-26) Martins, Bianca Barcelos; Luz, Marcelo Rezende; Neves, Mariana Machado; Siqueira, Jeanne Broch
The use of steroids with actions in the female reproductive system may affect the development and differentiation of the uterus, which can lead to abnormal development of endometrial glands, with decreased reproductive efficiency and even sterility. Thus, this methodology could be applied in the population control of dogs. However, in cases of partial inhibition of the development of the glands is not known effects of steroids on the functionality formed glands, as well as the possible occurrence of endometrial fibrosis. This study aimed to evaluate the secretory activity of the endometrial glands of prepubertal bitches undergoing a protocol for partial ablation of endometrial glands development. We used sixteen female mongrel bitches from different litters, distributed in the MPA group (n = 8), females that received injections of medroxyprogesterone acetate (10mg/kg/SC), every 3 weeks, from day 1 after birth until the age of six months, and C (n = 8), control females, untreated, which received applications of 0.9% NaCl solution. At 6 months of age, ovariohysterectomy was performed in all animals, and samples of uterine horns were collected and fixed for histology and histochemistry. To evaluate the intensity of uterine secretion (grades 1-4), the stains of periodic acid Schiff (PAS) and Alcian Blue (AB) pH 2.5 were used. For histological analysis we used Masson s trichrome and toluidine blue staining. Statistical analysis was performed by Kruskal-Wallis test, using the R software, with significance level of 5%. No dog in both groups exhibited cystic endometrial hyperplasia (HCE) and presence of content in the uterine lumen. For PAS only the grades 1 (absent) and 2 (mild) were observed in both groups, without difference between groups. However, the MPA showed greater intensity of uterine secretion (grade 2) in comparison to the C group (p <0.05). With respect to AB pH 2.5, in both groups we also found only the grades 1 (absent) and 2 (mild), without statistical difference between groups. There was no Masson s trichrome staining in the periglandular region in both groups. There was greater amount of mast cells present in the region of the myometrium in relation to the endometrium in both groups. It is concluded that prepubertal bitches with partial ablation of uterine adenogenesis present minimal uterine secretory activity, absence of uterine periglandular fibrosis and presence of larger amount of mast cells in the myometrium in relation to the endometrium
Autohemoterapia em ratos (Rattus norvegicus) : efeito sobre o nível do fator de necrose tumoral (TNF-a) e leucócitos
(Universidade Federal do Espírito Santo, 2013-07-09) Cáo, Mirleide de Araújo; Porfírio, Lenir Cardoso; Zanini, Marcos Santos; Ignacchiti, Mariana Drummond Costa
The autohemotherapy (AH), used for more than 30 years is an old procedure that in recent years, are among the studies that involve human and veterinary medicine. The AH stimulating the proposal of increasing macrophages in order to combat bacteria, viruses and cancer cells. For stimulation of the reticuloendothelial system, bone marrow produces more monocytes that will colonize the tissues and given the name macrophage. For this reason, the Tnf-alpha was quantumly tested to evaluate the defense system in rats that could be used or not, as an alternative to contribute to immunogenicity and protection levels in pets. Among the important cintocinas is the tumoral necrosis factor-alpha (TNF-a), which is secreted by macrophages, T cells, B cells and fibroblasts, and can act on almost all nucleated cells. It is also a mediator of many inflammatory and immune functions, which regulates the growth of many cell types. For the evaluation were used 16 healthy rats, Wistar, with the same weights and ages. The animals were divided into two groups with eight animals each, being the G1 the control group (physiological saline) and G2 the AH group. All animals had their blood collected for laboratory analysis before any procedure. Blood was collected for laboratory analysis to determine the levels of TNF-a and CBC. For quantitation of TNF-a was used ELISA kit (-linked immunosorbent assay) b100784 Rat TNF-a and the color intensity was measured at 450 nm. When evaluating this work the values of Tumor Necrosis Factor (TNF-a) present, as well as WBC of rats submitted to two AH procedures. The results show that there is increased TNF-a after application of HA and the number of lymphocytes and monocytes in the moment M2 also increases with a reduction in the production of cell M3. Based on the data obtained in this work, there were changes in the levels of TNF-a, the increase is evident 8 hours 7 days after the procedure AH. In the WBC is increased number of cells, such as lymphocytes and monocytes 8 hours 7 days after application of HA
Avaliação biomecânica, macroscópica e microestrutural de ossos longos de Cerdocyon thous (Linnaeus, 1766)
(Universidade Federal do Espírito Santo, 2020-02-18) Pastor, Felipe Martins; Silva, Maria Aparecida da; https://orcid.org/; http://lattes.cnpq.br/9360093834818219; https://orcid.org/0000000194475055; http://lattes.cnpq.br/; Boeloni, Jankerle Neves; https://orcid.org/0000000300496854; http://lattes.cnpq.br/7669632202047727; Franco, Guilherme Galhardo; https://orcid.org/; http://lattes.cnpq.br/2672607439191221
abstract
Avaliação cardíaca e metabólica de gatos obesos
(Universidade Federal do Espírito Santo, 2013-04-04) Borlini, Daniel Cometti; Aptekmann, Karina Preising; Costa, Fabiano Séllos; Champion, Tatiana
Avaliação cardiovascular de cães em treinamento de busca, resgate e salvamento.
(Universidade Federal do Espírito Santo, 2017-02-22) Santos, Paula Otoni Pereira Ronzani; Aptekmann, Karina Preising; Ferreira, Felipp da Silveira; Trivilin, Leonardo Oliveira
Rescue-trained dogs are used as aidin search operationsof living personsand/or corpses. Dogs perform intense physical activity during training sessions andoperations, that promotes a series of adaptive changes in the body. The term “athlete's heart” is used to describe cardiac morphological and functional changes that occur in athletes as a result of physical exercise, and physiological effects caused by exercise can resemble disease states. The aim of this study was to evaluate the cardiovascular responses caused by rescue training in dogs. Seven healthy dogs not practicing physical activity (sedentary group) were evaluated and compared to nine healthy dogspracticing search and rescue training (training group) at rest. Evaluations were also performed with the dogs of the training group before and after the search and rescue training. The animals were submitted to echocardiographic, electrocardiographic andsystemic systolic blood pressure (SBP) measurements.The data were statistically compared between the groups; as well as the comparison of the parameters evaluated in the different moments of the training group. The results demonstrate that the physical effort required by the training caused cardiac alterations in dogs, observed by the echocardiographic exam and not present in the electrocardiographic and SBP exams. As well as acute responses of the body through exercise that did not impair ventricular function nor cardiac arrhythmias after performing 20 minutes of physical exercise. Indicating a good physical conditioning of the dogs, making them apt to be used in real occurrences.These changes, similar to those described in human athletes, suggest that this type of training may cause "athlete's heart syndrome" in dogs.
Avaliação cardiovascular e índice glicêmico em estro, gestação e puerpério em cadelas
(Universidade Federal do Espírito Santo, 2015-02-27) Aguiar, Mayara Cristini Ferreira de; Aptekmann, Karina Preising; Champion, Tatiana; Favarato, Evandro Silva
Avaliação citopatológica, imunocitoquímica e teste do cometa do lavado vesical de bovinos com hematúria enzoótica
(Universidade Federal do Espírito Santo, 2013-07-31) Azevedo, Marcel Arcanjo Silva; Nunes, Louisiane de Carvalho; Barioni, Graziela; Carvalho, Eulogio Carlos Queiroz de
The bovine enzootic haematuria (BEH) is a chronic disease caused by Pteridium aquilinum and is characterized by the formation of non-neoplastic and neoplastic lesions in the bladder of the affected animals and has no specific treatment. In the southern region of the Espírito Santo, Brazil, this disease has a high prevalence and is responsible for major economic losses in dairy cattle, resulting from late diagnosis of the disease. Cytology associated with the use of biomarkers could aid in the early diagnosis of this disease. The aim of the study was to evaluate lesions bladders of cattle associated with enzootic hematuria through cytological techniques, immunocytochemistry and comet assay, for the diagnosis of early cellular damage caused by the disease. The study was divided into two experiments. In experiment 1, to standardize the harvesting technique, obtain samples and perform cytology of the bovine washing urinary bladder with BEH, were used 10 cattle, adult females divided into two groups. In group A was recovered all the liquid infused in the bladder, in group B, only the last liquid was recovered. In experiment 2, the cells obtained by bovine washing urinary bladder and were evaluated by immunocytochemistry using anti-p53 and comet assay and were used 10 cattle, adult females, divided in two groups of five animals, five healthy and five with BEH. The material was fixed and subjected to tests cytological, immunocytochemical and comet assay and evaluated microscopically. Data were analyzed using descriptive statistics, analysis of variance and nonparametric Kolmogorov-Smirnov test. Regarding the number of inflammatory cells and epithelial cells obtained per sample revealed that the two groups of experiment 1, all animals had more inflammatory cells than epithelial cells, however, there was no difference between the type of washing taken. The epithelial cells were found in 60% of cases and the alterations observed were discretes and cannot classify any sample as hyperplastic or neoplastic. In experiment 2, we observed positive immunostaining of p53 in only 20% of the samples, one of healthy animal and another animal with BEH. The comet assay revealed that both the samples of healthy animals as well as in animals positive for BEH not observed migrating nuclear fragments. Data from this study showed that the cytological examination of bovine washing urinary bladder may aid in the diagnosis of BEH and the two harvesting methods employed were adequate for obtaining viable samples. Cytology allowed the identification of non-neoplastic lesions predominantly inflammatory and immunocytochemistry technique with the expression of p53 as well as comet assay revealed no important cell damage since the animals used in the experiment had no neoplastic lesions
Avaliação de diluidores de congelamento de sêmen por meio de utilização de sondas fluorescentes em garanhões da raça mangalarga
(Universidade Federal do Espírito Santo, 2013-06-28) Costa, Márcio Nunes Cordeiro; Martins, Carla Braga; Barioni, Graziela; Aurnheimer, Rita de Cássia Martins
Avaliação de genitoras sexuais de Brachiaria ssp. Na época de seca.
(Universidade Federal do Espírito Santo, 2014-08-18) Pandolfi Filho, Antonio Delunardo; Deminicis, Bruno Borges; Chambela Neto, Alberto; Cóser, Antonio Carlos
Currently Brazil is the largest producer and exporter of tropical forage seeds in Latin America where demand for quality and quantity of seeds have been driving the entire production chain. This study aimed to evaluate sexual interspecific hybrid populations aiming at selecting genitors to assist in diversification of cultivated tropical pastures of genus Brachiaria. The experiment was conducted at Embrapa Beef Cattle in Campo Grande-MS, in the period January-August 2013. Progenies of half-brothers test was performed, for selection between and within families, and selected the best genitors on the basis of progeny and also the best individuals within progenies. The experimental design was a randomized block with ten replicates and five plants were individually for production total dry matter and leaf, percentage of leaf, leaf:stem ratio and sprouting capacity. Adopting the selection intensity of 1% of the selected hybrids, 100% of the products are of the best cross progenitor, with gains selection ranging from 11.07% to 30.39%. These results allow us to affirm that it is possible to make a selection of the best individuals with significant gains in the breeding program.

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