Mestrado em Bioquímica
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- ItemAvaliação Físico-Química Da Interação De Betaciclodextrina Com Isoformas Alfa- E Psi- Tripsina(Universidade Federal do Espírito Santo, 2024-02-26) Oliveira, Daniel de Jesus de; Rosa, Dayanne Pinho; https://orcid.org/0000-0002-1483-5848; http://lattes.cnpq.br/7576001371663181; Santos, Alexandre Martins Costa; https://orcid.org/0000-0002-8801-8875; http://lattes.cnpq.br/4144105396879016; https://orcid.org/0009-0007-0082-9547; http://lattes.cnpq.br/5786173815850998; Araujo, Marlonni Maurastoni ; https://orcid.org/0000-0002-6064-3126; http://lattes.cnpq.br/6052184072658200; Cicilini, Maria Aparecida ; https://orcid.org/0000-0003-2751-117X; http://lattes.cnpq.br/7082425279468970Cyclodextrins are cyclic polysaccharides with a structure composed of glycopyranose. Studies have shown that cyclodextrins can form promising interactions with proteins, which has several applications in biotechnology and biochemistry, such as the solubilization of hydrophobic compounds and the protection of compounds sensitive to thermal, oxidative degradation, among others. In this work, in order to evaluate the influence of this polysaccharide on conformational stability and protein activity, physicochemical tools were used, adopting bovine trypsin as an enzymatic model of study, due to the knowledge of its structure and enzymatic activity. This enzyme belongs to the class of serine proteases and has several isoforms, α-trypsin, β-trypsin and ψ-trypsin being the most studied. The results showed that protease amidasic activity increased up to about 76% in commercial trypsin, 82% in alpha isoform, and 45% in psi isoform, suggesting that the addition of β-cyclodextrin optimized the amidsis activity of the trypsin isoforms tested. Fluorescence and absorption spectroscopy revealed changes in the structural properties of trypsin due to the interaction with βcyclodextrin, affecting the activity and conformational stability of trypsin. The formation of supramolecular states of trypsin isoforms in the presence of β-cyclodextrin by means of dynamic light scattering (DLS) was also evaluated, in which an increase in the size and dispersivity of the molecule suggestive of the formation of supramolecular states was verified. The joint evaluation of these results suggested that there was the formation of stabilizing interactions between β-cyclodextrin and trypsin isoforms through the formation of supramolecular states, with possible formation of inclusion complexes. Thus, it is suggested that β-cyclodextrin may interact with trypsin and affect its proteolytic activity and stability and thus the influence of the osmolyte can be understood at the molecular level.
- ItemO EFEITO DA FNDC5 E IRISINA EM LINHAGENS DE CÉLULAS DE CÂNCER DE MAMA MCF-7 E MDA-MB-231(Universidade Federal do Espírito Santo, 2023-09-01) Junior, Pedro Florencio da Cunha Fortes; Daltoe, Renata Dalmaschio; https://orcid.org/0000000285710428; http://lattes.cnpq.br/0931389996547944; https://orcid.org/; http://lattes.cnpq.br/; Sousa, Nuno Manuel Frade de; https://orcid.org/; http://lattes.cnpq.br/; Madeira, Klesia Pirola; https://orcid.org/; http://lattes.cnpq.br/; Goncalves, Juliana Barbosa Coitinho; https://orcid.org/000000025892050X; http://lattes.cnpq.br/3448669742301744abstract
- ItemDesenvolvimento de um biossensor para detecção de Salmonella em alimentos(Universidade Federal do Espírito Santo, 2023-07-06) Silva, Gabrielle Batista Landim; Oliveira, Jairo Pinto de; https://orcid.org/0000000175951183; http://lattes.cnpq.br/2228283301316218; https://orcid.org/0009-0004-1921-6060; http://lattes.cnpq.br/2195941577218775; Xavier, Andre da Silva; https://orcid.org/0000000292510301; http://lattes.cnpq.br/5661020509713522; Nascimento, Isabella Sampaio do; https://orcid.org/0000-0002-4711-123X; http://lattes.cnpq.br/8257371551653352Salmonellosis is a disease transmitted by contaminated food and is one of the main causes of infections worldwide. To ensure public health, early detection of pathogens is crucial. However, current detection methods are laborious and time-consuming, impacting the entire food chain. In this study, we developed a biosensor for rapid detection of Salmonella in food samples. The detection system is based on lateral flow technology with gold nanoparticles as an optical transducer and antibodies as antigen recognition molecules, with results obtained in just 15 minutes after inserting the strip into the sample. The developed biosensor has selectivity in relation to other microorganisms such as Escherichia coli and Staphylococcus aureus, sensitivity, with a visible detection limit of 10³ CFU.ml⁻¹ and quantitative of 1 CFU.ml⁻¹, values below existing commercial tests and 100% of repeatability. It is fast and reliable and can be a favorable alternative for Salmonella screening in food to ensure food safety.
- ItemDESENVOLVIMENTO DE UM IMUNOENSAIO DE FLUXO LATERAL PARA A DETECÇÃO DE PARAQUAT EM AMOSTRAS DE ALIMENTOS(Universidade Federal do Espírito Santo, 2022-12-16) Jara, Marcia Daniela Lazo; Oliveira, Jairo Pinto de; https://orcid.org/0000000175951183; http://lattes.cnpq.br/2228283301316218; https://orcid.org/; http://lattes.cnpq.br/; Guimaraes, Marco Cesar Cunegundes; https://orcid.org/0000000321460180; http://lattes.cnpq.br/0261991057482057; Flórez, Francisco Javier Burgos; https://orcid.org/; http://lattes.cnpq.br/abstract
- ItemA EXPOSIÇÃO CRÔNICA AO MERCÚRIO AGRAVA OS EFEITOS DO INFARTO AGUDO DO MIOCÁRDIO EM RATOS(Universidade Federal do Espírito Santo, 2022-07-01) Souza, Keren Alves de; Soares, Aurelia Araujo Fernandes; https://orcid.org/0000000199451909; http://lattes.cnpq.br/5478728158150003; https://orcid.org/; http://lattes.cnpq.br/; Campos, Fabiana Vasconcelos; https://orcid.org/; http://lattes.cnpq.br/; Langa, Paulina; https://orcid.org/; http://lattes.cnpq.br/abstract