Doutorado em Ciências Fisiológicas
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Navegando Doutorado em Ciências Fisiológicas por Autor "Abreu, Glaucia Rodrigues de"
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- ItemCaracterização dos efeitos da euphorbia tirucalli sobre a função cardíaca de ratos normotenso(Universidade Federal do Espírito Santo, 2023-02-15) Barroso, Maria Eduarda de Souza; Meyrelles, Silvana dos Santos; https://orcid.org/0000-0003-0167-4093; http://lattes.cnpq.br/7731215198101947; https://orcid.org/0000-0002-7696-0919; http://lattes.cnpq.br/7624081067739433; Abreu, Glaucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; Baldo, Marcelo Perim; https://orcid.org/0000-0002-7673-3580; http://lattes.cnpq.br/7820422119282248; Pereira, Thiago de Melo Costa; https://orcid.org/0000-0003-4053-5061; http://lattes.cnpq.br/2027151219358701; Kuster, Ricardo Machado; https://orcid.org/0000000289615348; http://lattes.cnpq.br/4149814906786366There are many reports of people about using medicinal plants, ignore the existence of real scientifically proven effectiveness and many others end up abandoning allopathic treatments in the hope of healing through these plants. This behavior has become very common among patients diagnosed with cancer who, in search of a cure. The aveloz (Euphorbia tirucalli) it is a plant of African origin that is popularly used for various diseases, including the treatment of cancer. This latex has already presented several therapeutic actions such as antioxidant, immunomodulatory and even anticancer activity in some cell colonies. Most of the studies, however, were carried out in vitro, with a lack of in vivo studies to certify its properties. The aim of the present work was to characterize the actions of Euphorbia tirucalli on the cardiovascular system of wistar rats. For this, wistar rats were divided into 2 groups: control treated with water and treated with E. tirucalli at a dose of 13,06mg/Kg for 15 days by gavage. The animals were evaluated for blood pressure by tail plethysmography and functional aspects of the cardiovascular system such as contractility and relaxation were analyzed by cardiac hemodynamics. Tissue parameters such as cardiac remodeling and pulmonary congestion were also analyzed. The investigation of cellular parameters included the analysis of oxidative stress markers in the DNA of blood cells, bone marrow cells and heart cells, as well as biomolecular analyzes of oxidative stress in plasma. The percentage of apoptosis and possible necrosis was evaluated in cardiac cells from animals in both groups. The animals showed no difference between their weights and blood pressure before and after the treatments. Cardiac remodeling and pulmonary congestion indices did not differ significantly between groups. In the hemodynamic evaluation, the treated animals did not show changes in intraventricular pressure and heart rate, but a significant increase in the duration of the cardiac cycle can be observed in animals treated with E. tirucalli. In the evaluation of ventricular contractility (dp/dtmax + ) there was a significant increase in animals treated with E. tirucalli and a decrease in TAU, with no changes between groups in the dp/dtmax -. In the biochemical analyzes, a significant increase in AOPP can be observed (15,86±0,8µmol/L control group; 30,48±2,4µmol/L E. tirucalli group.) and Myeloperoxidade (0,051±0,006UA control group; 0,111±0,016UA E. tirucalli group) with this difference not being maintained between groups in the TBARS analysis. During the evaluation of reactive species in the isolated cells, an increase in the marking of DHE and DCF in the blood of the animals treated with speed 15 days can be observed, a result that is not repeated in the isolated cells of the bone marrow and only DCF demonstrated to be increased in cells heart of rats treated with E. tirucalli. No significant difference was demonstrated between the parameters and viability, apoptosis and necrosis evaluated in cardiac cells. The results indicate a possible harmful action of E. tirucalli on parameters of contractility and relaxation of the cardiac muscle and that this phenomenon probably involves oxidative stress. This implies the need for further investigations to better elucidate their mechanisms of action on a healthy organism.
- ItemEfeitos do ácido elágico sobre a reatividade vascular em artérias de resistentes de ratas hipertensas e ovariectomizadas(Universidade Federal do Espírito Santo, 2019-08-23) Silva, Fabricio Braganca da; Abreu, Glaucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; https://orcid.org/; http://lattes.cnpq.br/3532755171099760; Mill, Jose Geraldo; https://orcid.org/000000020987368X; http://lattes.cnpq.br/2497419234600362; Mauad, Helder; https://orcid.org/0000-0001-9694-2593; http://lattes.cnpq.br/4554702077415995; Brasil, Girlandia Alexandre; https://orcid.org/0000-0002-5455-7141; http://lattes.cnpq.br/1402295792093274; Padilha, Alessandra Simao; http://lattes.cnpq.br/7658998034219799The postmenopausal period is considered a milestone for the emergence of chronic noncommunicable diseases, including cardiovascular diseases. In this context hypertension and endothelial dysfunction form the basis of the main risk factors and injuries. The introduction of nutraceuticals as therapeutic alternatives has attracted the attention of researchers for presenting promising results. These include ellagic acid, a phytochemical with potent antioxidant properties found in fruits, plants, and nuts. Therefore, the objective of this research was to evaluate the effects of ellagic acid treatment on vascular reactivity of resistance arteries in an experimental model of hypertension and menopause. Therefore 40 SHR rats were evaluated for 4 weeks and separated into 5 groups according to treatment: vehicle-treated SHAM (carboxymethylcellulose); OVX, castrated and vehicle treated; OAE, castrated and treated with ellagic acid (10 mg / kg / day); OE2, castrated and treated with estrogen (1g / kg / day); OAE + E2, treated with ellagic acid and estrogen. Mesenteric artery rings were maintained in vitro and subjected to contraction strength tests in the absence and presence of the nitric oxide synthase enzyme inhibitor to record the effect of treatments and the participation of nitric oxide (NO). Vessel samples were taken for biochemical analysis. Castration produced an increase in blood pressure (MAP: 190.4 ± 4.4 mmHg); effect that did not occur in groups treated with ellagic acid (MAP: 165.7 ± 1.4 mmHg, p
- ItemEfeitos dos diferentes tempos de início de treinamento físico após o infarto do miocárdio sobre a função cardíaca e o remodelamento ventricular esquerdo em ratas ovariectomizadas(Universidade Federal do Espírito Santo, 2017-05-12) Claudio, Erick Roberto Gonçalves; Abreu, Glaucia Rodrigues de; Leite, Richard Diego; Gouvêa, Sônia Alves; Futuro Neto, Henrique de Azevedo; Mauad, HelderThe aim of the present study was to evaluate the effects of the different time of exercise training (ET) initiation after myocardial infarction (MI) on the cardiac function and the ventricular remodeling in ovariectomized rats (OVX). Twelve-week-old OVX female Wistar rats (Rattus Norvegicus Albinus) were randomly divided into four groups: MI-SHAM (SHAM), MI sedentary (MI), MI which started ET 3 days after MI (MI3+ET) and MI which started ET 15 days after MI (MI15+ET). Low to moderate intensity training protocol in a motorized treadmill was performed during 8 weeks (60 min/d, 5 days/wk). Forty-eight hours after the last training session, animals were anesthetized and subject to evaluation of the following parameters: i) Cardiac function after a catheter insertion in the left ventricle (LV); ii) MI area; iii) collagen deposition and myocyte hypertrophy by histology; iv) myocardial oxidative stress by the “in situ” generation of superoxide (DHE) and by the evaluation of the advanced oxidation protein products (AOPP); v) protein expression of type 1 receptor of angiotensin II (AT-1R) and of the membrane isoform of multi-enzymatic complex of NADPH oxidase (gp91phox) by the western blotting (WB) method; vi) antioxidants protein expression, such as superoxide dismutase (SOD-1 and SOD-2) and catalase (CAT), also by WB; vii) antioxidant enzymes activities (SOD and CAT).
- ItemEficácia dos exercícios de alongamento e de estabilização lombar no tratamentro de lombalgia crônica(Universidade Federal do Espírito Santo, 2010-08-03) Maria Angelica Ferreira Leal Puppin; Marques, Amélia Pasqual; Futuro Neto, Henrique de Azevedo; Ferreira , Elizabeth Alves Gonçalves; Abreu, Glaucia Rodrigues de; Araújo , Maria Teresa Martins deLow back pain affects over 70% of the population, mostly economically active adults. In addition to the high incidence, chronicity and disability makes this disorder a public health problem in industrialized countries. Kinesitherapy is the first line of choice in physical therapy, but there is no evidence of which type of exercise is more effective. The aim of this study, was to assess, the effectiveness of the GDS method on pain, functional disability, global flexibility and ability to contract the transverse muscle of abdomen (TrA) in individuals with chronic low back pain. 82 patients were randomized into three groups: Stretching (n = 30, age 37.5 ± 12.1) subjected to stretching exercises; Stabilization (n = 27, age 39.0 ± 12) which performed exercises to recruit the deep muscles of the lower trunk and Control (n = 25, age 37.8 ± 13.6) that were not treated. The intervention groups were treated with two weekly sessions lasting 40 minutes, for eight weeks. Pain was assessed by visual analogue scale; functional disability by the Oswestry Index, global flexibility by the third finger to the ground and the ability of TrA contraction by the pressure biofeedback unit. The variables were analyzed before, after and after eight weeks of treatment. We used the single-factor ANOVA with repeated measures and post hoc Holm-Sidak for parametric variables and Friedman's test and Tukey for non parametric at comparisons within and between groups. A significance level of 5% was employed. We also evaluated the relative clinical gain. The results showed significant improvement in pain, functional disability and overall flexibility after treatment and after eight weeks (p <0.05) in the intervention groups. Only the stabilization group was effective in improving the capacity of contraction of TrA (p <0.05). When comparing groups, the two forms of intervention were effective in reducing pain and functional disability (p <0.05). Nevertheless only the Stretching group showed significant improvement in global flexibility (p = 0.01) There was no differences between groups after eight weeks of treatment in functional disability (p = 0.10) and global flexibility (p = 0.07). There was no difference between groups in the ability of contraction of TrA in any of the times evaluated. The two experimental groups had gains on clinical relevance, especially the Stabilization group, except for global flexibility variable in which the stretching exercises had higher gains. The two treatments, stretching and lumbar stabilization used in GDS were effective in reducing pain, functional disability and improving global flexibility, but only the stabilization exercises showed improved ability of the contraction of the TrA in patients with chronic low back pain.
- ItemIMPACTO DO TRATAMENTO COM OXANDROLONA NAS FUNÇÕES CARDÍACA E RENAL DE ANIMAIS JOVENS(Universidade Federal do Espírito Santo, 2022-03-09) Ronchi, Silas Nascimento; Bissoli, Nazare Souza; https://orcid.org/; http://lattes.cnpq.br/8865368585732583; https://orcid.org/; http://lattes.cnpq.br/; Abreu, Glaucia Rodrigues de; https://orcid.org/; http://lattes.cnpq.br/0229590907405570; Meyrelles, Silvana dos Santos; https://orcid.org/; http://lattes.cnpq.br/7731215198101947; Brasil, Girlandia Alexandre; https://orcid.org/; http://lattes.cnpq.br/; Garcia, Ana Raquel Santos de MedeirosOxandrolone (OXA), a synthetic analogue of testosterone, is used in clinical practice because most of its effects are anabolic and low androgenic, so its abuse for aesthetic purposes has spread. In view of this problem and the scarce literature on the eff
- ItemImplicações da terapia estrogênica associada ao exercício físico sobre a função cardíaca de ratas infartadas e ovariectomizadas(Universidade Federal do Espírito Santo, 2019-02-15) Simoes, Simone Alves de Almeida; Abreu, Glaucia Rodrigues de; https://orcid.org/0009-0008-8772-8470; http://lattes.cnpq.br/0229590907405570; https://orcid.org/; http://lattes.cnpq.br/4036865618615755; Neto, Henrique de Azevedo Futuro; https://orcid.org/0000-0001-6887-5290; http://lattes.cnpq.br/2687624857190195; Mauad, Helder; https://orcid.org/0000-0001-9694-2593; http://lattes.cnpq.br/4554702077415995 ; Bissoli, Nazare Souza; https://orcid.org/0000-0002-3456-2437; http://lattes.cnpq.br/8865368585732583; Leopoldo, Andre Soares; https://orcid.org/0000000231312467; http://lattes.cnpq.br/5912424547697713There is an increase in the incidence of cardiovascular events such as myocardial infarction (MI) after menopause. However, the use of estrogen therapy (E2) remains controversial. The aim of this study was to evaluate the effects of E2, alone and combined with exercise training (ET), on cardiac function and remodeling in ovariectomized (OVX) rats after MI. Wistar female rats underwent ovariectomy, followed by MI induction were separated into five groups: S; MI; MI+ET; MI+E2; and MI+ET+E2. Fifteen days after MI or sham surgery, treadmill ET and/or estrogen therapy [17-β estradiol-3-benzoate (E2), s.c. three times/week] were initiated and maintained for 8 weeks. After the treatment and/or training period, the animals underwent cardiac hemodynamic evaluation through catheterization of the left ventricle (LV); the LV systolic and diastolic pressures (LVSP and LVEDP, respectively), maximum LV contraction and relaxation derivatives (dP/dt+ and dP/dt−), and isovolumic relaxation time (Tau) were assessed. Moreover, histological analyses of the heart (collagen and hypertrophy), cardiac oxidative stress [advanced oxidation protein products (AOPPs)], pro- and antioxidant protein expression by Western blotting and antioxidant enzyme activity in the heart were evaluated. The MI reduced the LVSP, dP/dt+and dP/dt−but increased the LVEDP and Tau. E2 did not prevent the MI-induced changes in cardiac function, even when combined with ET. An increase in the dP/dt+ was observed in the E2 group compared with the MI group. There were no changes in collagen deposition and myocyte hypertrophy caused by the treatments. The increases in AOPP, gp91-phox, and angiotensin II type 1 receptor expression induced by MI were not reduced by E2. There were no changes in the expression of catalase caused by MI or by the treatments, although, a reduction in superoxide dismutase (SOD) expression occurred in the groups subjected to E2 treatment. Whereas there were post-MI reductions in activities of SOD and catalase enzymes, only that of SOD was prevented by ET. Therefore, we conclude that E2 therapy does not prevent the MI induced changes in cardiac function and worsens parameters related to cardiac remodeling. Moreover, E2 reverses the positive effects of ET when used in combination, in OVX infarcted female rats.