Doutorado em Biotecnologia RENORBIO

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    Desenvolvimento de coração bioartificial a partir de arcabouço de matriz extracelular descelularizada enriquecida com fibronectina plasmática humana
    (Universidade Federal do Espírito Santo, 2023-02-27) Taufner, Gabriel Henrique; Nogueira, Breno Valentim; https://orcid.org/0000000221990635; http://lattes.cnpq.br/0011229320439147; https://orcid.org/0000-0002-4040-5948; http://lattes.cnpq.br/4425677222076255; Baldo, Marcelo Perim; https://orcid.org/0000-0002-7673-3580; http://lattes.cnpq.br/7820422119282248; Oliveira, Jairo Pinto de; https://orcid.org/0000000175951183; http://lattes.cnpq.br/2228283301316218; Prado, Adilson Ribeiro; https://orcid.org/0000-0001-8808-4488; http://lattes.cnpq.br/3085491325255749; Diaz, Camilo Arturo Rodriguez; https://orcid.org/0000000196575076; http://lattes.cnpq.br/2410092083336272
    The reconstruction of complex organs after decellularization presents as the greatest challenge the delivery, adhesion, proliferation and differentiation of cells. To succeed in the process, it is necessary to mimic the in vivo microenvironment as much as possible. It is known that decellularization is capable of removing important biomolecules in the morphophysiology of the organ, such as glycosaminoglycans and glycoproteins such as fibronectin. Fibronectin remaining in the extracellular matrix after the decellularization process may not be sufficient to satisfactorily promote the tissue reconstruction process, especially if the scaffold is in the adult stage of development, where the composition of said glycoprotein is known to be reduced. In our study, we obtained decellularized scaffolds to investigate the influence of human plasma fibronectin on adult mouse heart reconstruction. We demonstrated that the decellularized scaffolds were considerably preserved in terms of their matrisomal biomolecular composition. We emphasize the maintenance of the hydroxyproline molecule, found in a concentration of 5,509 ± 818.13 vs. 4,881 ± 1,487 µg/total dry weight in decellularized adult organ and control respectively. Regarding neonatal decellularized scaffolds, we observed maintenance of hydroxyproline when compared to its native control (330.5 ± 17.60 vs. 273.9 ± 12.30 µg/total dry weight). In addition to matrisomal biomolecules, we emphasize the remarkable reduction of DNA (83.63% and 93% in neonatal and adult respectively) and residual SDS (33.92% and 96.44% in neonatal and adult respectively), interfering with the reconstruction process . We investigated the use of a non-destructive analytical approach for decellularized tissues: Raman spectroscopy, whose results corroborated the spectrophotometric analyzes of matrisomal biomolecules. We established an effective strategy for the recomposition of fibronectin in adult decellularized scaffolds, however, we found that despite the success in the recomposition of the glycoprotein, we did not find statistical significance after the reconstruction of the organs from cells of the H9c2 lineage. Given this last finding, we suggest additional studies capable of investigating other classes of fibronectin. Furthermore, it is also necessary to investigate cell populations beyond the H9c2 lineage.
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    Padrões alimentares processados e ultraprocessados e a relação com a metilação dos genes BDNF e NR3C1 e o estado nutricional
    (Universidade Federal do Espírito Santo, 2023-02-24) Vieira, Tamires dos Santos; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108; https://orcid.org/0000000238993664; http://lattes.cnpq.br/9014510901567961; Nogueira, Breno Valentim; https://orcid.org/0000000221990635; http://lattes.cnpq.br/0011229320439147; Hollais, André Willian; https://orcid.org/0000-0002-2991-8646; http://lattes.cnpq.br/0068789063590867; Gouvea, Sonia Alves; https://orcid.org/000000015180471X; http://lattes.cnpq.br/7268228122543743
    The change in the lifestyle of the population with the acquisition of unhealthy habits, such as the industrialized diet, have contributed to the growth of obesity, increase in the prevalence of diabetes, hypertension and other non-transmissible chronic diseases such as depression and cancer. Diet composition can act directly as a transcription factor, in addition to influencing epigenetic modifications, altering gene expression and contributing to the risk of diseases which can be transferred to offspring. Thus, the aim of this study was to evaluate the nutritional status and the relationship between adherence to processed and ultra-processed dietary patterns and levels of methylation of the BDNF and NR3C1 genes. This cross-sectional study collected data from 250 individuals between 20 and 59 years of age, using as indicators the Body Mass Index (BMI), HDL-C and LDL-C for dyslipidemia, TyG index for insulin resistance and symptoms suggestive of depression by the Beck Depression Inventory. The independent variables were socioeconomic factors (gender, age, education, location and income), psychosocial factors (stress, anxiety, depression and food insecurity), nutritional status (TyG Index, HDL-c, LDL-c, BMI, cortisol) and lifestyle (physical activity, smoking and alcoholism) used to adjust the generalized linear model (GLzM). The BDNF and NR3C1 genes methylation profile was evaluated by the pyrosequencing method. Dietary patterns were defined by factor analysis that assessed the interrelationship between commonly consumed foods, resulting in three patterns: healthy, industrialized and mixed. For the methylation of the BDNF sitespecific CpGs, it was decided to work separately, while for the NR3C1 CpGs, the main components were extracted, obtaining two components of CpGs. Comparisons of medians, Spearman correlations and linear models were used to assess the association between BDNF methylation and the 1F region of the NR3C1 gene. The prevalence of overweight and obesity was 33.5% and 39.9%, respectively. Furthermore, 64.9% of the individuals had insulin resistance, and more than 70.0% had an increased risk for metabolic complications related to abdominal adiposity, waistto-height ratio and conicity index. The present study showed that adherence to an unhealthy eating pattern and excess weight played a significant role in BDNF gene methylation, increasing the percentage of methylation (p<0.05). In addition, the lower methylation in the total segment and in component 1 (CpG 44-47) of NR3C1 can be explained by overweight and adherence to the industrialized pattern, when controlled for covariates. And this evidence helps to understand the epigenetic role of adherence.
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    Busca de biomarcadores para a doença de Alzheimer
    (Universidade Federal do Espírito Santo, 2023-02-08) Almeida, Jucimara Ferreira Figueiredo; Paula, Flavia de; https://orcid.org/0000000186792982; http://lattes.cnpq.br/7913201450663683; https://orcid.org/0000000171044623; http://lattes.cnpq.br/6845055093738085; Maranduba, Carlos Magno da Costa; https://orcid.org/0000-0001-7327-1934; http://lattes.cnpq.br/4763153859701731; Errera, Flavia Imbroisi Valle; https://orcid.org/0000-0002-8069-6372; http://lattes.cnpq.br/9337327437538048; Nishimura, Agnes Lumi; https://orcid.org/0000-0001-5295-797X; http://lattes.cnpq.br/4422870765994649; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108
    Alzheimer's disease (AD) is one of the most common forms of dementia in the population and currently in Brazil there are more than 1.2 million individuals with AD. In AD there is death of neurons and loss of synapses, inflammatory processes and brain atrophy caused by the formation of amyloid plaques and tangles of neurofibrils. In 98% of the cases, AD is sporadic, and the APOE gene acts as the most risk factor. The pathophysiological process of AD begins many years before clinically evident symptoms. In this sense, the identification of genetic biomarkers would allow for a less invasive, more accurate diagnosis with a more accessible value, in the same way it could be used as a marker of progression and response to treatment. Thus, the main objective of this work was to search for potential AD biomarkers by studying polymorphisms in the ABCA7 (rs3764650), CR1 (rs6656401), BIN1 (rs744373), CLU (rs11136000) and MS4A6A (rs610932) genes in a meta-analysis. In addition, an association study of the CASS4 (rs911159), TREM2 (rs75932628), CD2AP (rs9349407) and MS4A4E, (rs670139) gene variants and a combined risk study of the APOE (rs429358 and rs7412), BIN1 (rs744373) genes, ABCA7 (rs3764650) and CLU (rs11136000). The results of the meta-analysis validated the risk of BIN1, CR1, ABCA7 gene variants, as well as the protective effect of MS4A6A and CLU. In the combined risk study, the results suggest a risk effect for AD between APOE with CLU and APOE with the BIN1 gene. In the case-control study, there was a positive association of the rs911159 polymorphism of the CASS4 gene with Alzheimer's disease. These results, in addition to validating GWAS studies in a poorly studied population, such as the Brazilian population, were important to broaden the understanding of the disease and increase the diversity of populations studied in the world in genetic studies for AD. In addition, this work opens the way for new studies in search of early diagnostic biomarkers for sporadic Alzheimer's disease.
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    APPLICATION OF GREEN NANOPARTICLES AND NANOCELLULOSE IN THE PRODUCTION OF SMART TEXTILES
    (Universidade Federal do Espírito Santo, 2022-08-31) Silva, Laryssa Pinheiro Costa; Guimaraes, Marco Cesar Cunegundes; https://orcid.org/0000000321460180; http://lattes.cnpq.br/0261991057482057; https://orcid.org/; http://lattes.cnpq.br/; Dutra, Rosa Amalia Fireman; https://orcid.org/; http://lattes.cnpq.br/; Ventura, Jose Aires; https://orcid.org/0000000314221739; http://lattes.cnpq.br/8687116881326074; Nascimento, Diego Magalhaes do; https://orcid.org/; http://lattes.cnpq.br/
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    PERFIL CLÍNICO, MICROBIOLÓGICO E RESPOSTA IMUNE DA PERIODONTITE E SEU IMPACTO EM MULHERES COM CÂNCER DE MAMA
    (Universidade Federal do Espírito Santo, 2021-12-20) Gomes, Maria da Conceicao Machado; Rangel, Leticia Batista Azevedo; https://orcid.org/0000000261504064; http://lattes.cnpq.br/6706127386696883; https://orcid.org/; http://lattes.cnpq.br/; Guimaraes, Marco Cesar Cunegundes; https://orcid.org/0000000321460180; http://lattes.cnpq.br/0261991057482057; Reis, Priscila Ladeira Casado; https://orcid.org/; http://lattes.cnpq.br/; Facanha, Arnoldo Rocha; https://orcid.org/; http://lattes.cnpq.br/; Silva, Adriana Madeira Alvares da; https://orcid.org/0000000280780304; http://lattes.cnpq.br/6445492335035108
    The main objective this study was to evaluate the subgingival microbial profile and levels of inflammatory markers of periodontitis associated with breast cancer (BC) and the impact of periodontal therapy on these findings. This study evaluated in 2 phase