Mestrado em Ciências Veterinárias
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início: 2008
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo CNE (PORTARIA 609 de 14 de março de 2019) Publicado no D.O.U - 18/03/2019
Periodicidade de seleção: Semestral
Área(s) de concentração: Ciências Veterinárias
Url do curso: https://cienciasveterinarias.ufes.br/pt-br/pos-graduacao/PPGCV/detalhes-do-curso?id=1451
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- ItemFauna malacológica dulcícola e identificação de larvas de trematódeos em região endêmica para fasciolose no sul do Espírito Santo(Universidade Federal do Espírito Santo, 2024-02-19) Pereira, Poliana Demuner; Martins, Isabella Vilhena Freire ; https://orcid.org/0000-0002-8700-3065; http://lattes.cnpq.br/6446528818006897; https://orcid.org/0000-0002-9320-7603; http://lattes.cnpq.br/3435589395448060; Pinto, Hudson Alves; https://orcid.org/0000-0002-5519-1348; http://lattes.cnpq.br/4751699050350958; Boeloni, Jankerle Neves ; https://orcid.org/0000-0003-0049-6854; http://lattes.cnpq.br/7669632202047727The digenetic trematode Fasciola hepatica is a parasite with the potential to cause significant losses in ruminant herds, also presenting a high zoonotic potential with aquatic snails being intermedial hosts. This study aimed to recognize the malacological fauna and identify trematodes larval types in endemic areas for fasciolosis in the southern region of Espírito Santo, as well as to correlate determining factors for the occurrence of the disease. The analysis of properties with a history of fasciolosis was conducted by collecting data from the Parasitology Laboratory of UFES, covering the period from 2020 to 2022. A visit was made to 10 properties, where all aquatic snails present were collected and identified. For the recovery of cercariae in the laboratory, snails were tested every 10 days for 4 hours using photo-stimulation. The identification of cercariae was based on morphological characteristics, and there was also histological processing of positive snails for the visualization of other larval forms. Ten properties were identified as positive for the presence of F. hepatica. The species of snails found in the region were P. columella, B. glabrata, M. tuberculata, and S. marmorata. The types of cercariae emerging from the snails were xiphidiocercariae and echinostomes, with no cercariae of Fasciola found. During histological analysis, a large number of sporocysts were observed in the tissues of P. columella individuals, as well as internal structures similar to cercariae in the vesicles. In the multivariate analysis, it was found that the factors that most influenced the results were the number of examinations carried out on the properties and the number of animals diagnosed as positive for fasciolosis. The data indicate that geographic distribution, property characteristics, and the presence of snails play a significant role in the dissemination of cercariae in the hydrographic network
- ItemAnálise espaço-temporal de fasciolose em ruminantes no estado do Espírito Santo(Universidade Federal do Espírito Santo, 2025-02-21) Pinheiro, Pedro Clemente Pereira; Martins, Isabella Vilhena Freire; https://orcid.org/0000-0002-8700-3065; http://lattes.cnpq.br/6446528818006897; Donatele, Dirlei Molinari; Carneiro, Milena BatistaFasciolosis is caused by the fluke Fasciola hepatica, which has a worldwide distribution and affects the bile ducts and liver parenchyma of several species of mammals, especially ruminants, and humans, configuring the disease as a zoonosis and generating a risk to global public health. In Brazil, the disease is considered endemic to some regions, mainly the South and Southeast, with emphasis on high rates in ruminants in the state of Espírito Santo. In this sense, the present study aimed to carry out a spatio-temporal analysis of fasciolosis in ruminants in the state of Espírito Santo, from 2018 to 2023, based on slaughter maps carried out by the State Inspection Service (SIE) and Federal Inspection Service (SIF). The data used to carry it out were obtained from nosographic maps referring to slaughterhouses located in municipalities in the state. For statistics, linear regression analysis was carried out regarding the slaughterhouses submitted to the SIE. To carry out the spatial distribution of notifications of fasciolosis in ruminants for each refrigerated slaughterhouse applied, they were represented in the preparation of maps by the QGIS 3.28.1 program, using data from geographic bases made available by the Brazilian Institute of Geography and Statistics. The average annual frequencies of bovine fasciolosis for animals slaughtered under SIF for the years 2021, 2022, and 2023 were 0.5%, 0.9%, and 0.93%, respectively, with an average frequency of 0.829%. For slaughterhouses under the responsibility of SIE, 36,856 cattle were slaughtered between 2018 and 2023, representing a frequency of 5.53% of fasciolosis. Considering the animals slaughtered for both SIF and SIE, between 2018 and 2023, of the 887,399 cattle slaughtered, 38,689 had a positive diagnosis for the disease, representing a frequency of 4.35%. In an analysis from 2006 to 2023, a slaughterhouse in the South region presented an average frequency of 19.65% of fasciolosis in cattle. High rates of the disease were recorded for buffalo slaughter, reaching frequencies of 66.66% and 59.64%, for the years 2022 and 2023, respectively, in a slaughterhouse in the south of the state, which also occurred with sheep in 2023, with a frequency of 18.18%. There was no slaughter of goats during the study period. The study concluded that the state of Espírito Santo demonstrates a high frequency of fasciolosis in ruminants, with the South region standing out with the highest frequency rates in slaughtered cattle, buffaloes and sheep, reinforcing the need for control measures for the disease in the region, minimizing the risks to public health as a consequence
- ItemPrincipais lesões anatomopatológicas em órgãos de bovinos condenados em matadouro frigorífico no sul do estado do Espírito Santo(Universidade Federal do Espírito Santo, 2023-04-25) Assis, Amanda Azevedo; Boeloni, Jankerle Neves; https://orcid.org/0000000300496854; http://lattes.cnpq.br/7669632202047727; https://orcid.org/0000000244393075; http://lattes.cnpq.br/5867453023309582; Martins, Isabella Vilhena Freire; https://orcid.org/0000000287003065; http://lattes.cnpq.br/6446528818006897; Baeta, Silvia de Araujo Franca; https://orcid.org/0000-0002-5654-6403; http://lattes.cnpq.br/0801139950064457The objective of the present study was to identify the main anatomopathological lesions in organs of condemned cattle during the post mortem inspection in a slaughterhouse in the south of the state of Espírito Santo, from March to June 2022. A total of 496 samples were collected from 1512 slaughtered animals, with the liver being the organ that presented the highest number of lesions. The main cause of condemnation was fasciolosis with 49.80% (n=247), followed by tuberculosis with 18.75% (n=93), telangiectasia in 16.33% (n=81), renal cyst in 8.06 % (n=40), liver abscess in 3.63% (n=18) and cysticercosis in 3.43% (n=17). Macroscopically, in cases of fascioliasis, white areas predominated, sometimes firm, sometimes creaking when cut in 63 (25.50%) livers; in cases of tuberculosis, multifocal granulomas predominated in lymph nodes (33/36; 91.66%), lungs (15/20; 75.00%), and liver (5/17; 29.41%); in cases of telangiectasia, the lesions were red to blackish areas in 60 (74.07%) of the livers; in the cases of renal cysts, the kidneys presented multifocal cysts in 40 (100%) and pallor in 32 (80.00%); in cases of liver abscesses, 100% (n=18) of the livers had this lesion; and in the cases of cysticercosis, viable cysts were observed in the masseter muscle (7; 41.17%) and tongue (1; 5.88%) and calcified cysts in the heart, kidney, liver and lymph node, all with 5.88%. The cases of fascioliasis, telangiectasia, renal cyst, liver abscess and cysticercosis, observed on macroscopy, were confirmed on microscopy; cases of tuberculosis observed in lymph nodes, lungs and liver on macroscopy were also confirmed on microscopy, however, cases on kidneys and spleen were not confirmed on microscopy. It was concluded that the main cause of organ condemnation was fasciolosis, followed by tuberculosis, telangiectasia, renal cyst, liver abscess and cysticercosis; the most condemned organ was the liver, with a predominant macroscopic lesion of white areas, sometimes firm, sometimes creaky when cut, and the most frequent microscopic alteration was degeneration of hepatocytes.
- ItemEfeitos fisiológicos e antinociceptivos da administração epidural de morfina e fentanil em equinos conscientes(Universidade Federal do Espírito Santo, 2023-02-17) Damaceno, Andressa Brito; Trivilin, Leonardo Oliveira; https://orcid.org/0000000230393269; http://lattes.cnpq.br/5723897284006298; https://orcid.org/0000000167197301; http://lattes.cnpq.br/0646128260805411; Rego, Rafael Otaviano do; https://orcid.org/0000-0001-9291-7219; http://lattes.cnpq.br/7032024290799824; Silva, Maria Aparecida da; https://orcid.org/0000-0003-0967-3925; http://lattes.cnpq.br/9360093834818219; Silva, José Ricardo Barboza; https://orcid.org/0000-0002-5027-2384; http://lattes.cnpq.br/1051905066193981Epidural analgesia with opioids in horses has excellent antinociceptive action, but is not widely used in hypiatrics due to their adverse effects. The objective was to compare the physiological and antinociceptive effects of morphine and fentanyl, isolated or co-administered, by epidural injection in conscious horses. Five healthy horses, submitted to epidural catheterization, were used. Each animal received morphine (0.1 mg/Kg), fentanyl (2 μg/Kg) and a combination of morphine (0.15 mg/Kg) and fentanyl (1 μg/Kg). The parameters evaluated at T0 (baseline) and at 15` (T025), 1h (T1), 4h (T4), 12h (T12) and 20h (T20) were: heart rate (HR) and respiratory rate (RR), intestinal motility, rectal temperature (RT), sedation, behavior, ataxia and antinociception scores against thermal (cold and hot) and mechanical stimuli, in ischial and coxal tuberosities, and lumbar, tibial and thoracic regions. There was no significant change in HR and RT (p>0.05). Fentanyl increased the RR at T20 compared to T0 (0<0.05). At right dorsal quadrant, morphine alone and association reduced ileocecal discharge at T025 and T1 (p<0.05). At left dorsal quadrant, the association differed between the drugs alone at T1, and morphine alone and in combination reduced motility compared to T0 at T025 and T1 (p<0.05). At right ventral quadrant, the association reduced intestinal motility at T025 and T1, and morphine increased it at T12 compared to T0 (p<0.05). At left ventral quadrant, coadministration reduced intestinal motility compared to other drugs, and in relation to T0, morphine reduced in T025, T1 and T4; and coadministration at T025 and T1 (p<0.05). There was mild sedation at T1 when morphine alone and combination were used (p<0.05). Ataxia increased in relation to T0 at T025, T1 and T4 in all treatments and morphine alone at T12 (p<0.05), differing between groups at T025 at association (p<0.05). Antinociception increased mainly on the interval between T1 and T4, which the cold stimulus promoted higher antinociceptive scores in sciatic, coxal and lumbar dermatomes; under hot stimulus all dermatomes detached; on the other hand, mechanical antinociception was better observed in the coxal and lumbar dermatomes (p<0.05). It was concluded that fentanyl showed less physiological effects, morphine had more potent antinociceptive effects in the first 12 hours and the association showed inter.
- ItemSuplementação com doses crescentes de selênio associado à vitamina E no tratamento de bovinos com hematúria enzoótica(Universidade Federal do Espírito Santo, 2023-03-10) Moreira Júnior, Carlos Alberto; Nunes, Louisiane de Carvalho; https://orcid.org/0000000349240614; http://lattes.cnpq.br/8222729734090366; https://orcid.org/0009-0001-1259-7997; http://lattes.cnpq.br/9151124478998136; Almeida, Marco Túlio Costa; https://orcid.org/0000-0001-8730-0425; http://lattes.cnpq.br/7297285577963405; Guerson, Yuri Barbosa; https://orcid.org/0000-0001-7796-0921; http://lattes.cnpq.br/6290312600632479Enzootic bovine hematuria (EBH) is a clinical form of poisoning in cattle caused by the consumption of Pteridium sp. (bracken fern). This disease has a high prevalence in the south of the state of Espírito Santo, Brazil and has no treatment. However, supplementation using selenium (Se) and vitamin E has been shown to be viable. Thus, the aim of this work was to evaluate the effects of supplementation with increasing doses of selenium associated with vitamin E in cattle with enzootic hematuria, as well as to compare selenium levels in whole blood and blood serum and to evaluate the relative activity of the monoamine enzyme oxidase (MAO) in the serum of these animals. For this, 51 whole blood samples and 68 serum samples from 18 cattle with a clinical form of EBH were analyzed.The experimental design was a completely randomized design, with four groups distributed, namely: control group (saline solution), treatment group 1 (0.05 mg/Kg of Se), treatment group 2 (0.10 mg/kg of Se) and treatment group 3 (0.20 mg/Kg of Se), all groups received 500mg/animal of vitamin E in association. Parenteral supplementation was performed intramuscularly for 13 weeks. Biweekly evaluations were performed regarding the intensity of hematuria and weight gain, hematocrit, total plasma proteins (TPP), plasma fibrinogen, Se and relative MAO activity. For statistical analysis were used Kruskal Wallis and Friedman tests with a significance level of 5%, both followed by Dunn's multiple comparison test. It was found that there was no significant difference in weight between the different groups, but there was a reduction in weight over time in the control group. However, there was a significant difference in the intensity of hematuria in the treatment group 1 in relation to the control group after three weeks of supplementation, though, there was no significant difference in the intensity of hematuria over time. There was a significant increase in hematocrit in the treatment group 1 compared to the control group after seven weeks of supplementation, but there was no difference over time in each treatment. For PPT and Fb, no significant differences were observed either between treatments or in relation to time. Se levels were higher in treatment group 1, reaching the highest concentration at serum M8. Relative MAO activity did not differ between groups, but showed a significant reduction over time in each treatment. The supplementation of dairy cattle affected by EBH, using a dose of 0.05 mg/kg selenium associated with vitamin E, improved the clinical sign of hematuria and increased the hematocrit of the animals after three and seven weeks of supplementation, respectively. Serum proved to be the best place to measure Se and there was an increase in the concentration of this element in the eighth week of supplementation. In addition, there was a reduction in MAO activity that can be attributed to vitamin E supplementation, with no influence of different doses of Se.