Mestrado em Biotecnologia
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Navegando Mestrado em Biotecnologia por Autor "Arantes, Lidia Maria Rebolho Batista"
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- ItemAnálise da relação entre depressão e hábito tabagista na metilação do gene BDNF(Universidade Federal do Espírito Santo, 2021-07-07) Quaioto, Bárbara Risse; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/0000-0002-3062-7619; http://lattes.cnpq.br/7948833367111238; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531; Hollais, André Willian; https://orcid.org/0000-0002-2991-8646; http://lattes.cnpq.br/0068789063590867Depression is one of the most common diseases between the cause of global inability, with millions of people affected. It is a heterogeneous and complex disease, which involves several risk factors. Although there is no single theory to explain its development, studies suggest a relationship with lower levels of brain derived neurotrophic factor (BDNF). In this sense, BDNF gene methylation, influenced by environmental factors, may be involved. It is known that individuals with depression are frequently smokers and it has already been demonstrated that changes in BDNF protein levels can occur due to tobacco use. However, the investigation of tobacco effect on BDNF gene methylation has not yet been investigated. Thus, the aim of this study was to evaluate the relationship between depression, smoking and BDNF gene methylation levels. To that end, a cross-sectional study was carried out with individuals assisted by the SUS primary health care network in the municipality of Alegre – ES. Data collection was performed using a socioeconomic, health and lifestyle assessment questionnaire, as well as anthropometric and depression assessment, using the Beck Depression Inventory-II (BDI-II). In addition, blood was collected for genomic DNA extraction and posterior methylation analysis. According to the Poisson regression, the highest prevalence of depression in the sample was explained by the female gender, not having a marriage bond, lack of leisure activities, smoking and BDNF methylation. Mann-Whitney and Generalized Linear Model analysis showed that depressed patients had higher levels of methylation, as well as non-smokers. As demonstrated by the Kruskal-Wallis test, followed by Dunn's posthoc test, smoking habit reduces BDNF methylation levels in depressed individuals, but not in non-depressed individuals. These results provide support for depression prevention policies. Furthermore, the data contribute to the understanding of the pathophysiology and diagnosis molecular of depression, involving the BDNF gene. Additionally, the results expose the need to assess the influence of smoking habit in studies on epigenetic changes in depression.
- ItemASSOCIAÇÃO ENTRE A METILAÇÃO DO GENE NR3C1 E A SÍNDROME METABÓLICA EM ADULTOS ATENDIDOS PELO SUS(Universidade Federal do Espírito Santo, 2022-08-01) Olinda, Amanda Sgrancio; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/0000000307176933; http://lattes.cnpq.br/; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531; Freitas, Flavia Vitorino; https://orcid.org/0000-0003-3722-9987; http://lattes.cnpq.br/0564698519017379Metabolic syndrome (MS) is characterized by a set of risk factors, such as excess abdominal fat, low HDL cholesterol levels, high triglycerides, high blood pressure and high glucose. Due to its complexity and multifactorial characteristics, in addition to environmental factors and genetics, epigenetics may also be involved in the development of metabolic syndrome. Therefore, the objective of this study was to evaluate the determining factors of MS and NR3C1 gene methylation in the promoter region (1F) and to investigate its relationship with SM. Thus, a cross-sectional study was carried out with 353 adult volunteers assisted by SUS. Data collection was carried out using a socioeconomic, lifestyle and, health status questionnaire. Anthropometric data, blood pressure measurements and blood samples were also collected for biochemical and molecular analyses. The extracted DNA was amplified by polymerase chain reaction (PCR) and then subjected to pyrosequencing to determine the methylation profile of the NR3C1 gene. According to Poisson's multivariate analysis with robust variance, the determining factors for metabolic syndrome (p<0.05) were body fat, VLDL cholesterol, and methylation in CpGs 6, 8, 10, and 12 of the NR3C1 gene. In addition, the prevalence of metabolic syndrome in the sample was 23.22%. The data found in this research reinforce the possible epigenetic involvement of the NR3C1 gene with metabolic syndrome.
- ItemAvaliação epigenética do gene BDNF e a relação com a insegurança alimentar e nutricional em usuários do SUS(Universidade Federal do Espírito Santo, 2023-08-15) Luz, Thaiany Marcelino; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/0000-0002-6740-9279; http://lattes.cnpq.br/9121846340652906; Barbosa, Wagner Miranda; https://orcid.org/0000-0002-8712-983X; http://lattes.cnpq.br/9237633153069911; Arantes, Lidia Maria Rebolho Batista; https://orcid.org/0000-0001-8230-1218; http://lattes.cnpq.br/2019308149950531Fear or concern about providing nutritionally for oneself and one's family are inherent to the condition of food and nutritional insecurity (INSAN) experienced by a socially vulnerable portion of the population. INSAN can establish itself as a chronic stress factor, triggering changes in the epigenome of genes involved in the response to stress and neurobiological development. Recent studies have shown that brain-derived neurotrophic factor (BDNF), a gene involved in neurodevelopment, can undergo epigenetic changes, such as DNA methylation, altering its expression. However, there are few studies on the effect of INSAN associated with stress on methylation of the BDNF gene. The objective of this study was to analyze the relationship between INSAN and BDNF gene methylation levels in adults. To this end, a cross-sectional study was carried out, with 379 individuals aged between 20 and 59 years old treated by the SUS basic health care network in the city of Alegre – ES. Data collection was carried out using a socioeconomic, health and lifestyle assessment questionnaire, as well as anthropometric assessment and food and nutritional insecurity situation, using the Brazilian Food Insecurity Scale (EBIA). Blood was collected for genomic DNA extraction and subsequent methylation analysis by pyrosequencing. The prevalence of INSAN was 41.7% in the analyzed sample. According to Poisson regression, the higher prevalence of INSAN in the sample was explained by stress (IRR=1.49), depression (IRR=1.59), leisure activity (IRR=0.75), housing (IRR= 1.53), education (IRR=0.75), per capita income (IRR=0.70) and self-reported health (IRR=1.34). Generalized Linear Model analyzes demonstrated that food-secure individuals had higher methylation levels when compared to food-insecure individuals (β = 0.014; pvalue=0.027). These results demonstrate the importance of observing how an external psychosocial factor, which is related to a pertinent socioeconomic issue, can act and cause changes at a biological level in a vulnerable population.