Doutorado em Ciências Fisiológicas

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Ação moduladora da tibolona no sistema de peptídeos natriuréticos: Implicações cardiovasculares e imunológicas
(Universidade Federal do Espírito Santo, 2010-11-24) Garcia, Ana Raquel Santos de Medeiros; Andrade, Tadeu Uggere de; Bissoli, Nazaré Souza; Reis, Adelina Martha dos; Boechät, Giovanna Assis Pereira; Padilha, Alessandra Simão
Cardiovascular and immune system abnormalities have been reported in females with estrogen deficiency. Nevertheless, the capacity of the exogenous estrogen therapy to manage these hazard effects in postmenopausal women is still to be better investigated. Objective: this study is addressed to determine the abnormalities on the natriuretic peptide system in ovariectomized rats with and without tibolone reposition therapy, and if these changes are or not related with cardiovascular and immunological parameters. Materials and Methods: female rats with the average weight of 160-180 g were used and divided into four groups (n = 7 per group): SHAM, ovariectomized (OVX), OVX treated with 17β-estradiol (EST) and OVX treated with tibolone (TIB). The treatment period was 14 days with TIB (1.5 mg / kg / day) and EST (0.5 mg / kg / day), after 21 days of the ovariectomy procedure. At the end of treatment, the animals were euthanized, the thoracic aorta was isolated, and rings of 4-5 mm were removed. Rings with endothelium, were placed in an isolated organ tank with Krebs solution. Dose-response curves to phenylephrine (PHE) and acetylcholine (ACH) were obtained for assessment of vascular reactivity. Upper segment of the thoracic aorta was used for analysis of protein expression of eNOS by Western Blotting. The kidneys, the atria and blood were collected for further analysis. Plasma levels of atrial natriuretic peptide (ANP) were measured by radioimmunoassay (RIA) and serum cytokines (IL-6 and TNF-α) by ELISA. Atria were removed for analysis by RIA of ANP and atrial ANP mRNA by RT-PCR. The kidneys were used for analysis of mRNA of type A receptor (NPR-A) and C (NPR-C) by RT-PCR. Data were presented as mean ± standard error of mean. Statistical analysis of the reactivity of aortic rings was performed by analysis of variance (ANOVA), and one or two way, followed by post-hoc test of Tukey. For the peptides system data it was performed one-way analysis of variance (ANOVA) followed by post hoc Newman-Keuls, and for eNOS it was used one-way analysis of variance (ANOVA) followed by Fisher's post hoc test. Significance levels were p<0,05. Results: the OVX group showed increase of contractile response to PHE compared to the SHAM group, and the TIB and EST groups were able to normalize this response (Emax - SHAM: 91,0 ± 3,9; OVX: 140,0 ± 5,5; EST: 115,4 ± 3,7; TIB: 113,2 ± 5,1 - % Tension). The endothelium-dependent relaxation (dose-response curves to ACH) was similar for all groups. The OVX, EST and TIB groups showed no difference in the eNOS protein expression compared with the SHAM group, however, serum levels of IL-6 and TNF-α were increased in ovariectomized females. However, the treatments with EST and TIB were able to decrease these levels compared to the SHAM group, except the levels of IL-6 which remained high in the TIB group (TNF-α: SHAM 20.3 ± 2.0, OVX 31.0 ± 5.0, 17.6 ± EST 2.0; TIB: 20.5 ± 3.1 / IL-6: SHAM 19.3 ± 6.0, 32.8 ± 5.0 OVX, EST 20.0 ± 3.0; TIB: 34.4 ± 2.8 pg / ml). The plasmatic and left atrial levels of ANP (ANP plasma: 263.3 ± 53.3 SHAM, OVX 92.5 ± 19.5, 247.3 ± 29.9 EST; TIB: 285.7 ± 60.4 pg / ml / left atrial ANP: SHAM 5.68 ± 0.35; OVX 3.48 ± 0.40, 0.97 ± 6:48 EST; TIB: 3.59 ± 0.25 mg / mg protein), the mRNA expression of ANP left atrial (SHAM 91.3 ± 4.4, OVX 40.0 ± 10.1; 122.3 ± 12.0 EST; TIB: 167.9 ± 25.7 AU) and NPR-A (SHAM 5.20 ± 12:00; OVX 0.68 ± 0.11; EST 0.92 ± 0.28; TIB: 2.04 ± 1.4 AU) were depressed after ovariectomy, and NPRC did not change. The treatment normalized these parameters, except that the TIB group did not normalize the levels of atrial ANP. Conclusion: data from this study show, for the first time, that tibolone treatment after ovariectomy influence the natriuretic peptide system and the inflammatory cytokines. Tibolone was able to normalize the levels of ANP and its atrial expression as well as reduce levels of TNF-α. Additionally, the results show that Hormonal Therapy used in this study partially normalized the changes in vascular reactivity, possibly through regulation of the peptide system and inflammatory cytokines.
Avaliação de marcadores de remodelamento cardíaco (funcionais, bioquímicos e estruturais) em ratos submetidos a exercício resistido e tratamento com decanoato de nandrolona.
(Universidade Federal do Espírito Santo, 2015-07-13) Lima, Ewelyne Miranda de; Orientador1; https://orcid.org/; http://lattes.cnpq.br/; Andrade, Tadeu Uggere de; Sampaio, Karla Nívea; Gouvêa, Sônia Alves; Campagnaro, Bianca Prandi
Resistance training (RT) has often been associated with the abuse of anabolic androgenic steroids (AAS) by athletes and non-athletes. The aim of this study was to evaluate the effects of resistance training (RT) with or without nandrolone decanoate, with the levels of cytokines and activity of angiotensin-converting enzyme (ACE) in the heart, and the sensitivity of cardiopulmonary Bezold-Jarisch reflex (BJR). Male Wistar rats were divided into 3 groups: C (receiving vehicle, untrained); E (RT: after one week of adaptation water, rats were trained to jump into the water twice a week for 4 weeks), DN (treated with nandrolone decanoate 10 mg kg twice a week, intramuscularly) e DNE (treated with nandrolone decanoate 10 mg kg twice a week, intramuscularly, associated with RT). The BJR was analyzed by measuring bradycardic and hypotensive responses induced by administration in bolus of five serotonin randomized doses (2 to 32 g/kg body weight). Myocyte hypertrophy and collagen deposition in the matrix was determined by morphometric analysis of images from slides stained with H&E and picrosirius red respectively. The TNF-α levels in the heart and ACE activity was determined by Elisa assay. The RT promoted resting bradycardia in E group (bpm 312 ± 9, p <0.01) when compared with C group (360 ± 11 bpm) and ND did not influence this parameter NDE (bpm 324 ± 9, p <0.05), furthermore promoted physiological hypertrophy in myocytes without collagen deposition and no other changes were observed. The ND association with RT shown myocyte hypertrophy, type I collagen deposition, increase on TNF-α levels (C: 207 ± 9; E: 211 ± 8; DN: 269 ± 10; DNE: 242 ± 10 pg / mg, p <0,01) and ACE activity(C: 27,0 ± 7 ; E: 39,0 ± 6; DN: 50 ± 5; DNE: 65,0 ± 4 %, p <0,01), decreased IL-10 levels (C: 220 ± 7; E: 231 ± 5; DN: 165 ± 5; DNE: 164 ± 4 pg / mg, p<0,01), impairment in the sensitivity of BJR and hypertension(C: 104 ± 2 mmHg; E: 108 ± 8 mmHg; DN: 144 ± 7; DNE: 138 ± 5 mmHg; p <0,01). ND is associated with changes in cardiac structure and function as a result of the development of pathological cardiac hypertrophy (cytokine imbalance in heart elevation of ACE activity) and cardiac injury, and resistance training was unable to reverse the deleterious cardiovascular effects.
Avaliação dos efeitos do tratamento com sildenafil na hipertensão induzida por infusão de angiotensina II
(Universidade Federal do Espírito Santo, 2018-11-09) Moreira, Ananda Tissianel Dias; Vasquez, Elisardo C; Gouveia, Sônia Alves; Padilha, Alessandra Simão; Oliveira, Jairo Pinto de; Andrade, Tadeu Uggere de
Angiotensin II (Ang II) is responsible for multiple actions in the organism, being considered an effector peptide of several of physiological and physiopathological actions in the Renin-Angiotensin-Aldosterone (RAA) system. This endogenous peptide shows an important role in development of arterial hypertension (AH), since it is capable to promote deleterious effects target organs of AH, such as morphofunctional changes in arteries and heart. This study aimed to test the hypothesis that an inhibitor of phosphodiesterase 5 (PDE5), sildenafil, can reducing the damage caused by the increase of Ang II. Therefore, we evaluated the effects of the treatment on blood pressure (BP), cardiac hypertrophy, Ang II plasma levels, morphofunctional changes of the arteries of conductance and resistance, and the systemic and vascular cells oxidative stress. Male mice wild-type C57BL/6 were divided into the following groups: Normotensive (control) and hypertensive animals. Mice were induced to hypertension by Ang II infusion (1000 ng/kg/min, for 4 Weeks). Thereafter, we treated the groups with Sildenafil (40 mg/kg/day) or water for the last two weeks. Temporal analysis of systolic BP (SBP) was performed throughout the experimental period (prior to infusion and 7, 14, 21 and 28 days after the infusion of Ang II). At the end of the treatment the animals were anesthetized and the blood was collected for the plasma quantification of Ang II and the heart was removed to verify the cardiac hypertrophy. Mesenteric arterial bed (MAB) and thoracic aorta were removed for analyses of vascular function, morphology and oxidative stress. We found that, after 4 weeks, the hypertensive animals showed an increase of SBP (186 ± 3 mmHg), of plasma levels of Ang II (53 ± 2.4 pg/mL) and the index of cardiac hypertrophy (0.09 ± 0.003 mg/mm). The treatment with sildenafil was able to reduce the SBP in 23 mmHg and the Ang II plasma levels and cardiac hypertrophy showed a decreasing of 30% and 32%, respectively. Vascular function analyses revealed an increased contractile response in hypertensive animals (Rmax: 70%) in both vessels of the study, presenting reactivity norepinephrine in MAB and phenylephrine in aortic rings. The treatment with sildenafil was capable to restore the endothelial function, reducing the reactivity to sympathomimetics drugs. Part of these effects are mainly caused by the increasing the nitric oxide (NO) and the decreasing of reactive oxygen species (ROS) and prostanoids vasoconstrictors. In addition to the morphofunctional changes, Ang II induced the vascular hypertrophy and promoted alteration in the endothelial surface. Treatment with sildenafil promoted 12 important morphological changes, such as reduction of the cross-sectional area and a restructuring of the endothelial cells. Superoxide anion, peroxynitrite and hydroxyl radical analyses revealed that Ang II increase in these ROS and the treatment with sildenafil was able to reduce oxidative stress. Taken together, our results demonstrate that sildenafil decreased the deleterious effects of Ang II on the resistance and conductance vessels, mainly by promoting a balance between ROS/NO/Prostanoids vasoconstrictors and vasodilators. This study represents an advance in the beneficial effects of sildenafil. The data present herein may be useful for new clinical studies for cardiovascular diseases treatment associated with intense activation of RAA system and for the hypertension resistant treatment.
Dimorfismo Sexual na Hipertrofia Ventricular Esquerda e Função cardíaca de Ratos Espontaneamente Hipertensos: O Papel da Atividade da Enzima Conversora de Angiotensina (ECA) e ECA2 no Ventrículo Esquerdo e no Rim
(Universidade Federal do Espírito Santo, 2015-09-01) Dalpiaz, Polyana Lima Meireles; Orientador1; https://orcid.org/; http://lattes.cnpq.br/; Baldo, Marcelo Perim; Gouvea, Sonia Alves; Andrade, Tadeu Uggere de; Futuro Neto, Henrique de Azevedo
Abstract There is growing interest in sex differences and RAS components; however, whether gender influences cardiac angiotensin I-converting enzyme (ACE) and angiotensin-converting enzyme 2 (ACE2) activity is still unknown. In the present work we determined the relationship between ACE and ACE2 activity on kidney and heart, left ventricular function and gender in spontaneously hypertensive rats (SHRs). Twelve-week-old female (F) and male (M) SHRs were divided into 2 experimental groups (n = 7 in each group): sham (S) and gonadectomized (G). Fifty days after gonadectomy, we measured positive and negative first derivatives (dP/dt+ and dP/dt-), hypertrophy (morphometric analysis) and ACE and ACE2 catalytic activity in kidney and LV (left ventricle). Expression of calcium handling proteins and AT1 receptor was measured by western blot analysis. Male rats exhibited higher cardiac ACE and ACE2 activity as well as hypertrophy compared to female rats. Orchiectomy reduced ACE activity and hypertrophy, while ovariectomy increased these variables. In relation to cardiac function, the sham group, male to compared female rats, had a lower +dP/dt (MS: 5140 ± 259 mmHg vs. FS: 6485 ± 178 mmHg) and lower -dP/dt (MS: -3.717 ± 129 mmHg vs. FS: -5.250 ± 115 mmHg) than the female sham group. After gonadectomy, the results inverted such that they were increased in males and reduced in female rats in dP/dt+ (GM = 6.728 ± 177 mmHg vs FG: 5453 ± 229 mmHg) and dP/dt- (SM: -4.851 ± 290 mmHg vs GF = -3.913 ± 136 mmHg). No difference among the groups in the SERCA2a protein expression was observed. The gonadectomy increased protein expression of PLB and the PLB to SERCA2a ratio in female rats, but did not alter in male rats. In conclusion, the ovariectomy leads to increased cardiac hypertrophy, ACE2 activity, PLB expression and PLB to SERCA2a ratio, and worsening of hemodynamic variables, wher-eas in males the removal of testosterone has the opposite effect in RAS components. Our data suggest that sex steroids are of great importance for both female and male SHRs in influencing cardiac function and in regulating the RAS in kidney and LV. In our study, in LV the withdrawal of estrogen in females led to increased cardiac hypertrophy, increased ACE2 activity, PLB expression and PLB to SERCA2a ratio, what could be related to worsening of hemodynamic variables. In males, however, the removal of testosterone had the opposite effect in RAS components. The increase in ACE2 activity after ovariectomy and the reduction after orchiectomy seems to be occurring to counterbalance the observed changes. In the kidney, in females the activity of ACE and ACE2 was higher, favoring the balance between these two important components of the RAS. In the male, conversely, lower levels of ACE and ACE2 favoring the imbalance. ACE, although important, has not changed with the hormonal status, so that in this model, the ACE2 have greater involvement in local modulation of these components. Certainly, the assessment of sexual dimorphism of the components of the RAS can help the continued development of effective therapeutic interventions for cardiovascular diseases.
Efeitos da Metformina e de ácidos graxos Ômega-3 sobre parâmetros autonômicos e vasculares em ratos hipertensos
(Universidade Federal do Espírito Santo, 2018-05-18) Oliveira, Phablo Wendell Costalonga; Bissoli, Nazaré Souza; Baldo, Marcelo Perim; Santos, Roger Lyrio dos; Andrade, Tadeu Uggere de; Gouvea, Sonia Alves; Figueiredo, Suely Gomes de
abstract
Efeitos do estresse oxidativo em células de medula óssea, sangue e aorta de camundongos apoe knockout" por "Aterosclerose e envelhecimento- Efeitos do estresse oxidativo em células de medula óssea, sangue e aorta
(Universidade Federal do Espírito Santo, 2013-03-27) Tonini, Clarisse Loureiro; Orientador1; https://orcid.org/; http://lattes.cnpq.br/; Meyrelles, Silvana dos Santos; Endringer, Denise Coutinho; Bissoli, Nazare Souza; Andrade, Tadeu Uggere de; Vasquez, Elisardo Corral
The relationship between production of reactive oxygen species and the development of atherosclerosis has been studied for decades. It is known that all inflammatory processes involved in plaque progression leads to increased production of free radicals. Furthermore, aging is a factor that aggravates atherosclerotic disease and also reactive oxygen species production, probably by the loss of antioxidant capacity that makes regular balancing of reactive oxygen species. The Apolipoprotein E deficient mouse is a model widely used in the study of atherosclerosis, in which there is high hypercholesterolemia that results in increased production of reactive oxygen species in the bone marrow, blood and aorta cells. Aging in turn also leads to increased production of reactive oxygen species, however when combined with hypercholesterolemia, the result of this phenomenon is the occurrence of increased production of reactive oxygen species and thus high levels of oxidative DNA fragmentation and apoptosis. In this regard, most notably is the analysis of bone marrow cells, a source of blood and stem cells for all organism that commonly presents a low oxygen tension ambient, in which we showed high generation of superoxide radicals and hydrogen peroxide. Thus, bone marrow is affected by oxidative imbalance caused by aging and atherosclerosis. Still, corroborating these data, in the analysis of DNA fragmentation by comet assay, bone marrow cells showed high levels of fragmentation in atherosclerotic and aged animals. Still, blood and aorta cells from atherosclerotic and aged animals also showed high superoxide anion production. However, there are low hydrogen peroxide production in endothelial cells of all groups, suggesting a possible modulation of tissue-specific effects of antioxidant enzymes. Furthermore, we note that there is not increased production of hydrogen peroxide in blood cells from aged C57 animals, unlike bone marrow mononuclear cells, in which we observed an increase. Finally, it can be noted that, in general, atherosclerosis increases the production of reactive oxygen species as well as aging, but when these two parameters are combined oxidative stress is further increased. The analysis of scientific literature suggests a possible activation of NADPH oxidase enzyme, responsible for the increased production of reactive oxygen species, and downregulation of antioxidant enzymes, which affect the balance of oxidative stress in tissues.
Nandrolona determina desequilíbrio das citocinas Inflamatórias e aumento da atividade da ECA cardíaca, acarretando remodelamento e injúria cardíaca, redução da sensibilidade do RBJ e desenvolvimento de hipertensão
(Universidade Federal do Espírito Santo, 2012-12-20) Franquini, João Vicente Maggioni; Andrade, Tadeu Uggere de; Bissoli , Nazaré Souza; Lunz, Wellington; Gouvêa, Sônia Alves
abstract
OSTEOPOROSE EM MENOPAUSA EXPERIMENTAL: PAPEL DO EXERCÍCIO FÍSICO OU DO TRATAMENTO COM TELMISARTANA
(Universidade Federal do Espírito Santo, 2019-11-19) Birocale, Antonio Marcos; Bissoli, Nazare Souza; https://orcid.org/; http://lattes.cnpq.br/8865368585732583; https://orcid.org/; http://lattes.cnpq.br/; Andrade, Tadeu Uggere de; https://orcid.org/; http://lattes.cnpq.br/; Sartorio, Carmem Luiza; https://orcid.org/; http://lattes.cnpq.br/1299417616233163; Padilha, Alessandra Simao; https://orcid.org/; http://lattes.cnpq.br/7658998034219799; Nogueira, Breno Valentim; https://orcid.org/0000000221990635; http://lattes.cnpq.br/0011229320439147
Telmisartan is a type I angiotensin receptor blocker and a partial PPARγ agonist that have been used to treat hypertension. PPARγ activation is known to act inducing boné loss. The aim of the present study was to evaluate two distinct interventi
Tratamento crônico com Decanoato de Nandrolona induz remodelamento cardíaco e renal em ratas, sem modificar parâmetros fisiológicos: o papel do sistema de ANP
(Universidade Federal do Espírito Santo, 2016-06-03) Brasil, Girlandia Alexandre; Andrade, Tadeu Uggere de; Bissoli, Nazaré Souza; Gouvêa, Sônia Alves; Campagnaro, Bianca Prandi; Endringer, Denise Coutinho
Anabolic Androgenic Steroids (AAS) are drugs used in an illicit manner, with mostly aesthetic proposes, by both men and women. Due the few researches about the action of these hormones in female cardiovascular system, the aim of the present study was evaluate the effect of chronic treatment with nandrolone decanoate (ND), in high dose in natriuretic peptide system of female rats submitted or not to physical activity and which is the influence of estrogen withdraw for the treatment. For that the animals were randomly divided into 8 groups (n=10 each): SHAM and OVX, were treated with vehicle (peanut oil) twice a week and were not submitted to physical exercise. SE and OVXE, were treated with vehicle and were submitted to resistive physical exercise (5 times a week); SD and OVXD, treated with ND (20mg/kg/week, divided into 2 doses) and were not exercised, and the groups SDE and OVXDE, which were treated and exercised. The OVX animals were submitted to bilateral ovariectomy and the treatments started 21 days after the surgery and were maintained for 4 weeks. After that period, the Bezold-Jarisch reflex (BJR) sensitivity was evaluated by random doses of phenylbiguanide administration. The animals were maintained into metabolic cages for 24h urine collection and after that creatinine, urea and sodium excretion were evaluated. The heart and kidney were maintained in formalin buffer for evaluation of hypertrophy (H/E) and collagen deposition (Picrossirius red) by histology. The right atrium and kidney were frozen in liquid nitrogen for evaluation of genic expression of ANP and NPR-A and NPR-C expression by RT-PCR. After treatment with ND the animals presented increase on genic expression of ANP on atrium and decrease of NPR-A on kidney. The NPR-C shows reduction on the groups that was treated with ND and exercised (SDE and OVXDE).besides that, the treatment promotes hypertrophy and collagen deposition on heart and kidney. Additionally the animals presented muscle hypertrophy (gastrocnemius muscle) and improve on athletic performance observed by increase on jumps/minute on ND treated groups. The treatment decrease estrogen concentration on Sham groups and increase on plasmatic level of testosterone. It was not possible observe change on mean arterial pressure, BJR sensitivity and sodium, creatinine and urea excretion. The ovariectomy little changes the pattern of response to treatment. Thus, we can observe that the treatment with ND changed the ANP system and that result could promote cardio protective effect, observed by not increase on mean arterial pressure and BJR sensitivity.
Tratamento crônico com decanoato de nandrolona prejudica o relaxamento vascular em ratas sedentárias e submetidas a treinamento físico
(Universidade Federal do Espírito Santo, 2017-03-31) Caliman, Izabela Facco; Bissoli, Nazaré Souza; Baldo, Marcelo Perim; Andrade, Tadeu Uggere de; Santos, Roger Lyrio dos; Figueiredo, Suely Gomes de
Nandrolone Decanoate (ND) is an Anabolic Androgenic Steroid (AAS) that under abusive regimen can lead to multiple physiological adverse effects. Studies of AASmediated cardiovascular (CV) alterations were mostly taken from male subjects, even though women are also susceptible to the effects of AAS and sex-specific differences in susceptibility to vascular diseases exist. The aim of this study was to investigate NDinduced vascular reactivity alterations in both sedentary and exercised female rats as well as the participation of endothelium-derived factors on the vascular response. Adult Wistar female rats (180-200g), sedentary or exercise trained, intact or ovariectomized, treated with ND (20 mg/kg/week) for 4 weeks were used. At the end of the experimental protocol, dose-response curves to acetylcholine (ACh) were performed in isolated mesenteric vascular beds (MVBs) in the absence and presence of pharmacological inhibitors to assess the vascular reactivity. Our main findings show that chronic exposure of female rats to ND impaired the endothelial function in MVBs from both sedentary and trained animals. The nitric oxide (NO) pathway appear to be the main responsible mechanism involved in the impairment of the vascular reactivity, as observed in the ACh-induced vasodilation in presence of L-NAME (NO synthase inhibitor (NOS) and aminoguanidine (selective inhibitor of inducible NOS), as well as by the decreasing of the phosphorylation sites of eNOS (Ser1177) and Akt (Ser473) and upregulation of iNOS and NADPH oxidase expression. Neither the endotheliumderived hyperpolarizing factor (EDHF) component nor prostanoids were altered in the MVBs from ND-treated females, since in the presence of L-NAME and Indomethacin (cyclooxygenase inhibitor) the vasodilator response to ACh remained unchanged amongst the studied groups.
Tratamento crônico com decanoato de nandrolona promove remodelamento cardíaco e alteração da função ventricular esquerda em ratas: papel dos moduladores intracelulares de Ca2+
(Universidade Federal do Espírito Santo, 2017-03-16) Nascimento, Andrews Marques do; Andrade, Tadeu Uggere de; Bissoli, Nazaré Souza; Endringer, Denise Coutinho; Graceli, Jones Bernardes; Ribeiro Junior, Rogerio Faustino
The use of androgenic anabolic steroids (AAS) has grown considerably in the last decades, being used by both men and women, with a significant reduction in the age of these users. The abusive and indiscriminate use of these hormones causes cardiovascular alterations, such as cardiac hypertrophy associated with collagen deposition considered pathological. This cardiac remodeling caused by AAS can trigger hypertension, congestive heart failure, cardiomyopathy, arrhythmias and even sudden death. Usually, AAS are associate with physical exercise, which alone promotes beneficial cardiovascular effects. However, in combination, the beneficial effect of exercise is lost with the occurrence of unfavorable cardiovascular adaptations and ventricular function problems, especially diastolic function, myocardial fibrosis and cardiomyocyte derangement. In addition, little is known about the cardiovascular effects of these drugs on women. This work aims to evaluate the effects of nandrolone decanoate and resistive physical exercise on cardiac contractility in female rats. The animals were separated into 4 groups: C (untrained); EC (submitted to resistive physical exercise in water, 5 times per week); ND (treated with DN, 20 mg/kg/ week for 4 weeks); and NDE (trained and treated). The hemodynamic parameters (+dP/dtmax, -dP/dtmin and Tau) were evaluated in the left ventricle. The heart was collected for histological analysis (H&E) and deposition of collagen (picrusirius red).
Valor prognóstico dos biomarcadores cardíacos e inflamatórios no câncer de cabeça e pescoço associados à terapêutica oncológica
(Universidade Federal do Espírito Santo, 2018-06-25) Oliveira, Karine Gadioli de; Bissoli, Nazaré Souza; Gouvêa, Sônia Alves; Palacios, Ester Miyuki Nakamura; Andrade, Tadeu Uggere de; Futuro Neto, Henrique de Azevedo; Sartorio, Carmem Luiza
Currently, cancer is the second largest cause of death in Brazil, surpassed only by cardiovascular diseases. Oncologic therapy has evolved and improved the prognosis in cancer patients. However, despite the unquestionable clinical benefit of this type of therapy, many of these drugs can cause undesirable side effects in the cardiovascular system. Head and neck cancer is the sixth most common cancer in the world and the prognosis for these types of patients is poor and has not improved in the last decades. Head and neck cancer patients have a high risk of non-cancer related mortality and one of the most frequent causes is cardiovascular disease. Therefore, the aim of this study was to evaluate the predictive value of C Reactive Protein (CRP), N-terminal pro–Btype natriuretic peptide (NT-proBNP), cardiac troponin T (cTnT), and cytokines (IL-17A, INF-γ, TNF-α, IL-10, IL-6, IL-4) in cardiovascular risk associated with oncologic therapy in head and neck squamous cell carcinoma. Thus, 118 patients with confirmed diagnosis of head and neck squamous cell carcinoma have been included in this study, and clinical-epidemiological data have been obtained with blood collection before and after cancer therapy.

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