Mestrado em Ciência e Tecnologia de Alimentos
URI Permanente para esta coleção
Nível: Mestrado Acadêmico
Ano de início: 2011
Conceito atual na CAPES: 4
Ato normativo: Homologado pelo Parecer CES/CNE nº 487/2018 (Portaria MEC nº 609, de 14/03/2019) e Publicado DOU em 18/03/2019 (Seção 1, pág. 63).
Periodicidade de seleção: Semestral
Área(s) de concentração: Ciência e Tecnologia de Alimentos
Url do curso: https://cienciaetecnologiadealimentos.ufes.br/pt-br/pos-graduacao/PPGCTA/detalhes-do-curso?id=1076
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- ItemBiocontrole de fungos toxigênicos isolados de produto cárneo curado à seco(Universidade Federal do Espírito Santo, 2025-02-17) Martins, Pedro Henrique Alves; Bernardes, Patrícia Campos ; https://orcid.org/0000-0002-0012-3890; http://lattes.cnpq.br/0443413246742848; http://lattes.cnpq.br/1756921355251468; Rosário, Denes Kaic Alves do; https://orcid.org/0000-0001-8565-2021; http://lattes.cnpq.br/7963921448042379; Conte Júnior, Carlos Adam ; https://orcid.org/0000-0001-6133-5080; http://lattes.cnpq.br/6146781658944580; Santos, Mário Ferreira ConceiçãoCured meats are traditional products widely consumed in various cultures, recognized for their complex flavors and economic importance. The production of these foods involves processes such as salting, drying, and fermentation, which promote the development of a complex microbiota, including lactic acid bacteria, yeasts, and filamentous fungi. However, the presence of mycotoxin-producing fungi, especially ochratoxin A (OTA), poses a significant public health risk. The main fungi responsible for contamination in cured meats include Aspergillus westerdijkiae, Penicillium nordicum, and Penicillium verrucosum. This study aimed to investigate the potential of Penicillium nalgiovense, Meyerozyma caribbica, and Hanseniaspora opuntiae as biocontrol agents in inhibiting the growth of toxigenic fungi. Radial inhibition assays were conducted on potato dextrose agar (PDA) and Socol-based agar (SBA), which simulated the initial maturation conditions of meat products. The results demonstrated that P. nalgiovense strains showed the highest efficacy in inhibiting toxigenic fungi at temperatures of 15 °C and 25 °C. OTA quantification was performed using high-performance liquid chromatography with fluorescence detection (HPLC-FLD), revealing significant variation in OTA production among the studied fungi. OTA concentrations ranged from 10.42 µg/kg to 1054.87 µg/kg in PDA and from 5.18 µg/kg to 142.22 µg/kg in SBA, indicating that the composition of the medium influenced mycotoxin production. The findings of this study highlight the relevance of P. nalgiovense as an effective and safe biocontrol agent in cured meat production. Additionally, they emphasize the need to optimize inoculation and maturation conditions to ensure the safety and quality of the final product. Future studies should explore different concentrations and combinations of biocontrol microorganisms, as well as their applications and influences on the sensory characteristics of the product, contributing to the development of effective strategies for mycotoxin control.
- ItemEfeito do extrato aquoso de café verde (Coffea canephora) na saúde intestinal de animais alimentados com dieta hipercalórica e hiperlipídica(Universidade Federal do Espírito Santo, 2024-09-29) Meneguelli, Natália Alves de Souza; Trivilin, Leonardo Oliveira ; https://orcid.org/0000-0002-3039-3269; http://lattes.cnpq.br/5723897284006298; Grancieri, Mariana; https://orcid.org/0000-0001-8888-5496; http://lattes.cnpq.br/6646062052507980; Costa, André Gustavo Vasconcelos ; https://orcid.org/0000-0002-2393-6384; http://lattes.cnpq.br/5406130298857736; https://orcid.org/0009-0007-3247-3004; http://lattes.cnpq.br/9189123775450715; Sant' Ana, Cíntia Tomaz ; https://orcid.org/0000-0002-1385-9274; http://lattes.cnpq.br/5267705234203118; Cintra, Dennys Esper Corrêa ; https://orcid.org/0000-0002-7954-5630; http://lattes.cnpq.br/2329875440704117Inadequate eating habits, such as excessive caloric intake and diets high in saturated fats, are associated with the development of obesity and intestinal alterations. Green coffee is rich in bioactive antioxidant compounds, which may assist in obesity management. However, its effects on intestinal health, particularly in the context of obesity, remain unclear. This study aimed to evaluate the effect of aqueous green coffee extract on intestinal health in an animal model of obesity. A total of 32 adult Wistar rats were divided into four experimental groups (n=8): CT – negative control (commercial control diet without green coffee extract supplementation), HF – positive control (obesity-induced without green coffee extract), HFP – prevention experimental group (obesity-induced with extract supplementation from the first experimental week), and HFT – treatment experimental group (obesity-induced with extract supplementation starting from the 8th week). Obesity was induced over eight weeks through a hypercaloric and high-fat diet (HF) diet. At the end of the 16th week, the animals were euthanized. Colon tissue samples were collected for analysis of oxidative markers (catalase, superoxide dismutase - SOD, and nitric oxide - NO), morphometric parameters and junction protein. Cecal and colonic contents were used to analyze pH, short-chain fatty acids (SCFAs), and immunoglobulin A (IgA). Food intake was monitored daily, and body weight was measured weekly. For statistical analysis, at test was applied between CT and HF groups, while ANOVA followed by Tukey’s post hoc test was used for HF, HFP, and HFT groups, adopting a 5% significance level. Compared to the CT group, the HF group exhibited significantly higher means (p<0.05) for feed and caloric efficiency coefficients, weight gain, and murinometric parameters, confirming the induction of obesity. A significant reduction (p<0.05) in intestinal pH, NO, SOD, morphometric parameters, claudin, and occludin was also observed. However, supplementation with the green coffee extract did not influence parameters related to food intake or murinometric measurements. On the other hand, in the HFP group, the extract prevented mucosal alterations and the reduction of junction proteins, whereas in the HFT group, it promoted a reduction in intestinal pH, an increase in butyrate and acetate levels, morphometric recovery, and increased claudin and occludin quantification. These findings demonstrate the beneficial effects of aqueous green coffee extract on intestinal health, highlighting its potential as a strategy for preventing and treating intestinal alterations induced by a high-fat diet
- ItemAplicação de óxido nítrico na pós-colheita de mamão formosa (carica papaya l.)(Universidade Federal do Espírito Santo, 2025-02-07) Candido, Laura Rocha; Zucoloto, Moises; Carneiro, Joel Camilo Souza; Coelho, Jussara Moreira; https://orcid.org/0000-0002-7641-5638; Ventorim, Rafaela Zandonade; Silva, Pollyanna IbrahimThe papaya (Carica papaya L.) is a tropical fruit belonging to the Caricaceae family. Its ripening process involves intense transformations such as a high respiration rate, softening, and color degradation, which together contribute to the loss of fruit quality. In this context, nitric oxide (NO), an important signaling biomolecule, has been studied as an alternative to delay fruit ripening. Research has shown that the exogenous application of this molecule can help maintain fruit quality due to its high capacity to interact with ethylene, polyamines, γ-aminobutyric acid, and abscisic acid. Additionally, NO directly influences the antioxidant system of fruits, enhancing their defense mechanisms.Given this scenario, an experiment was conducted using sodium nitroprusside (SNP), a nitric oxide donor, to preserve the quality of Formosa papaya fruits. Three replicates were performed, using 15 fruits per replicate, totaling 45 fruits per treatment and 180 fruits in total. The papaya fruits were immersed in solutions of 0, 1000, 2000, and 3000 μmol/L of SNP and subsequently stored at room temperature (25°C) with 50–60% relative humidity for nine days. Samples were collected on days 1, 3, 5, 7, and 9 of storage for physical, physicochemical, and biochemical analyses.The results demonstrated that treatment with 1000 and 2000 μmol/L of SNP delayed firmness loss, whereas control fruits exhibited softening greater than 50% by the third day of storage. Moreover, a reduction in mass loss (%) and an accumulation of antioxidant substances, such as vitamin C and carotenoids, were observed in papayas treated with 1000 and 2000 μmol/L of SNP. Additionally, SNP inhibited the activity of the polygalacturonase (PG) enzyme on the third day of storage, which explains the higher integrity of the fruit cell wall. The enzymes β-galactosidase (β-Gal), FPase (total cellulase), and lipoxygenases (LOXs) were also influenced by the treatment, leading to lower activity throughout storage.Thus, it is concluded that SNP is an effective method for maintaining the quality of Formosa papaya fruits during storage at room temperature (25°C), standing out in the post-harvest stage by preserving the physicochemical properties of the fruits.
- ItemEfeito do extrato de café verde (Coffea canephora) nos biomarcadores metabólicos e na morfologia dos tecidos hepáticos e adiposo, em ratos com obesidade induzida por dieta rica em gordura(Universidade Federal do Espírito Santo, 2024-11-26) Nascimento, Manuella Domiciano do; Costa, Neuza Maria Brunoro ; https://orcid.org/0000-0002-4967-9937; http://lattes.cnpq.br/9592871700382838; Viana, Mirelle Lomar ; https://orcid.org/; http://lattes.cnpq.br/4184974398500192; Costa, André Gustavo Vasconcelos ; https://orcid.org/0000-0002-2393-6384; http://lattes.cnpq.br/5406130298857736; https://orcid.org/0009-0003-0686-0909; http://lattes.cnpq.br/5253101651334775; Sant' Ana, Cíntia Tomaz ; https://orcid.org/0000-0002-1385-9274; http://lattes.cnpq.br/5267705234203118; Cintra, Dennys Esper Corrêa ; https://orcid.org/0000-0002-7954-5630; http://lattes.cnpq.br/2329875440704117Obesity is a global chronic condition characterized by excessive body fat, increasing the risk of diseases such as type 2 diabetes and cardiovascular problems. It is associated with insulin resistance, dyslipidemia, chronic inflammation, and oxidative stress. Foods containing bioactive compounds have shown potential to assist in modulating obesity. Green coffee (Coffea canephora), or unroasted coffee, is rich in bioactive compounds such as chlorogenic acid, caffeine, and trigonelline, which undergo less degradation compared to roasted coffee. Studies indicate that green coffee may offer benefits in reducing oxidative stress and body weight. Thus, this study aimed to evaluate the effect of green coffee extract on metabolic alterations and the morphology of liver and adipose tissues in rats subjected to obesity. The green coffee sample was obtained from producers in the Caparaó Capixaba region. Initially, the major compounds in green coffee were quantified. Forty-eight adult Wistar rats were divided into four groups(n=12): CT group (control commercial diet, no supplementation with green coffee extract), HF group (high-fat, high-calorie diet without green coffee supplementation), HFP group (high-fat, high-calorie diet supplemented with green coffee extract from the first week of the experiment), and HFT group (high-fat, high-calorie diet supplemented with green coffee extract starting in the eighth week of the experiment). The obesity-inducing diet was high-calorie and high fat (5.35kcal/g), consisting of 31.2% pork lard. Green coffee extract supplementation was administered daily, with 2 mL of extract per animal, incorporated into their diet. The animals were monitored daily for food intake and weekly for body weight. In the 15th week, an intraperitoneal glucose tolerance test was performed. In the 16th week, the animals were subjected to 12 hours of fasting and euthanized via cardiac puncture. Blood was collected and centrifuged for metabolic marker analyses. Epididymal adipose tissue and the liver were removed, weighed, and prepared for inflammatory, oxidative, and histological analyses. A t-test was applied to compare control results, and ANOVA followed by Tukey's test (p>0.05) was used for test group comparisons against the positive control. The proposed obesity model was effective, as demonstrated by weight gain, food and caloric intake coefficients, and murinometric parameters. On the other hand, green coffee extract showed no significant effects on food intake, weight gain, glucose intolerance, or oxidative, inflammatory, or adipose tissue morphology parameters. However, the HFT group exhibited lower aspartate aminotransferase concentrations, increased HDL-c levels, and a reduction in hepatic steatosis severity, shifting from severe to moderate. Green coffee extract may benefit liver health in obesity conditions, modulating hepatic markers and attenuating the severity of liver steatosis, indicating its potential as an adjuvant in treating metabolic alterations resulting from excessive calorie and fat intake
- ItemEfeito dos feijões-caupi (Vigna unguiculata (L.) Walp.) biofortificados na biodisponibilidade de ferro e no metabolismo glicídico e lipídico de ratos anêmicos alimentados com dieta rica em gordura e açúcar(Universidade Federal do Espírito Santo, 2024-08-27) Gaspar, Ana Paula Ribeiro; Sant’Ana, Cíntia Tomaz ; https://orcid.org/; http://lattes.cnpq.br/; Grancieri, Mariana ; https://orcid.org/; http://lattes.cnpq.br/; Costa, Neuza Maria Brunoro ; https://orcid.org/; http://lattes.cnpq.br/; https://orcid.org/; http://lattes.cnpq.br/; Martino, Hércia Stampini Duarte; https://orcid.org/; http://lattes.cnpq.br/; Tostes, Maria das Graças Vaz; https://orcid.org/; http://lattes.cnpq.br/Nutrient and micronutrient deficiencies are a condition with high incidence worldwide, whether due to the lack of a nutritionally balanced diet or excessive consumption of foods rich in fat and sugar and poor in vitamins and minerals. This can cause metabolic and systemic complications, including dysfunctions in iron, glucose and lipid metabolism, predisposing the individual to chronic non-communicable diseases. In this context, biofortified beans emerge as a strategy to mitigate these deleterious effects, attenuating dyslipidemic conditions, markers of the glucose profile and contributing to the fight against iron deficiency anemia. Thus, this study aimed to evaluate the effect of biofortified cowpeas (Vigna unguiculata (L.) Walp.) on iron bioavailability and glucose and lipid metabolism of anemic rats fed a diet rich in fat and sugar. Two biofortified cultivars, BRS Aracê, BRS Tumucumaque and a conventional cultivar, BRS Pajeú, were used. Physicochemical analyses determined the nutritional composition of the beans. Iron bioavailability was assessed by the depletion/repletion method in an animal model (n=60) consuming an AIN-93G diet with 30% lipid content, with lard as the main source and 30% sugar (HFHS diet) or a normal diet (NS). In the depletion phase (21 days), 12 animals received an NS diet and 48 consumed HFHS, both iron free. In the repletion phase (35 days), the animals received 12 ppm of iron from ferrous sulfate (SF) or cowpea. The HFHS group was subdivided into four groups (n=12): HS (HFHS + SF), HA (HFHS + Aracê), HT (HFHS + Tumucumaque) and HP (HFHS + Pajeú). Hemoglobin was evaluated at the end of depletion and after 14 and 34 days of repletion. Histological and biochemical analyses related to lipid, glucose and ferric metabolism were performed. The results were submitted to the unpaired t-test, Analysis of Variance followed by Tukey's test and Pearson's correlation (p<0.05). The project was approved by the Ethics Committee (protocol 013/2022). Tannins were absent in the three genotypes. Aracê had a higher protein content and Pajeú a higher content of soluble fiber, resistant starch, total phenolics and Phytate:Iron ratio. The biofortified crops demonstrated a higher iron content in the grains. All demonstrate iron bioavailability similar to the standard diet. HS by RBV and HRE. HA showed greater hemoglobin gain, lower pH and higher fecal butyrate content than HP and also showed higher HDL-c, lower increase in the area under the glycemic curve and insulin than the HS group. HP had lower glycemia, fasting triglycerides, TyG index and TG/HDL-c ratio. All beans showed lower CT/HDL-c ratio, but lower fecal triglyceride excretion compared to HS. All groups demonstrated the absence of hepatic steatosis. Cowpeas, in general, proved to be good sources of highly bioavailable iron and with promising properties to counteract complications of high fat and sugar consumption