Mestrado em Biotecnologia

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http://repositorio.ufes.br/handle/10/17567

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Navegando Mestrado em Biotecnologia por Assunto "ADIPOQ"

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    Haplótipos da adiponectina, excesso de peso e alterações na homeostasia glicêmica em adolescentes da Grande Vitória
    (Universidade Federal do Espírito Santo, 2018-08-31) Fonseca, Simone Rodrigues da; Errera, Flávia Imbroisi Valle; Maranduba, Carlos Magno da Costa; Paula, Flavia de
    Obesity is a chronic inflammatory disease characterized by excess fat and has been increasing significantly among children and adolescents leading to various complications in childhood and adulthood, such as: systemic arterial hypertension, impaired glucose intolerance, dyslipidemia and type 2 diabetes. A number of factors contribute to childhood obesity and may be: genetic, environmental, and behavioral. Studies have investigated genetic factors predisposing to obesity, although these factors are still poorly understood. The genes that are candidates for predisposition to obesity are related to the regulation of hunger, energy balance, lipid metabolism and glucose; and differentiation of adipocytes. In this context, the adiponectin gene (ADIPOQ) that regulates insulin sensitivity, and is involved in the inflammatory response and regulation of energy balance, represents an interesting opportunity to identify the primary genetic determinants involved in the susceptibility to obesity and diabetes in children and adolescents, since these are one of the main target groups for strategies for research, prevention and control of overweight and obesity. The objective of this study was to analyze the frequencies of genotypes and / or haplotypes to verify the polymorphism in the ADIPOQ gene and its association with the susceptibility to excess weight and alterations of the glycemic homeostasis of adolescents enrolled in public schools in the Metropolitan Region of Vitória-ES, from a representative sample of the study "Prevalence of overweight and obesity in adolescents in the state of Espirito Santo and his association with some variables of the metabolic syndrome "were randomly included in the genetic study. For classification of nutritional status, the body mass index / age (BMI / I) index was considered in z score. Genomic DNA was extracted from peripheral blood samples. For the ADIPOQ gene, the DNA was amplified by Taqman methodology. Data from the individuals related to race, gender, age, BMI / I, glycemia, insulin and HOMA-IR (Homeostasis Model Assessment-Insulin Resistance) were analyzed in SPSS software version 23.0. A total of 350 adolescents aged 10-14 years were investigated, divided into two groups: overweight (n = 109) and no overweight (n = 241), with fourth SNPs in the ADIPOQ gene rs17300539 (-11391 G); 266729 (- 11377C); rs2241766 (45T> G) and rs1501299 (276G> T). For SNP (+ 45T> G), we observed association of genotypes with excess weight (p = 0.04), and association for HOMA β% (p = 0.028). Associations of the ADIPOQ gene haplotypes in the coding region of the ADIPOQ + 45T> G and + 276G> T gene were associated with increased serum glucose levels. We conclude that polymorphism and / or haplotypes TG in the ADIPOQ gene is related to susceptibility to excess weight and changes in adolescent glycemic homeostasis in an age group between 10 - 14 years.
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    Polimorfismos no gene da adiponectina como biomarcadores de transtorno neurocognitivo em idosos
    (Universidade Federal do Espírito Santo, 2021-03-04) Quirino, Karoliny Gomes; Errera, Flavia Imbroisi Valle; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; https://orcid.org/0000-0003-3874-7444; http://lattes.cnpq.br/9954430320767959; Naslavsky, Michel Satya; https://orcid.org/0000-0002-9068-1713; http://lattes.cnpq.br/5084104783599564; Paneto, Greiciane Gaburro; https://orcid.org/0000000180354199; http://lattes.cnpq.br/8176374147579841; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683
    Adiponectin (ADPN), expressed by the ADIPOQ gene, is one of the most abundant adipokines in the body, being a protein hormone that modulates several metabolic processes, including the regulation of blood glucose. It has antiinflammatory and antioxidant function. It acts in insulin sensitization, being one of the protective factors for some diseases such as obesity, diabetes and metabolic syndrome, in which inflammation is one of the central events. Recent evidence suggests that low plasma levels of ADPN can lead to the development of Neurocognitive Disorder (TNC) and dementia, its severe form, through neuroinflammation, obesity and or insulin resistance. Its recently discovered action on neural zones suggests potential benefits for memory and synaptic plasticity.Thus, the hypothesis is that the SNPs in the ADIPOQ gene are associated with the development of TNC and in its most severe form, dementia, and therefore, are good genetic biomarkers of TNC risk. In this context, the objective of this work was to verify whether the polymorphisms are associated with TNC / dementia, anthropometric, biochemical and clinical parameters.1118 participants in the SABE study (Health, Well-Being and Aging) were selected, with complete data on age, sex, biochemical tests, anthropometry, type 2 diabetes, stroke and Mini Mental State Examination (MMSE). Patients who scored below the cut-off line (13) of the MMSE were considered to have TNC (N = 144) and of these, those who scored above the cutoff point (5) in the Pffefer Questionnaire for Activities were considered Functional (N = 111) and below considered as having Light TNC (N = 33). SNPs rs17300539 (11391G> A); rs 266729 (11377C> G); rs 2241766 (45T> G) and rs 1501299 (276G> T) of the ADIPOQ gene were evaluated in an association study. Of the total number of participants, 12.9% (N = 144 patients) had TNC, being 111 with Major TNC or Dementia (111/1118 - 10%) and 33 with Light TNC (33/1118 - 3%). SNPs 11377C >G (rs266729 P = 0.021) and 276G> T (rs1501299 P = 0.016) are associated with the TNC when considering age. Stronger associations were related to increasing age through obesity for SNPs 11377C> G (rs266729; P = 0.005), 113910G> A (rs17300539; P = 0.045) and 276G> T (rs1501299 P = 0.030). Other associations have been identified. We conclude that the development of TNC is associated with SNP in the ADIPOQ gene and that TNC has a genetic architecture related not only to aging, but also to metabolism.