Doutorado em Genética e Melhoramento

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17475

Nível: Doutorado
Ano de início: 2013
Conceito atual na CAPES: 5
Ato normativo: Homologado pelo CNE/CES Parecer nº 250/2014, portaria nº 187 de 06/03/2015, publicado no DOU de 09/03/2015 seção 1, página 11
Periodicidade de seleção: Semestral
Área(s) de concentração: Genética e Melhoramento
Url do curso: https://geneticaemelhoramento.ufes.br/pt-br/pos-graduacao/PPGGM/detalhes-do-curso?id=1416

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Navegando Doutorado em Genética e Melhoramento por Autor "Carrijo, Tatiana Tavares"

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    Cariótipo e conteúdo de DNA nuclear de Passiflora L.: uma contribuição para sistemática e evolução do gênero
    (Universidade Federal do Espírito Santo, 2018-08-27) Ferreira, Darley Aparecido Tavares; Fontes, Milene Miranda Praça; Karsburg, Isane Vera; Miranda, Fábio Demolinari de; Carrijo, Tatiana Tavares; Oliveira, Stefani Cristina de
    Passiflora L. comprises five subgenera subdivided into subdivided into 16 supersections, 31 sections and 13 series. The genus has been studied in several aspects, botanical, systematic and evolutionary. However, its delimitation at the infrageneric level is still subject to discussion. Classical karyotype data and the size of the nuclear genome have been considered informative for the refinement of this knowledge. Based on this premise, the present study had two focus: a) revisiting, expanding and updating the information on karyotype and nuclear value 2C in species belonging to the subgenus Astrophea, Decaloba, Deidamioides and Passiflora. b) Analyze the data in the viewpoint of the systematic and evolution. As result, chromosome number knowledge was expanded for nine species and confirmed for 19. Chromosomes class was determinated for 19 species and reevaluated for nine. In all species analyzed the karyogram was assembled, being in 24 taxa for the first time and in four updated. Chromosome number counts revealed 2n = 12 for the subgenus Decaloba, 2n = 18 and 2n = 20 for the subgenus Passiflora and 2n = 24 on the subgenera Astrophea and Deidamioides and the unpublished 2n = 48 chromosomes in P. contracta (subgenus Deidamioides). Chromosome class ranged between metacentrics and submetacentrics pairs with exception of some acrocentrics in P. lindeniana (two pairs) and P. arborea (three pairs), both of the subgenus Astrophea. This presence of acrocentrics chromosomes evidenced probable occurrence of dysploidy within the genus. The knowledge of genome size in the genus Passiflora was also extended to 19 species and updated to 22. Nuclear 2C value exhibited differences up to 925% betweem species, where the lowest value found was 0.59 pg (P. capsularis - Decaloba) and the greater 5.46 pg for (P. quadrangularis - Passiflora). For some species the increase in genome size is correlated with the increase of the chromosome number, a consequence of the polyploidy. Already in others, this relation was not observed suggesting the occurrence of structural rearrangements. In conclusion, the karyotype and genome size of the genus Passiflora suggest diversification by polyploidy and disploidia. The number x = 6 is probably the ancestral chromosome number of the genus. Moreover, the data analyzes were complementary with systematic approaches of the genus and provided support for the current subgeneric classification of this taxon.
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    ECO-FRIENDLY HERBICIDE POTENCIAL OF DIFFERENT Psidium SPECIES: MECHANISMS OF ACTION, PHYTOTOXICITY AND CYTOGENO-TOXIC APPROACHES
    (Universidade Federal do Espírito Santo, 2021-02-26) Alves, Thammyres de Assis; Fontes, Milene Miranda Praca; https://orcid.org/0000000177389518; http://lattes.cnpq.br/1372644511398549; https://orcid.org/0000000311484832; http://lattes.cnpq.br/; Oliveira, Stefanie Cristina de; https://orcid.org/; http://lattes.cnpq.br/; Carrijo, Tatiana Tavares; https://orcid.org/0000000283660738; http://lattes.cnpq.br/0479854777557016; Vieira, Larissa Fonseca Andrade; https://orcid.org/; http://lattes.cnpq.br/
    Since the beginning of agronomic activities, diseases, pests, and weeds started to appear. Thus, development of agrochemicals was especially important for increasing agricultural productivity, resulting in lower costs and increased efficiency of productio
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    Psidium cattleyanum Sabine: a influência do conteúdo de DNA na formação de quimiotipos e na atividade em diferentes modelos biológicos
    (Universidade Federal do Espírito Santo, 2019-12-19) Spadeto, Micheli Sossai; Fontes, Milene Miranda Praca; https://orcid.org/0000000177389518; http://lattes.cnpq.br/1372644511398549; https://orcid.org/0000-0002-8198-1378; http://lattes.cnpq.br/6497624091795405; Menini, Luciano; https://orcid.org/0000000336565428; http://lattes.cnpq.br/2646344913961349; Oliveira, Stefanie Cristina de; https://orcid.org/0000-0001-8350-2601; http://lattes.cnpq.br/6404885772178652; Carrijo, Tatiana Tavares; https://orcid.org/0000000283660738; http://lattes.cnpq.br/0479854777557016
    Polyploidy is defined as the presence of two or more chromosomal assemblies in an organism and has been reported as one of the most important mechanisms of plant speciation and diversification, and may result from the fusion of two genomes of distinct species (alopoliploidia) or genomes of individuals of the same species (autopolyploidy). Gene duplication in polyploid plants generates high inter and intraspecific variability, which may influence the production of secondary metabolites and, consequently, the chemical composition of essential oils and the formation of different chemotypes. Within the genus Psidium (basic chromosome number x = 11), some species have been described as polyploids, among them, Psidium cattleyanum Sabine. For this species, cytogenetic studies reported nine cytotypes, with chromosome numbers ranging from 2n = 22 to 2n = 132 chromosomes. In this context, considering the existence of this variation in ploidy in P. cattleyanum, the present work aimed to study the influence of this variation on the qualitative and quantitative production of different essential oil chemotypes and also to relate the different DNA contents with the biological activity of these oils in Lactuca sativa, Sorghum bicolor and Aedes aegypti. Through flow cytometry it was possible to observe differences in the DNA content of the analyzed plants and by chromatographic profile analysis were also identified three distinct chemotypes. Plants with higher DNA content (6.03 and 5.81pg) showed higher yield and are mostly composed of hydrogenated sesquiterpenes, while plants with lower DNA content (3.95, 3.23, 3.80 and 3.20pg) produce less oil and are composed. by hydrogenated monoterpenes. In addition, a plant with a median DNA content (4.71pg) compared to the others presented intermediate yield and composition between the two previous groups. Thus a direct relationship between the DNA content and the qualitative and quantitative characteristics of the essential oils of Psidium cattleyanum was observed. In the vegetable and larvicide bioassays, the oils showed activity and demonstrated potential as bioherbicides and biolarvicides.
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    The epigenetic and physiologic changes as a trigger for indirect somatic embryogenesis in Coffea canephora
    (Universidade Federal do Espírito Santo, 2019-07-15) Silva, Paulo Marcos Amaral; Clarindo, Wellington Ronildo; https://orcid.org/0000000288268620; http://lattes.cnpq.br/0339970047422716; https://orcid.org/; http://lattes.cnpq.br/2853274010679824; Fontes, Milene Miranda Praca; https://orcid.org/0000000177389518; http://lattes.cnpq.br/1372644511398549; Mendonca, Maria Andreia Correa; https://orcid.org/0000-0002-3798-3309; http://lattes.cnpq.br/3412339192891083; Sanglard, Natalia Arruda; https://orcid.org/0000-0003-3219-8616; http://lattes.cnpq.br/7809043431671687; Carrijo, Tatiana Tavares; https://orcid.org/0000000283660738; http://lattes.cnpq.br/0479854777557016
    Indirect somatic embryogenesis (ISE) establishment for Coffea species started in the 1970s. Since then, intraspecific variations in the morphogenic pathway have been reported, despite the meticulous standardization of the environmental conditions in vitro. Several authors have suggested that these variations are the result of genetic, epigenetic and/or physiological events, highlighting the need for investigations to know the respective causes. Along these lines, this study aimed to investigate and describe, for the first time, the changes in and influence of epigenetic and physiological aspects during cell proliferation and somatic embryo regeneration from cell aggregate suspensions of Coffea canephora. The cell proliferation step was characterized by increase in cell mass in all subcultures; relatively low mean values of global 5-methylcytosine (5-mC%), abscisic acid (ABA) and indole-3-acetic acid (IAA); high mean value of 1-aminocyclopropane 1-carboxylic acid (ACC, an ethylene precursor); and increases followed by decreases in spermidine (Spd, a polyamine) levels. With regard to the in vitro conditions, these aspects promoted the cell proliferation, showing that the epigenome and metabolome are fundamental for the first morphogenic moment of ISE. In turn, the second ISE moment is correlated with epigenetic and physiological changes. The competence acquisition, determination and cell differentiation steps were marked by increases in mean values of 5-mC%, IAA and ABA and decreases in ACC and Spd, evincing that these epigenetic and physiological changes are the triggers for regeneration and maturation of somatic embryos. Therefore, dynamic and coordinated epigenetic and physiological changes directly affect the two morphogenic moments of C. canephora ISE.
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    Toxicidade de compostos químicos derivados de fenol natural em bioensaios vegetais
    (Universidade Federal do Espírito Santo, 2023-09-18) Costa, Thaís Lazarino Maciel da; Soares, Tais Cristina Bastos; https://orcid.org/0000000163567993; http://lattes.cnpq.br/6580031598802359; https://orcid.org/0000-0002-8671-958X; http://lattes.cnpq.br/7735534676626922; Souza, Tatiana da Silva; http://lattes.cnpq.br/0969903694890602; Carrijo, Tatiana Tavares; https://orcid.org/0000000283660738; http://lattes.cnpq.br/0479854777557016; Sobreira, Franciele Barros de Souza; Karsburg, Isane Vera
    Excessive use of herbicides causes problems such as impacts on human health, environmental pollution and plant resistance to conventional herbicides. Research is seeking safer and more effective alternatives, such as natural compounds, semi-synthetic derivatives and essential oils. This study focused on the evaluation of the toxicity and genotoxicity of synthetic molecules derived from thymol and rosemary essential oil, both rich in thymol. All compounds were subjected to phytotoxic and cytotoxic activity tests using Lactuca sativa and Sorghum bicolor as model plants. In addition, the genotoxicity of rosemary oil was evaluated through molecular analyses with ISSR markers. The germination percentage, germination speed index, aerial and root length of seeds and seedlings subjected to treatments were evaluated. Molecules containing chlorine in the para position of the thymoxy group exhibited phytotoxic and cytogenotoxic effects superior to those of the commercial herbicides 2,4-D and glyphosate. Concentrations of 0.01 and 0.0050 mmol. L-1 of rosemary essential oil promoted greater toxicity responses for both lettuce and sorghum, presenting a clastogenic and aneugenic mechanism of action. Molecular analyses demonstrated equality between the genomic stability of plants treated with the tested positive controls and the different concentrations of the oil. The results demonstrate the phytotoxic, cytotoxic and genotoxic effects of the synthetic molecules and the rosemary essential oil.