Mestrado Profissional em Clínica Odontológica

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Análise Comparativa entre Leucoplasia Oral e Leucoplasia Verrucosa Proliferativa (LVP) e Caracterização da Imunoexpressão da Citoqueratina-19 na LVP
(Universidade Federal do Espírito Santo, 2020-05-15) Fonseca, Emanuely Ronconi da; Barroso, Danielle Resende Camisasca; https://orcid.org/; http://lattes.cnpq.br/3989074593181674; https://orcid.org/; http://lattes.cnpq.br/; Barros, Liliana Aparecida Pimenta de; https://orcid.org/; http://lattes.cnpq.br/8635277660080840; Gouvea, Sonia Alves; https://orcid.org/000000015180471X; http://lattes.cnpq.br/7268228122543743
Objectives: To survey and review the cases diagnosed as oral leukoplakia (OL) at the UFES Department of Oral Pathological Anatomy, from 2009 to 2017, describe the clinical and socio-demographic histopathological characteristics, apply the criteria for pro
Análise da expressão de Patched e distribuição de mastócitos em tumores odontogênicos ceratocísticos
(Universidade Federal do Espírito Santo, 2014-12-18) Milholli, Ana Flávia Lagassa; Barros, Liliana Aparecida Pimenta de; Coburn, Karla Loureiro Almeida; Spohr, Tânia Cristina Leite de Sampaio e
The Keratocystic Odontogenic Tumor (KCOT) is an intraosseous benign tumor, which displays aggressive behavior, with a tendency to local destruction and infiltrative and recurrent potential. It is also one of the manifestations of the Nevoid Basal Cell Carcinoma Syndrome (NBCCS). Evidence shows that the patched gene may be a significant factor in the development of KCOTs by having their activity inactivated. The aim of this study was to perform a comparative analysis between sporadic and syndromic KCOTs by checking the expression pattern of Patched protein and the distribution pattern of mast cells. Twenty-eight specimens of KCOTs recorded in Anatomic Pathology Service (SAP) of the School of Dentistry of the Federal University of Espirito Santo (UFES) during the period 2004-2012 were submitted to immunohistochemical technique to detect Patched protein and stained with Alcian Blue to detect mast cells. Histopathologic features were evaluated. All clinical data (gender, age, ethnicity and location of the lesion) were obtained from the files. For immunohistochemical analysis a score was adopted. Regarding the quantitative expression of patched, we found that 77.77% of KCOTs (syndromic and non-syndromic) showed immunostaining score of 4 (between 76% and 100% of cells stained). The basal and intermediate epithelial layers comprised most of staining compared to the surperficial layer. Only cytoplasmic staining was found. The histopathologic features were more evident in syndromic tumors, however, there was no significant difference in mean mast cells found to syndromic and non-syndromic tumors. Therefore, our findings demonstrated a strong epithelial expression of Patched in KCOTs, suggesting that this protein seems to be important for the pathogenesis of these tumors.
Análise epidemiológica, histopatológica e imuno-histoquímica de ameloblastomas : casuística de seis anos
(Universidade Federal do Espírito Santo, 2012-07-04) Rocha, Regina Furbino Villefort; Barros, Liliana Aparecida Pimenta de; Coburn, Karla Loureiro Almeida; Freitas, Vanessa Morais
Ameloblastomas are odontogenic tumors (OTs) derived from epithelium which etiology remains unknown. However, recent studies have identified molecular changes associated with the development and progression of OTs, including cell adhesion molecules like E-cadherin and beta-catenin. Objectives: to conduct an epidemiological investigation of ameloblastomas cases from files of the Anatomical Pathology Service at Federal University of Espírito Santo (SAPB-UFES), analyze their histopathological features and the expression of beta-catenin in different variants of ameloblastomas. Methods: a retrospective study of ameloblastomas registered at SAPB-UFES between March 2004 and December 2010. Sociodemographic, clinical and imaginological data were collected, as well as data about access, diagnosis, treatment and follow up of these patients. The histopathological analyzes were based on Vickers and Gorlin, Waldron and El-Mofty and the World Health Organization criteria. Primary antibody anti beta-catenin mouse monoclonal and indirect immuno-peroxidase technique was employed for immunohistochemical analysis. Intensity and location of the immunostaining were analysed. For semiquantitative analysis the scores were: negative, focal, variable and uniformity positivity. Results: there were 13 ameloblastomas, histopathologically classified as solid (06), unicystic (03) and desmoplastic (03). All of them were immunostained. The intensity of immunostaining ranged from weak to strong (1-3). The mean of immunostaining ranged from 10.82% to 13.38% in the nucleus; from 39.93% to 47.61% in the membrane; and from 90.01% to 98.53% in the cytoplasm. However, there was no significant difference in expression of beta-catenin between three different types of ameloblastomas. Conclusion: The results were similar to other epidemiological studies. The cytoplasmic expression of beta-catenin shows accumulation in the cytoplasm and suggests changes in the Wnt signaling pathway. Moreover, the reduction of membrane expression suggests changes in cell adhesion.
Análise imuno-histoquímica do colágeno tipo I em carcinoma de células escamosas orais e suas lesões precursoras
(Universidade Federal do Espírito Santo, 2017-03-09) Fanchiotti, Renata Escapini; Barros, Liliana Aparecida Pimenta de; Souza, Letícia Nogueira da Gama de; Barroso, Danielle Resende Camisasca; Pacheco, Marcos da Silva
Squamous cell carcinoma (SCC) is the most common cancer of the mouth, and is still associated with high mortality rates. Besides the evaluation of the clinical aspects, it is fundamental to perform a more accurate analysis of the molecular profile to better understand its biological behavior. Type I collagen is one of the main components of the extracellular matrix (ECM) and has been associated with the process of tumorigenesis by participating in events such as angiogenesis and metastasis. The present study performed an immunohistochemical analysis of the type I collagen profile in lesions diagnosed by the Department of Oral Pathology of the Dental School / UFES. The groups evaluated were: 10 cases of low risk lesions; 10 cases of high risk of malignancy and 30 cases of SCC. Also, associations were established between the markers and the clinical profile of the patient and the tumor. Our findings showed that men were the most affected in the lesions at high risk (60%) and SCC (86.6%), while women represented the majority in low risk lesions (80%). In relation to the affected sites, the jugal mucosa was the most frequent in the low risk lesions (50%), and the tongue in lesions of high risk (60%) and SCC (43.3%). As for the profile of type I collagen, the fibers were moderately irregular (C ++) and strongly marked. In the low risk lesions, the three types of fiber disposition were observed, and with weak marking; And those at high risk, the fibers were fine, regular and markedly strong. When the association between clinical and microscopic findings was found, it was possible to observe that patients smokers with SCC, the fiber pattern was irregular (P = 0.04) and strong marking (P = 0.02) compared to smokers, but diagnosed with lesions Low or high risk. Our findings suggest associations between clinical and microscopic aspects regarding the pattern of deposition of type I collagen during the alterations that occur in the oral mucosa in the process of malignant transformation.
Análise radiográfica das alterações periapicais e periodontais em pacientes com síndrome de sjögren primária
(Universidade Federal do Espírito Santo, 2018-05-30) Braga, Paula Silva; Barros, Liliana Aparecida Pimenta de; Rosetti, Elizabeth Pimentel; Pinel, Roberta Grasselli Batitucci; Vaz, Sergio Lins de Azevedo
The higher incidence of caries and periodontal impairment was described in patients with Primary Sjögren's Syndrome (SSp). As a consequence, radiolucent periapical lesions (RPL) and alveolar bone loss may occur. Despite this, LPR and radiographic alveolar bone loss were little explored in the scientific literature. This study intends to identify radiographically periapical and periodontal changes in patients with SSP. Periapical radiographs were taken for technique of parallel-cone in patients with SSp. The digital radiographs were evaluated by a radiologist through Imagine J Software. A socioeconomic questionnaire was applied. The salivary flow was evaluated through the collection of unstimulated saliva. Periapical region of all teeth, excluding third molars, was examined. In addition, alveolar bone loss was measured radiographically. Participated in the study twenty-four female patients, aged between 20 and 71 years, with xerostomia and 80% of them with hyposalivation. 70.8% of patients had at least one RPL. The mean number of teeth per patient was 23.3 ± 3.1 with prevalence of RPL in teeth of 7.86%. When comparing teeth with and without RPL, the teeth with RPL had a higher percentage root-filled teeth and tooth decay and were located more in the central superior sextant (p <0.005). For bone loss, the prevalence was 33.3%, and the pattern of horizontal bone loss prevailed (94.6%) (p <0.01). The mean age was higher for patients who presented bone loss (59.63 ± 9.14) compared to patients who did not present bone loss (49.60 ± 8.33) (p = 0.015). The horizontal bone loss was more frequent and occurred the mainly anterior region and the right inferior sextant (p <0.05). The most affected teeth were the lower lateral incisor and lower canine, followed by the lateral incisor and lower central incisor (p<0,01).When comparing with other epidemiological studies addressing healthy patients, the prevalence of RPL and alveolar bone loss was higher for patients with SSp.
Avaliação clínica da condição periodontal e de saúde bucal de pacientes com síndrome de sjögren primária
(Universidade Federal do Espírito Santo, 2018-09-19) Zamboni, Jisely Brito; Barros, Liliana Aparecida Pimenta de; Cristo, Valéria Valim; Rosetti, Elizabeth Pimentel
Primary Sjögren's Syndrome (SSp) is a chronic, autoimmune inflammatory disease that causes reduced secretion of the salivary and lacrimal glands. Despite the important role of saliva on oral health, it is still not confirmed whether SSp exerts influence on the periodontal condition of individuals with SSp. The aim of the present study was to evaluate clinically the oral health status of SSp patients compared to a control group. The sample consisted of 29 female patients with SSp (G1) and 30 control patients (G2), matched by age, gender and socioeconomic status. The groups were compared for oral manifestations, unstimulated whole saliva, index of decayed and missing teeth (DMFT), plaque index (PI), gingival index (GI), bleeding probing (BOP), depth of probing (PS), clinical insertion level (CIL) and case definition of periodontitis. Chi square test or Fisher exact test was used for the categorical variables, and for the continuous variables, Student t test for comparison of means. The confidence interval established for all calculations was 95% (p≤0.05). The G1 presented more cases of xerostomia (p = 0.0001), oral candidiasis (p = 0.042) and fissured tongue (p = 0.026). In addition, G1 had a lower average of unstimulated whole saliva (p = 0.001) tooth decay (p = 0.019). The G2 had more cases of flavonoid (p = 0.009), higher mean PI (p = 0.047) and SS (p = 0.026). Although not statistically significant, G1 presented more cases of moderate / severe periodontitis and stage IV periodontitis. In addition, it was the only one to present cases of tooth mobility (13.8%) and suppuration (3.4%). The results obtained did not reach statistical significance to confirm the relationship between SSp and periodontitis. G1 presented lower unstimulated whole saliva, greater caries involvement and oral candidiasis, compared to G2. The groups evaluated were statistically similar in relation to periodontal indices, but a larger number of patients with periodontitis severity were observed in the G1 group.
Avaliação das condições dentárias e periodontais de alcoolistas e não alcoolistas
(Universidade Federal do Espírito Santo, 2018-07-16) Sossai, Lorena Lirio; Feitosa, Alfredo Carlos Rodrigues; Guerra, Selva Maria Gonçalves; Barros, Liliana Aparecida Pimenta de; Gomes, Maria da Penha Zago
Alcohol abuse or dependence is a major risk factor for many illnesses and disabilities. Its chronic use is irritating to the mucosa and is related to worse oral conditions. However, few studies evaluate the oral health of alcohol dependents. The objective of this study is to evaluate the dental and periodontal status of alcoholics and to compare them with the dental and periodontal status of non-alcoholics. This cross-sectional observational study included 60 males, aged between 32 and 72 years, with 30 alcoholics assisted in the Alcoholic Attendance Program of the Hospital Universitário Cassiano de Moraes and 30 non-alcoholics attended at the Periodontia Nucleus of the Federal University of Espírito Santo Holy. These were submitted to the interview and clinicai examinations. Statistical analysis was characterized by means of frequency, percentage, mean, standard deviation, multiple logistic regression and Chi-square and Studenfs t-tests. The alpha levei of significance used in all analyzes was 5% with a 95% confidence interval. Alcoholics had a lower average age, used more tobacco, had a lower frequency of dental brushing and flossing, had more lost and decayed teeth, more residual root and more teeth with visible plaque index present (OR = 4.9 - 4.4 to 5.5) compared to nonalcoholics. The site with a clinicai insertion levei of 4 mm or more had half the chance of being detected (OR = 0.50 - 0.43 to 0.57) in alcoholics compared to nonalcoholics. There was no difference in the presence of the periodontal pocket of the alcoholic patients in relation to the abstinence time; however, there was a difference between the use of tobacco (p <0.001) and the time of alcohol use (p <0.001). Alcoholic individuais presented higher prevalence of caries, fewer sites with periodontal disease and worse oral hygiene than non-alcoholic individuais. Regarding professional follow-up, there was similarity between the groups evaluated.
Caracterização da expressão molecular da podoplanina e do Ki-67 nas displasias epiteliais e carcinomas de células escamosas orais : análise da transformação maligna
(Universidade Federal do Espírito Santo, 2015-12-18) Ribeiro, Fabiano de Azevedo; Souza, Leticia Nogueira da Gama de; Barros, Liliana Aparecida Pimenta de; Barroso, Danielle Resende Camisasca; Lourenço, Simone de Queiroz Chaves
Introduction: Oral squamous cell carcinoma (OSCC) is the most common malignancy in the oral cavity and its development involves a series of molecular mechanisms and may be preceded by oral potentially malignant disorders (OPMDs). Biomarkers expressed on the cell proliferation phase and involved in the invasiveness may lead to a better understanding of the carcinogenesis process. Objectives: To characterize the Ki-67 expression and D2-40 in OPDM and OSCC and their clinically healthy margins and confront the histopathological degrees and clinicopathological data from these lesions. Methods: In this cross-sectional study, we evaluated 25 OPDM, 10 OSCC and their perilesional tissues from May-2013 to July-2014 in the Oral Diagnosis Center. The morphometric alterations were evaluated in OPDM and OSCC by hematoxylin-eosin method and the expression of Ki-67 and podoplanin, qualitatively and quantitatively, by immunohistochemistry. Results: For the group of OPDM, most lesions showed no dysplastic changes on its margins, already in OSCC, 50% had severe OED and 20% moderate and 30% mild OED. In immunohistochemical analysis, compared the lesions and their perilesional tissues, Ki-67 showed a difference in OPDM (p = 0.016) between lesions OPDM and OSCC(p=0.006) and between perilesional tissue and peritumoral tissue (p=0.001). There was a direct relationship between higher degree of OED and greater expression of Ki-67 in OPDM (p=0.010) and its peritumoral tissues (p=0.022). The podoplanin showed higher expression rate in the lesions themselves than in their perilesional and peritumoral tissues, although not significantly different. There was a direct relationship (p=0.001) between severity of OED and expression of podoplanin in OPDM lesions and positive correlation between expression of Ki-67 and podoplanin in OSCC lesions(p=0.000). Conclusions: It was found a gradual increase in ascending order of the expression of molecular markers studied in perilesional tissue, OPDM, peritumoral tissues and OSCC. Besides the significant correlation between cell proliferation and the degree of oral epithelial dysplasia, and the degree of differentiation of OSCC, suggesting Ki-67 as an additional criterion for determine the severity of OPDM. The positive correlation between Ki-67 and D2-40 in OSCC points them as prognostic biomarkers and therapies targeted against cancer. Further studies may reveal a better participation of podoplanin in carcinogenesis.
Efeito do recobrimento de bráquetes ortodônticos com filme de fosfato de cálcio na dureza do esmalte bovino submetido à desmineralização in vitro
(Universidade Federal do Espírito Santo, 2019-03-26) Fiorotti, Luiz Felicio Fernandes Louzada; Zanon, Juliana Malacarne; https://orcid.org/0000-0001-7913-4973; http://lattes.cnpq.br/4671962861662301; https://orcid.org/0000-0003-4285-9983; http://lattes.cnpq.br/9667978795842551; Barros, Liliana Aparecida Pimenta de; https://orcid.org/; http://lattes.cnpq.br/8635277660080840
Despite the widespread use of fixed orthodontic appliances to correct malocclusions, White spot lesion (WSL) is routinely adverse effects associated with this treatment modality. Thus, the aim of this in vitro study was to evaluate the calcium leaching ability of calcium phosphate (CaP) thin film coated orthodontic brackets in distilled water, as well to evaluate the surface Vickers nanohardness (VHN) of experimental bracket coated with calcium phosphate (CaP) after demineralizing challenge. For this, original manufacturer's orthodontic brackets were used as control (n=15) and fifteen were coated with CaP film by electrodeposition. The film was characterized morphologically by scanning electron microscopy (SEM) and dispersive energy spectroscopy (EDS) for elemental analysis. Twenty-eight coated brackets were stored in distilled water for 7, 15 and 30 days and analyzed at 7, 15 and 30 d for calcium leaching. Thirty blocks (8 x 10 mm) of enamel, obtained from the vestibular face of healthy bovine incisors and submitted to metallographic polishing, were used. The VHN of bovine enamels was evaluated before and after demineralizing challenge using 50 mN of force, for 15 seconds, in five previously determined peripheral areas. Then, the enamel blocks were divided into 2 groups (n = 15) according to the protocol that was attached to the dental enamel: test group, CaP coated bracket; and control group, manufacturer's original brackets. For the union of the rinses to the bovine bone, the system Transbond XT was used in all the collages. After storage in distilled water at 37 °C for 24 h, the specimens underwent a demineralization process in pH 5.0 solution for 32 hours at 37 °C. Soon after the VHN was remeasured. In the SEM the surface of the coating was amorphous and crystalline, in both structures there is presence of calcium and phosphate, proving the coating. Calcium leaching occurred with greater intensity in 7 d (p
Estudo comparativo das anomalias dentárias em crianças portadoras e não portadoras da síndrome de Down
(Universidade Federal do Espírito Santo, 2017-09-29) Ramos, Ingrid Tigre; Gomes, Ana Maria Martins; Barroso, Danielle Resende Camisasca; Barros, Liliana Aparecida Pimenta de; Vaz, Sergio Lins de Azevedo; Velloso, Tânia Regina Grão
Introduction: The awareness of the dental characteristics associated with Down syndrome (DS) is fundamental to improve the quality of the dental service offered to these patients. Objective: Verify the types of systemic alterations present in children with DS; to investigate dental and jaw abnormalities, and the dental maturation stage in these children, comparing the results with a group of nonsyndromic children. Material and methods: The sample consisted of 93 panoramic radiographic exams, of which 31 were children with DS, who constituted the experimental group (EG), and 62 non-syndromic children that composed the control group (CG), aged 6-12 years. The variables studied were: systemic alterations (hypothyroidism, heart disease, alterations in airways, others), being evaluated only in the EG; dental anomalies (presence, absence, type and location), jaw abnormalities (presence, absence, type and location) and dental maturation stage, according to the Demirjian’s method. To verify the association of the variables, the chi-square statistical test was performed, considering values of p<0.05. Results: Out of the study population, 74% (n=23) of the EG presented some kind of systemic alteration (p<0.0001), being the most commonly found alterations in airways (45%; n=14). Dental anomalies were present in 74% (n=23) cases in EG and in 24% (n=16) in CG (p<0.0001), with a higher prevalence in EG for: hypodontia (p<0.0001), taurodontism (p=0.002) and microdontia (p=0.002). In EG, 69,6% (n=16) of dental anomalies were located in maxilla and mandible simultaneously (p <0.0001) and 65% (n=15) concomitantly in anterior and posterior regions (p=0.003). No jaw abnormalities were observed in EG, and in CG were observed in 6 (9%) patients (p=0.17). There was no statistically significant difference in the maturation stage between the groups. Conclusion: DS children have a high incidence of systemic alterations and, when compared to non-syndromic children, have a greater number of dental anomalies.
Estudo de biomarcadores relacionados à agressividade e invasividade em carcinoma de células escamosas oral
(Universidade Federal do Espírito Santo, 2015-11-20) Callegari, Eline Manhães Reid; Barros, Liliana Aparecida Pimenta de; Souza, Letícia Nogueira da Gama de; Pacheco, Marcos da Silva; Freitas, Vanessa Morais
In tumorigenesis process, laminin-332 performs multiple biologic effects by γ2 chain, and MMP-9 acts disrupting extracellular components. We aimed to assess the molecular profile of γ2 chain and MMP-9 in leukoplakias oral high-risk, carcinoma in situ (CIS) and oral invasive carcinoma cell squamous (OSCC) and establish possible associations with clinicopathologic features. γ2 chain and MMP-9 expression were assessed by immunohistochemistry in 10 patients with high-risk malignancy lesions and 26 cases of invasive OSCC. The immunostaining of γ2 chain in high-risk lesions showed most cases with a continuous basement membrane, on the other hand the majority of invasive OSCC had a discontinuous membrane or absence. OSCC stromal cells showed a higher presence of MMP-9 compared to cases of high-risk lesions. The association between clinical and expression profile demonstrated that smokers with invasive OSCC had a higher expression of γ2 chain in epithelium and MMP-9 in invasive fronts. A higher expression of MMP-9 in stromal cells was associated with male patients, older than 60 years and diagnosed with invasive OSCC when compared with high-risk lesions. Our results indicate an association between clinical and microscopic features in oral lesions with high malignant potential and oral carcinomas, with a progressive change in γ2 chain and MMP-9 expression during tumorigenesis.
Estudo in vitro da anatomia dos canais da raiz mésio-vestibular em primeiros molares superiores utilizando-se diferentes métodos de estudo
(Universidade Federal do Espírito Santo, 2009-06-19) Ferrari, Viviany Bertollo Cozer; Pereira, Rosana de Souza; Azeredo, Rogério Albuquerque; Liberti, Edson Aparecido; Barros, Liliana Aparecida Pimenta de
abstract
Invasão neoplásica perineural e vascular de carcinomas de células escamosas orais : estudo clinicopatológico e imunoistoquímico
(Universidade Federal do Espírito Santo, 2013-05-24) Cavalcante, Wanessa Siqueira; Souza, Letícia Nogueira da Gama de; Barros, Liliana Aparecida Pimenta de; Lourenço, Sílvia Vanessa
Oral squamous cell carcinoma (OSCC) is the most common oral cancer and occurs mainly in oral mucous membranes and oropharynx, prevailing in adults in range from 50 to 70 years old and it is associated with smoking and alcoholism. Aspects as perineural and vascular invasions of the OSCC have been associated with bad prognosis, increased recurrence rates, aggressive behavior, metastasis and low survival. The diagnosis is crucial for proper treatment and, even though it is based on their clinical and histopathological features, it may be improved through immunohistochemistry technique. The purpose of this retrospective study was to identify perineural and vascular invasions in OSCC lesions diagnosed by the Program for Prevention and Early Diagnosis of Oral Cancer and Oral Lesions at Dental School, UFES from 2004 to 2011. Overall, the records of 29 OSSC-diagnosed patients had been found and their clinical and demographic data were collected. In order to determine tumor grading and to identify invasive process, it were performed sections processed through the hematoxiline and eosin staining technique and immunohistochemistry with CD31 and CD34 antibodies to identify vessels and S100 and PGP9.5 antibodies to identify nerves. The obtained data were statistically assessed by qui-square test, Fisher’s exact test, maximum likelihood ration, Kappa test and McNemar’s non-parametric test. It was found 69% of cases with well differentiated tumor grade, significantly related to juxtaposed peritumoral inflammatory infiltrated. Vascular and perineural invasions were found in 17.2% and 31% of cases analyzed through H&E staining, respectively. There was intense peritumoral microvascular density in 82.8% of cases, as immunostaining with CD31. The CD34 antibody recorded 6.9% of cases with vascular invasion. Perineural neoplasic invasion was detected in 44.8% of cases immunostained with S100 protein and in 58.6% of cases immunostained with PGP9.5 protein. On agreement analysis between H&E and immunohistochemistry techniques, as regards presence of perineural invasion, the PGP9.5 protein was more effective than the others. It was concluded that the identification of vascular and perineural neoplasic invasions is a useful tool for the diagnosis and prognosis of patients with OSCC, might be used histopathological and immunohistochemistry methods, being the latter more effective for that analysis.
Uso tópico do alendronato de sódio associado ao bio-oss® no reparo de defeitos ósseos : estudo histomorfométrico em calvária de ratos
(Universidade Federal do Espírito Santo, 2015-12-04) Musso, Douglas Bertazo; Bertollo, Rossiene Motta; Silva, Daniela Nascimento; Barros, Liliana Aparecida Pimenta de; Zanetti, Liliane Scheidegger da Silva
Alendronate of sodium has shown the anabolic effect in bone healing when associated in the grafts from lyophilized bovine bone, but a form of administration and the concentration are still under investigation. Objective: This study evaluated the effect of topical of 0.5% ALN, used alone or in association with the lyophilized bovine bone (Bio-Oss®), on the percentage of bone formation and absorption of graft. Methodology: Two 5mm diameter cavities were made in the cranium of 18 Wistar rats and filled with 0.5% ALN, broadcasted in gelatin sponge (Group A – GA) Bio-Oss® (Group B – GB); Bio-Oss® soaked in 0.5% ALN (Group AB – GAB); blood clot (Control Group – GC). The animals were euthanized at 90 days postoperatively. The specimens were analyzed histologically (hematoxylin-eosin) and for histomorphometric analysis with the aid of AutoCAD® software, as the percentage of bone formation and remnants of Bio-Oss® granules. In the statistical analysis, the groups were compared using the “t” test of Student for paired samples (p<= 0.05). Results: The mean bone formation percentages were: GA = 40.4 ± 22.2%; GB = 46.0 ± 22.0%; GAB = 29.9 ± 17.8%; GC = 49.9 ± 16.4%, as long as the average of the percentages of the remaining granules of Bio-Oss was GB = 9.2± 4.5; GAB = 12.2 ± 7.2%. There was a significant statistical difference in the percentage of bone formation only between CG and GAB (p = 0.025), with the defect filled in without statistical difference as result of the presence of the granules of the graft. There was no significant statistical difference in the percentage of remaining granules between the groups. Conclusion: The topical use of ALN 0.5% isolated in the bone cavities did not change the percentage of bone formation. When combined with Bio-Oss®, decreased the percentage of bone formation and did not interfere in the reabsorption of the graft.

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