Autohemoterapia em ratos (Rattus norvegicus) : efeito sobre o nível do fator de necrose tumoral (TNF-a) e leucócitos
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Data
2013-07-09
Autores
Cáo, Mirleide de Araújo
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Universidade Federal do Espírito Santo
Resumo
The autohemotherapy (AH), used for more than 30 years is an old procedure that in recent years, are among the studies that involve human and veterinary medicine. The AH stimulating the proposal of increasing macrophages in order to combat bacteria, viruses and cancer cells. For stimulation of the reticuloendothelial system, bone marrow produces more monocytes that will colonize the tissues and given the name macrophage. For this reason, the Tnf-alpha was quantumly tested to evaluate the defense system in rats that could be used or not, as an alternative to contribute to immunogenicity and protection levels in pets. Among the important cintocinas is the tumoral necrosis factor-alpha (TNF-a), which is secreted by macrophages, T cells, B cells and fibroblasts, and can act on almost all nucleated cells. It is also a mediator of many inflammatory and immune functions, which regulates the growth of many cell types. For the evaluation were used 16 healthy rats, Wistar, with the same weights and ages. The animals were divided into two groups with eight animals each, being the G1 the control group (physiological saline) and G2 the AH group. All animals had their blood collected for laboratory analysis before any procedure. Blood was collected for laboratory analysis to determine the levels of TNF-a and CBC. For quantitation of TNF-a was used ELISA kit (-linked immunosorbent assay) b100784 Rat TNF-a and the color intensity was measured at 450 nm. When evaluating this work the values of Tumor Necrosis Factor (TNF-a) present, as well as WBC of rats submitted to two AH procedures. The results show that there is increased TNF-a after application of HA and the number of lymphocytes and monocytes in the moment M2 also increases with a reduction in the production of cell M3. Based on the data obtained in this work, there were changes in the levels of TNF-a, the increase is evident 8 hours 7 days after the procedure AH. In the WBC is increased number of cells, such as lymphocytes and monocytes 8 hours 7 days after application of HA
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Cytokine , Immune System , Pets , Animais , Citocina , Sistema Imune
Citação
CÁO, Mirleide de Araújo. Assessment of levels of tumor necrosis factor (TNF-a) and leucocyte count in rats (Rattus norvegicus) after procedure autohemotherapy. 2013. 40 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal do Espírito Santo, Alegre, 2013.