Mestrado em Bioquímica

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http://repositorio.ufes.br/handle/10/17562

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Navegando Mestrado em Bioquímica por Autor "Bem, Daniela Amorim Melgaço Guimarães do"

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    Avaliação da expressão dos genes DNAHC6, DYNLT1, Pcdh21, SIGMAR1, Lingo1 e Gabra2 como marcadores moleculares na Doença de Huntington
    (Universidade Federal do Espírito Santo, 2018-09-27) Mendonça, Suellen Rosseto; Pires, Rita Gomes Wanderley; Bem, Daniela Amorim Melgaço Guimarães do; Silva, Cristina Martins e; Muniz, Marcelo Ramos; Errera, Flavia Imbroisi Valle
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    Caracterizações bioquímica e hemostática de pacientes com diabetes Mellitus Tipo 2 em insulinização
    (Universidade Federal do Espírito Santo, 2014-07-15) Gonçalves, Nadmy Arrivabene Zavaris; Bem, Daniela Amorim Melgaço Guimarães do; Vassalo, Breno Valentim; Gomes, Isabele Beserra Santos
    The study aims to characterize biochemically and hemostatic mellitos patients with diabetes mellitus type 2 (DM2) who developed insulin regimen. Composed group under analysis, 40 patients attended the Basic Health Unit of Consolation, Vitória / ES, aged 25 to 80 years, diagnosed with DM2 and that were already evoluted to insulinization. As controls, 40 patients were selected in the same age group without laboratory and / or clinical diagnosis of DM. Markers of inflammation, hypercoagulability and fibrinolysis were measured: Fibrinogen, D-dimer (D-Di) and plasminogen activator type 1 inhibitor (PAI-1). The polymorphism (-675 4G/5G) in the promoter region of the PAI-1 gene was correlated with their serum levels. Biochemical parameters were measured: plasma glucose (PG), glycated hemoglobin (A1C), total cholesterol (TC), HDL cholesterol (HDL-C), LDL (LDLc) cholesterol, triglycerides (TGC), ultrasensitive C reactive protein (hsCRP), urea and serum creatinine. There was still checking BMI, obesity, smoking, hypothyroidism, hypertension, dyslipidemia and insulin resistance. Statistical analysis showed significant differences (p <0.05) between groups with regard to mean values of HDLC, VLDL-C, TGC, urea, hsCRP and fibrinogen. There was significant difference between groups for VLDLc, TGC, creatinine and fibrinogen. Controls present correlations between: fibrinogen and glucose, hsCRP and fibrinogen, PAI-1 and glucose, PAI-1 and BMI. In insulinization DM2 group correlation was observed correlation between: fibrinogen and D-di, hsCRP and fibrinogen, D-Di and glucose (negative), PAI-1 and triglycerides, PAI-1 and BMI. PAI-1 levels were higher in the control group in subjects with genotype 5G5G, 4G5G and 4G4G followed. Binary Logistic Regression confirmed that the variables hypertension and fibrinogen were significant at p-value (0.009) and (0.049) and adjusted odds ratio (4.184, 1.426 to 12.276) and (3.293, 1.006 to 10.775), respectively, showing that hypertensive patients have a risk 4.18 times more likely to have insulinization type 2 diabetes and that individuals with hyperfibrinogenemia have a 3.29 times greater risk. With this study we hope to contribute to better understanding of the complex changes that accompany the user insulinization type 2 diabetic patients in expectation of seeking appropriate treatment and prevention for the macrovascular complications of diabetes.
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    Efeitos de inibidores da DNA metiltransferase na modulação de comportamentos aversivos relacionados à ansiedade
    (Universidade Federal do Espírito Santo, 2017-12-15) Oliveira, Mayana Cardoso de; Harres, Vanessa Beijamini; Bortoli, Valquíria Camin de; Rodrigues, Lívia Carla de Melo; Bem, Daniela Amorim Melgaço Guimarães do
    Anxiety disorders such as generalized anxiety disorder (GAD) and panic disorder (PT), cause significant impacts on the lives of patients affected by them. Pharmacological treatment of GAD and PT presents problems of efficacy and safety. In this sense, new pharmacological targets have been studied in order to develop more effective treatment options. The pathogenesis of anxiety disorders is complex in nature and interacts with environmental and biological factors, particularly genetic factors. Recently, it has been shown that epigenetic processes can influence gene regulation and mediate adaptation to environmental factors in mental disorders, characterized by a stable hereditary phenotype resulting from changes in the chromosome without changes in the DNA sequence. Epigenetic modifications include changes in DNA, such as methylation, which consists of the addition of a methyl (CH3) catalyzed enzyme by DNA methyltransferases (DNMTs).