Estudo da produção e caracterização parcial de avicelase e carboximetilcelulase de Bacillus licheniformis CCMA – 1674 em meio de cultivo contendo casca de café conilon tratada com NaOH e ultrassom
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Data
2023-02-10
Autores
Alves, Thálita Cristyne de Oliveira
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Universidade Federal do Espírito Santo
Resumo
Biotechnological processes allow the production of large amounts of metabolites with high added value and industrial application, such as, for example, cellulolytic enzymes. These enzymes can be produced by microorganisms in culture media containing agro-industrial residues, which can be found in abundance throughout Brazil. In this context, the objective of this work was to study the optimal conditions for the cultivation and production of cellulases by the bacteria Bacillus licheniformis CCMA-1674, in medium containing conilon coffee husks. They were treated with an alkaline solution of NaOH at 1% (w/v) and subsequently with ultrasound for 20 min at 30°C and 40 KHz. Bacillus licheniformis CCMA-1674 was able to produce avicelase and carboxymethylcellulase in a medium with 1.0% Conilon coffee hulls treated with NaOH and US and 0.3% Whey protein (ISOFORT® Vitafor), during 120h at 37 oC and 150 rpm. Avicellase showed optimal activity at pH 5.0 and temperature of 40 °C (2.296 U/mL), while carboxymethylcellulase showed optimal activity at pH 7.0 and temperature of 90 °C (12.640 U/mL). There was an increase of more than 100% in the avicelase activity in the presence of the Mn2+ ion at temperatures of 50, 70 and 90 oC during 120 min. And carboxymethylcellulase activity was 25 times greater when the enzyme was incubated at 50 oC and in the presence of Fe2+.
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Celulases , Bacillus , Resíduo lignocelulósico , Café conilon , Tratamentos de resíduos