Mestrado em Bioquímica

URI Permanente para esta coleção

http://repositorio.ufes.br/handle/10/17562

Nível: Mestrado Acadêmico
Ano de início:
Conceito atual na CAPES:
Ato normativo:
Periodicidade de seleção:
Área(s) de concentração:
Url do curso:

Navegar

Navegando Mestrado em Bioquímica por Assunto "anticorpo"

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Nanosensor baseado em ressonância plasmônica de superfície localizada para detecção rápida de glifosato em amostras ambientais
    (Universidade Federal do Espírito Santo, 2021-12-20) Côco, Ariany Soares; Oliveira, Jairo Pinto de; https://orcid.org/0000000175951183; http://lattes.cnpq.br/2228283301316218; https://orcid.org/0000-0001-7497-1625; http://lattes.cnpq.br/1351769143023984; Guimarães, Marco Cesar Cunegundes; https://orcid.org/0000000321460180; http://lattes.cnpq.br/0261991057482057; Antunes, Paulo Wagnner Pereira; http://lattes.cnpq.br/5259147170249880
    Pesticides are among the main chemical products that are most aggressive to the environment due to their persistence in soil and aquatic environments, their long-term effects on living organisms, in addition to mobility between regions, mostly destroying ecosystems and reaching areas adjacent to agricultural production . Glyphosate [N- (phosphonomethyl) glycine] is a non-selective, broad-spectrum herbicide and is known and marketed worldwide. However, its massive and out-of-control application in agriculture has led to environmental and health-related concerns, as in recent years there have been several studies demonstrating contamination in water, soil and food. Therefore, it is of great importance to develop methods capable of simple and low-cost detection that can show different limits allowed by regulatory bodies. In this work, nanotechnology was applied to the fabrication of a biosensor based on gold nanoparticles from the phenomenon of localized surface plasmon resonance (LSPR). Nanoparticles coupled to an IgY-type anti-glyphosate antibody (AuNp-Ac) were used as a glyphosate recognition element. The developed sensor was successful for detecting glyphosate in a wide linear range from 0,009 ug.L-1 to 10.000ug.L-1 . The biosensor response was stable and reproducible, with results that cover the range of interest according to the acceptable daily intake of 0.5 mg/kg, providing a promising alternative for real-time detection of pesticides.