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- ItemUso da tomografia computadorizada de feixe cônico modificada para avaliação padronizada em tecidos ósseo e gengival na reabilitação com implantes imediatos(Universidade Federal do Espírito Santo, 2019-03-12) Bridi, Márjorie das Posses; Kano, Stefania Carvalho; Silva, Daniela Nascimento; Vaz, Sergio Lins de Azevedo; Xible, Anuar AntonioIntroduction: The use of imaging exams to evaluate aesthetic parameters in dental rehabilitation with dental implants is still incipient. Purpose: To evaluate the preoperative dentogengival unit and changes in the bone and gingival tissues after rehabilitation with implants and immediate provision by the technique Soft Tissue Cone-Beam Computed Tomography (ST-CBCT) and to compare the clinical and tomographic characteristics of the gingival tissue. Materials and Methods: This is a series of cases of 06 patients submitted to immediate and temporary implant rehabilitation in the aesthetic maxilla region. The following measurements were made on the vestibular aspect of the images obtained by the ST-CBCT in the pre and postoperative period of 03 and 06 months: Bone Thickness (EO), Bone Height (AO), Gingival Thickness (EG) and Gingival Height (AG). The clinical gingival biotype obtained by periodontal probing was compared to tomographic EG. Data were submitted to descriptive analysis. Results: An increase was recorded EO increased in patients 01 (+1.03mm), 02 (+0.66mm) and 06 (+0.06mm) and reduced in cases 03 (-0.17mm), 04 (-0.41mm) and 05 (-0.38mm). In patients 02 and 03, after bone remodeling with AO reduction, the buccal bone was at the level of the implant platform; in all other cases the implants remained infra-bony. The EG increased in cases 01 (+0.30 mm), 02 (+0.80 mm), 03 (+0,68mm) and 06 (+0.74mm), and there was a reduction in AG of cases 01 (-0.30 mm), 03 (-0.04mm) and 05 (-0.06mm). Conclusions: ST-CBCT has been shown to be a noninvasive instrument for evaluating bone and gingival tissues in the pre and postoperative rehabilitation with immediate implants and allowed standardized measurement of peri-implant tissues in the long term.
- ItemAvaliação da condição periodontal e da microbiota subgengival em adolescentes tratados ortodonticamente com aparelho fixo(Universidade Federal do Espírito Santo, 2019-01-21) Binda, Caroline Lopes; Feitosa, Alfredo Carlos Rodrigues; Guerra, Selva Maria Gonçalves; Gonçalves, Lorena da Rós; Pacheco, Maria Christina ThoméIntroduction: Plaque retention around orthodontic brackets may change subgingival microbiota composition and lead to gingival inflammation and, subsequently, to destruction of supporting periodontal tissues. Objective: To evaluate and compare the periodontal health and subgingival microbial plaque composition after orthodontic treatment. Materials and Methods: 23 participants aged between 10 and 19 years who had finished orthodontic treatment were selected by means of a nonprobabilistic sampling for convenience method. Subgingival plaque samples were obtained from six index teeth (16, 11, 26, 36, 31 and 46) upon orthodontic brackets removal (T1) and after 6 months (T2), and analyzed by the Checkerboard DNA-DNA hybridization technique; in addition, the participants were submitted to clinical examination in terms of visible plaque index, gingival bleeding index, probing depth and bleeding on probing. Results: A significant reduction of periodontal clinical parameters was observed six months after brackets removal. Also at T2, a significant increase of mean counts and proportions of the bacteria C. showae, F. periodonticum, S. sanguinis, S. gordonii, C. gingivalis, A. odontolyticus I, A. naeslundii I was observed (p<.05), as well as a significant reduction of F. nucleatum (sp. vincentii), E. saburreum, S. anginosus e P. melaninogenica (p<.05), with higher frequency for bacteria from the orange complex (T1: 28.28%; T2: 27.88%). Conclusion: Periodontal health is negatively influenced by the use of fixed orthodontic appliances; however, clinical parameters tend to return to normal values after brackets removal as well as the concentration of periodontopathogenic bacteria present in subgingival plaque.
- ItemAvaliação dimensional da via aérea superior e dos seios da face em mulheres com apneia obstrutiva do sono(Universidade Federal do Espírito Santo, 2018-11-27) Silva, Cassiana Augusta da; Brandão, Roberto Carlos Bodart; Pacheco, Maria Christina Thomé; Pereira, Rowdley Robert Rossi; Vaz, Sergio Lins de AzevedoObstructive sleep apnea (OSA) presents with repetitive upper airway obstruction during sleep, daytime sleepiness, decreased quality of life and severe systemic consequences. Even though OSA has a significant prevalence in the general population, many cases remain undiagnosed - mainly in woman - because some disease’s mechanisms are still unknown. The objectives were to evaluate the upper airway and facial sinus dimensions of women with and without OSA to detect possible dimensional changes that could indicate the presence of OSA and to evaluate the influence of age on the dimensions studied. Volumetric and linear measurements were taken in twenty-nine cone beam Computer tomography, in which 16 women had mild and moderate OSA and 13 women had no risk for developing the disease. Volumes evaluated were pharynx (Vfar), nasal complex (Vnas), maxillary sinuses (Vsm), frontal sinus (Vsf) and the sum of all these volumes (Vtotal). Linear measurements were nasal cavity’s width, inferior turbinate’s and soft palate’s length and width. Both groups were evaluated by age of 30-49 and 50-65 years old. No significant differences were found in volumes between groups. However, women with OSA showed a reduction on all the volumes of pneumatic cavities and increased soft palate measurements. The older group with OSA showed a significant increase in the inferior turbinate’s width. Women, in general, showed decreased volumes of facial cavities and increased measurements for lower nasal turbinate and soft palate, although not necessarily caused by the OSA.
- ItemEfeito microbiológico, índice de placa bacteriana, pigmentação dentária e percepção gustativa da clorexidina 0,12% com sistema antidescolorante, após exodontia de terceiros molares(Universidade Federal do Espírito Santo, 2018-12-04) Milleri, Daniela Pertel; Castro, Martha Chiabai Cupertino de; Silva, Daniela Nascimento; Cançado, Renata Pittella; Guimarães, Jackeline CoutinhoChlorhexidine (CHX) is an antiseptic widely used for the chemical control of bacterial plaque and as antimicrobial agent during the postoperative period of oral surgeries; its long-term use may induce adverse effects such as tooth staining, oral mucosa irritation and taste alterations. Objective: to evaluate the plaque index (PI), tooth color change (ΔE), microbiological effect and patients’ perception regarding the use of 0.12% CHX with Anti Discoloration System after third molar extractions. Methods: this prospective cross-over study involved 22 participants that used 0.12% CHX mouthwashes with and without ADS for 15 days each, with an interval of at least 15 days. Silness and Löe PI was recorded on central incisors and first molars; the color of central incisors was measured with a spectrophotometer and ΔE was calculated by using the formula ΔE=[(ΔL*)2+(Δa*)2+(Δb*)2 ] 0,5; the Streptococcus sp in saliva were quantified through Colony Forming Units (CFU); the patient's perception was obtained with the Visual Analogue Scale (VAS) for the following parameters: 1) taste of the product, 2) alteration in food taste, 3) changes in salt perception, 4) irritation of the oral mucosa. Results: a reduction of IP was observed for both groups, with no significant difference between incisors and canines (p>.05); however, molars presented higher IP for the CHX with ADS group (p=.011). ΔE for CHX with and without ADS groups were 2.95 and 3.85, respectively. A significant decrease in CFU/mL was found for both groups, albeit lower for CHX with ADS (p=.001). In the CHX with ADS group, alterations in food taste, changes in salt perception and irritation of the oral mucosa were significantly lower than CHX group (p<.05). Conclusion: both solutions were efficient in bacterial plaque control; ΔE was clinically unsatisfactory for CHX group and acceptable for CHX with ADS; the use of CHX without ADS resulted in significant reduction of CFU/mL; and participants reported less alteration in food taste, salt perception and irritation of the oral mucosa when CHX with ADS was used.
- ItemAnálise de aspectos morfológicos e da expressão de timp-1 na cartilagem articular da mandíbula em condição de má oclusão experimental(Universidade Federal do Espírito Santo, 2018-04-20) Mathias, Carolina Brioschi; Bautz, William Grassi; Bautz, Leticia Nogueira da Gama de Souza; Pacheco, Marcos da Silva; Vaz, Sergio Lins de AzevedoBackground: The temporomandibular joint (TMJ) can be affected by the same pathological process as the other joints in the human body. Osteoarthritis (OA) is the most common temporomandibular disorder and it is characterized by the degeneration of the cartilage tissue and even the bone below. OA has a complex etiology and disordered occlusion is considered a risk factor because it can create a compression of the cartilage tissue leading to the expression of various cytokines and chemokines, such as the matrix metalloproteinases which activity is regulated by the tissue Inhibitors of the metalloproteinases (TIMP). Among the four existing types of TIMP, TIMP-1 is the most important because it can act as a signaling molecule independent of MMP inhibition thereby influencing important biological processes. Aim: To investigate the responses of mandibular condylar cartilage to experimentally induced disordered occlusion and to evaluate changes in the expression of the TIMP-1 molecule. Methods: Twenty-four female Wistar rats at the age of 8 weeks were enrolled in this study. The animais were randomly divided into experimental and control groups and further divided into four subgroups for two time points (2 and 4 weeks). Experimentally induced sagittal disordered occlusion were created by moving the first molars mesially and distalizing the third molars unilaterally and in opposite sides of the dental arches. At the end of two and four weeks, remodeling of the mandibular condylar cartilage was assessed. Protein expression of TIMP-1 were investigated by means of immunohistochemical staining. The quantitative analysis were made through an image software and statistical analysis by chi-square and Mann-Whitney tests were performed. The statistical significance was defined as P< 0.05. Results: In the 2- and 4-week experimental groups OA-like changes were observed. The most common changes were the increased thickness of the posterior third, disarrangement of the layers disposition, osteoclastic activity and osteophyte formation. Also were found cellular alterations that took form of pyknotic nuclei and condensed cytoplasm chondrocytes and characterize diminution of metabolic activity. The TIMP-1 expression was shown in the mature layers of the control group. However, in the experimental group, immunopositive cells were found in the proliferative and mature layers being that the posterior third of the 2-week experimental group presented a higher levei of TIMP-1-positive cells when compared to the correspondent control (P= 0.0291). Conclusion: The present results indicate that the experimentally created disordered occlusion led to degenerative responses accompanied by changes in the expression of TIMP-1 in mandibular condyle cartilage.