Mestrado em Ciências Veterinárias
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início: 2008
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo CNE (PORTARIA 609 de 14 de março de 2019) Publicado no D.O.U - 18/03/2019
Periodicidade de seleção: Semestral
Área(s) de concentração: Ciências Veterinárias
Url do curso: https://cienciasveterinarias.ufes.br/pt-br/pos-graduacao/PPGCV/detalhes-do-curso?id=1451
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- ItemEcobiometria esplênica em cães(Universidade Federal do Espírito Santo, 2025-10-03) Oliveira, Larissa Souza de; Aptekmann, Karina Preising ; https://orcid.org/; http://lattes.cnpq.br/; Cruz, Igor Cezar Kniphoff da ; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; 1º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 2º membro da banca; https://orcid.org/; http://lattes.cnpq.br/; 3º membro da banca; https://orcid.org/; http://lattes.cnpq.br/B-mode ultrasonography is a widely used imaging technique in small animal practice and serves as a fundamental tool for splenic evaluation, particularly in dogs. However, the lack of standardized parameters for spleen measurement hinders the diagnosis of splenomegaly as well as the differentiation between physiological and pathological conditions. In this context, the present study aimed to establish reference values for splenic size in healthy dogs of different sizes and ages, assessing distinct anatomical regions of the spleen using B-mode ultrasonography. Thirty healthy dogs were examined and allocated into three groups of ten animals each, according to body size, with a mean age of approximately three years and a mean body weight of 13 kg. The animals underwent physical and complementary evaluations, including complete blood count and abdominal ultrasound for measuring the height of the splenic head, body, and tail regions, as well as the diameter of the splenic hilum in longitudinal sec tion. Animals presenting systemic clinical, hematological, or ultrasonographic abnor malities were excluded from the study. The difference in dimensions among the various splenic regions was assessed between size groups, and within each group it was ver ified whether age range exerted any influence. The results demonstrated that spleen size varies according to the animals’ body size, being directly proportional to body weight. Within each group, the body of the spleen exhibited larger measurements com pared to other regions, whereas the tail and head showed no significant differences between them. Additionally, the splenic hilum showed variation according to dog size. No significant influence of age range on splenic dimensions was observed within groups. We conclude that body weight tends to influence spleen size and that B-mode ultrasonography is a safe and effective method for its measurement, contributing to the standardization of splenic assessment in this species
- ItemIsolamento, cultura e efeito de Viscum album L., doxorrubicina e associação na morfologia e expressão proteica de linhagem celular primária de adenomioepitelioma maligno canino(Universidade Federal do Espírito Santo, 2025-04-25) Angelos, Tamires de Almeida; Archanjo, Anderson Barros; https://orcid.org/0000-0002-9305-271X; http://lattes.cnpq.br/5529149503714764; Trivilin, Leonardo Oliveira; https://orcid.org/0000-0002-3039-3269; http://lattes.cnpq.br/5723897284006298; https://orcid.org/0009-0001-6359-5115; http://lattes.cnpq.br/7375975428092053 ; Boeloni, Jankerle Neves; https://orcid.org/0000-0003-0049-6854; http://lattes.cnpq.br/7669632202047727; Oliveira, Mayara Mota de; https://orcid.org/0000-0003-2735-9788; http://lattes.cnpq.br/5495329160438980Breast cancer is the disease that most affects female dogs in veterinary medicine, lacking precise therapies that affect only cancer cells, helping in tumor eradication and survival. Thus, the objective of this study was to isolate and cultivate a primary canine malignant adenomyoepithelioma (AME) cell line, and to evaluate the individual effects and the association of Viscum album L. (D3 dilution) and doxorubicin on the morphology and expression of BAX, BCL-XL, OGG, GPX and REF1 of a primary canine malignant adenomyoepithelioma cell line. A canine malignant adenomyoepithelioma sample from a 9-year-old SRD bitch was used for isolation and primary culture of cancer cells. Previously, the working LC50 of Viscum album L. (D3 dilution) was established in 24 and 48h (37.07 μL/mL), as well as the working concentration of doxorubicin, also after 24 and 48h of viability assay, defining the concentration 1.56 μg/mL, using cancer cells in primary culture, 20th pass. The concentrations determined were used for cell morphology and protein expression assays by immunohistochemistry after exposure for 24 and 48 hours to the treatments Viscum album L., doxorubicin and Viscum album L + doxorubicin association. The morphological findings showed minimal morphological differences compared to the control group, acting mainly as a cytoprotective agent, while doxorubicin alone and in association strongly induced cell death. The weak positive expression score maintained at 24 hours in the BAX, BCL-XL and GPX proteins in the Viscum album L. and doxorubicin groups compared to the control group, differing only from the associated treatment Viscum album L. + doxorubicin. At 48 hours, the weak positive score remained present in all treatments compared to the control group in GPX and BAX, differing only in BCL-XL the Viscum album L. and doxorubicin groups compared to the control and association group that presented a positive score. When comparing the times, it was obtained that doxorubicin increases the expression of GPX and BAX in addition to decreasing BCL-XL in 48 hours while the association acts by increasing the pro apoptotic protein. Viscum album L. decreased the nuclear labeling of OGG, while doxorubicin increased OGG and decreased the labeling of REF-1. The association of the compounds led to an increase in OGG and a decrease in REF-1. It is mainly concluded that the association of Viscum album L. and doxorubicin potentiate their mechanisms of action in a time-response manner, in addition to the fact that in SMA, isolated treatments induce the activation of other types of programmed cell death, in addition to apoptosis.
- ItemSoroprevalência e fatores de risco do coronavírus felino (FCoV) no sul capixaba(Universidade Federal do Espírito Santo, 2025-03-25) Caprioli, Ana Amélia; Delatorre, Edson Oliveira; https://orcid.org/0000-0002-5746-0820; http://lattes.cnpq.br/9814839314541002; https://orcid.org/0009-0000-1875-5303; http://lattes.cnpq.br/2835032810980724; Guerson, Yuri Barbosa; https://orcid.org/0000-0001-7796-0921; http://lattes.cnpq.br/6290312600632479; Archanjo, Anderson Barros; https://orcid.org/0000-0002-9305-271X; http://lattes.cnpq.br/5529149503714764Coronaviruses (CoVs) are zoonotic viruses belonging to the Coronaviridae family, capable of infecting various species, including felines. Feline Coronavirus (FCoV) can evolve into feline infectious peritonitis (FIP), a severe and often fatal disease. This study aimed to investigate the seroprevalence of FCoV in domestic cats from the municipalities of Alegre and Jerônimo Monteiro, in southern Espírito Santo, and to identify the main risk factors associated with infection. Blood samples were collected from 120 cats, aged between 41 days and 13 years, treated at the Patinhas Alegres veterinary clinic. Antibody detection was performed using the ImmunoComb FCoV test, an ELISA-based immunoassay that quantifies serological levels into categories of negative, weak, moderate, and strong positive. The seropositivity rate was 69.2%, with a distribution of 5.8% weak positive, 29.2% moderate positive, and 34.2% strong positive. Univariate analysis indicated a significant association between FCoV infection and age (p = 6.18 × 10⁻⁷) and reproductive status (p = 3.09 × 10⁻⁸), while sex (p = 0.4035) and housing conditions (p = 0.2628) were not statistically significant. Binary logistic regression confirmed that cats aged 3–8 years had an 11-fold higher risk of infection (OR = 10.96; 95% CI: 2.56 – 56.57; p = 0.002), and cats older than 8 years had a 9-fold increased risk (OR = 9.27; 95% CI: 1.42 – 86.91; p = 0.029), suggesting that cumulative exposure to the virus over time is a key determinant of infection. Ordinal logistic regression revealed that older cats not only had higher seropositivity rates but also exhibited elevated antibody levels. Compared to kittens (<1 year), cats aged 3–8 years were 8.8 times more likely to have high antibody titers (OR = 8.84; 95% CI: 2.45 – 34.94; p = 0.0012), while cats older than 8 years had a 19-fold increased risk (OR = 19.12; 95% CI: 4.11 – 100.37; p = 0.0003). Housing conditions showed a statistical trend, with cats living alone exhibiting a higher infection risk (OR = 9.48; 95% CI: 1.08 – 216.87; p = 0.041). However, this factor did not significantly influence antibody levels, suggesting that social contact may affect viral transmission but not necessarily the immune response. Given these findings, controlling FCoV infection in Brazil requires not only large-scale and continuous epidemiological studies to monitor high-risk groups, such as adult and elderly cats, but also the implementation of integrated biosecurity measures, including proper environmental management, targeted vaccination protocols, and increased awareness among cat owners to prevent viral spread and minimize the risk of progression to FIP.
- ItemFarinha de mosca soldado negro (Hermetia illucens), na nutrição do lambari (Astyanax bimaculatus)(Universidade Federal do Espírito Santo, 2024-09-27) Souza, Erivelto Oliveira de; Bolzan, Raphael Pires; https://orcid.org/0000-0002-3269-5180; http://lattes.cnpq.br/7880129709674385; Mendonça, Pedro Pierro; https://orcid.org/0000-0001-5128-0020; http://lattes.cnpq.br/3992381145892105; https://orcid.org/0000-0001-7212-1651; http://lattes.cnpq.br/6216330251223509; Vargas Junior, José Geraldo de; https://orcid.org/0000-0001-6801-5629; http://lattes.cnpq.br/6468487749303265; Gonçalves Junior, Lucas Pedro; https://orcid.org/0009-0000-2232-8443; http://lattes.cnpq.br/6111474381561776The growing global aquaculture production is increasing the demand for feed, with fishmeal being a notable but expensive component. Sustainable alternatives, such as black soldier fly (Hermetia illucens) meal, are being explored due to their efficiency and nutritional profile. This study nutritionally characterized black soldier fly meal in its various life stages and evaluated its effectiveness as an ingredient in diets for juvenile yellow tail-lambari (Astyanax bimaculatus). The nutritional characterization of the samples determined moisture, lipids, ash, crude protein, and gross energy content of the black soldier fly meals. The analyses were conducted in laboratories at IFES and UFES. Data were subjected to ANOVA and Tukey's test to check for significant differences. In performance experiments, the experimental feeds were formulated with levels of (T1 = control, T2 = 12.5%, T3 = 25%, T4 = 37.5%, and T5 = 50%) of black soldier fly meal from the pupal and adult stages. The fish were distributed through a completely randomized design, with 5 treatments and 4 replications, containing 10 fish per experimental unit, and the trial lasted 45 days. Statistical data were analyzed using ANOVA and polynomial regression. According to the nutritional characterization data, it can be deduced that the larval and pupal stages are the most suitable for use. The adult stage is less ideal due to the high chitin content. Performance data showed that diets replacing fishmeal with pupal black soldier fly meal yielded good results, indicating that this stage is usable. Conversely, diets containing adult black soldier fly meal did not perform well, likely due to excessive quitin.
- ItemEfeitos sedativos e hemodinâmicos da administração intranasal e intravenosa da nalbufina, buprenorfina e acepromazina em cães(Universidade Federal do Espírito Santo, 2025-02-24) Oliveira, Paula da Rocha; Barbosa, Breno Curty ; https://orcid.org/0000-0003-4171-7000; http://lattes.cnpq.br/4063131399526035; Trivilin, Leonardo Oliveira ; https://orcid.org/0000-0002-3039-3269; http://lattes.cnpq.br/5723897284006298; https://orcid.org/0009-0009-5166-509X; http://lattes.cnpq.br/2193248116733082; Cruz, Igor Cezar Kniphoff da ; https://orcid.org/0000-0002-0615-9990; http://lattes.cnpq.br/9546223095972612; Coelho, Nathália das Graças Dorneles ; https://orcid.org/0000-0002-2542-0103; http://lattes.cnpq.br/1406240305764857The aim of this study was to evaluate and compare the sedative effect and hemodynamic changes produced by intranasal (IN) and intravenous (IV) administration of nalbuphine (NALB) and buprenorphine (BUPRE), used alone or associated with acepromazine (ACP) in dogs. Seven healthy dogs (6 females and 1 male) with an average weight of 16.57 kg (±4.7), average age of 4.5 years (±1.05) were included and submitted to a before-and-after clinical trial, where each animal was its own control, with a seven-day washout between one administration and another of the experimental protocols. Each animal received intranasally: ACP 0.1 mg/kg; NALB 0.75 mg/kg; BUPRE 20 µg/kg; NALB 0.75 mg/kg + ACP 0.02 mg/kg; BUPRE 10 µg/Kg + ACP 0.02 mg/Kg; while intravenously: ACP 0.02 mg/Kg; NALB 0.5 mg/Kg; BUPRE 20 µg/Kg; NALB 0.5 mg/Kg + ACP 0.02 mg/Kg; BUPRE 10 µg/Kg + ACP 0.02 mg/Kg. Heart rate (HR) and respiratory rate (f), systolic blood pressure (SBP), rectal temperature (RT), blood glucose, degree of sedation and electrocardiogram (ECG) were evaluated before drug administration (baseline) and after at different time intervals (T0), (T05), (T15), (T25), (T35), (T45) and (T60). In relation to baseline, after IN administration, significant changes were: increased HR at T0 and T05 with ACP, reduced at T35 with NALB and at T45 and T60 with NALB+ACP; reduced SBP from T15 to T60 with ACP, from T05 to T60 with BUPRE and from T25 to T60 with BUPRE+ACP; reduced f from T25 to T60 with ACP and reduced at T35 with BUPRE; reduced TR from T35 to T60 in all protocols; increased blood glucose at T60 with NALB and BUPRE+ACP. After IV administration, significant changes in relation to baseline were: reduced HR and SBP at a given evaluation time with all protocols used; increased f from T05 to T45 with BUPRE and at T05 with NALB; increased blood glucose at T60 with ACP and BUPRE+ACP. Moderate sedation was observed from T05 until T60 for all protocols and routes used. By the IN route, sedation by ACP stood out significantly in relation to NALB and BUPRE, while these isolated drugs produced greater sedation in relation to their respective combinations with ACP. By the IV route, ACP promoted greater sedation than BUPRE at T15 and T25, while the combinations stood out significantly in relation to the isolated drugs. Tachypnea, vocalization and sialorrhea were observed in the BUPRE and BUPRE+ACP protocols (IN and IV routes), nasal secretion and salivation with NALB (IN and IV routes), and myoclonus with ACP (IN and IV routes) and BUPRE IN. With the use of BUPRE (IN and IV routes), loose stools and loss of appetite were observed afterwards. The ECG results indicated an increase after IN administration of BUPRE and ACP alone in the duration of the P and T waves, respectively, when compared to the IV route. The BUPRE protocol in the duration of the T wave and QT interval was superior in the IV route; BUPRE+ACP observed an increase in the PR and QT intervals. NALB+ACP showed an increase in the duration of the QT interval by the IV route in relation to the IN route. The two groups (IN and IV) presented a similar prevalence of arrhythmias (90%), with sinus bradyarrhythmia being the most frequent, and no incidence of atrioventricular blocks was observed in this study. It was generally concluded that isolated buprenorphine presented greater adverse and physiological effects, but its combination resulted in similar sedation in both routes with satisfactory sedative results and with few physiological effects observed. Regarding the electrical activity of the heart, administration by the IN route presented more stable values in the cardiac variables when compared to the IV route, demonstrating to be an alternative with great potential in the veterinary routine