Avaliação do potencial neuroprotetor da N-Acetilcisteína sobre parâmetros comportamentais e de estresse oxidativo no modelo experimental de cocaína subaguda em ratos
Nenhuma Miniatura disponível
Data
2018-12-13
Autores
Rebelo, Jamille Caneva Oliveira
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal do Espírito Santo
Resumo
The use of drugs of abuse is considered a serious social-economic problem around the world. Among these drugs, cocaine stands out since recent researches has pointed out an increase in consumption, mainly due to the increase of users in South America. Consumption of this kind of drug has been associated with cognitive impairment. Because of this, studies have sought medicaments in order to reduce /prevent damage to memory, presenting N-acetylcysteine (NAC) as one of than. So, the present work had the objective of investigating the possible effects of pre-treatment with NAC followed by cocaine consumption, on memory and oxidative stress brain protein. Therefore, male Wistar rats were submitted to the object recognition test (ORT) protocol for short-term memory evaluation 1h 30 min after the training session and long-term memory 24h after the training session. At the end of the behavioral protocol the animals were euthanized and the cerebral regions of the prefrontal cortex (CPF) and hippocampus (HPC) were collected for biochemical analysis, such as: reduced glutathione (GSH), reduced glutathione ratio and oxidized glutathione / GSSG), advanced protein oxidation products (AOPP) and protein carbonylation index by densitometry (Oxyblot). Results showed that animals receiving cocaine (10mg / kg) presented short term memory impairment when compared to control animals (p = 0.005). On other hand, animals that received cocaine but were previously treated with NAC showed improvement in long-term memory when compared to animals that were not previously treated with NAC (p = 0.001). The group submitted to pre-treatment with NAC followed by cocaine treatment did not present a significant difference in protein oxidative damage in the prefrontal cortex (CPF) and hippocampus (HPC) when compared to the group previously treated with saline.The main conclusions of the present work point out NAC was able to protect memory recognition
Descrição
Palavras-chave
Recognition memory , Cocaine , N-acetylcysteine , Oxidative stress , Memória de reconhecimento , Cocaína , N-acetilcisteina , Estresse oxidativo