Efeito do extrato aquoso de café verde (Coffea canephora) na saúde intestinal de animais alimentados com dieta hipercalórica e hiperlipídica
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Data
2024-09-29
Autores
Meneguelli, Natália Alves de Souza
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Universidade Federal do Espírito Santo
Resumo
Inadequate eating habits, such as excessive caloric intake and diets high in saturated fats, are associated with the development of obesity and intestinal alterations. Green coffee is rich in bioactive antioxidant compounds, which may assist in obesity management. However, its effects on intestinal health, particularly in the context of obesity, remain unclear. This study aimed to evaluate the effect of aqueous green coffee extract on intestinal health in an animal model of obesity. A total of 32 adult Wistar rats were divided into four experimental groups (n=8): CT – negative control (commercial control diet without green coffee extract supplementation), HF – positive control (obesity-induced without green coffee extract), HFP – prevention experimental group (obesity-induced with extract supplementation from the first experimental week), and HFT – treatment experimental group (obesity-induced with extract supplementation starting from the 8th week). Obesity was induced over eight weeks through a hypercaloric and high-fat diet (HF) diet. At the end of the 16th week, the animals were euthanized. Colon tissue samples were collected for analysis of oxidative markers (catalase, superoxide dismutase - SOD, and nitric oxide - NO), morphometric parameters and junction protein. Cecal and colonic contents were used to analyze pH, short-chain fatty acids (SCFAs), and immunoglobulin A (IgA). Food intake was monitored daily, and body weight was measured weekly. For statistical analysis, at test was applied between CT and HF groups, while ANOVA followed by Tukey’s post hoc test was used for HF, HFP, and HFT groups, adopting a 5% significance level. Compared to the CT group, the HF group exhibited significantly higher means (p<0.05) for feed and caloric efficiency coefficients, weight gain, and murinometric parameters, confirming the induction of obesity. A significant reduction (p<0.05) in intestinal pH, NO, SOD, morphometric parameters, claudin, and occludin was also observed. However, supplementation with the green coffee extract did not influence parameters related to food intake or murinometric measurements. On the other hand, in the HFP group, the extract prevented mucosal alterations and the reduction of junction proteins, whereas in the HFT group, it promoted a reduction in intestinal pH, an increase in butyrate and acetate levels, morphometric recovery, and increased claudin and occludin quantification. These findings demonstrate the beneficial effects of aqueous green coffee extract on intestinal health, highlighting its potential as a strategy for preventing and treating intestinal alterations induced by a high-fat diet
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Obesidade , Conilon , Ácido clorogênico , Ácidos graxos de cadeia curta , Proteínas de junção , Obesity , Chlorogenic acid , Short-chain fatty acids , Unction proteins