Is the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?

Página do item simplificado
dc.contributor.advisor-co1Carrijo, Tatiana Tavares
dc.contributor.advisor1Fontes, Milene Miranda Praça
dc.contributor.authorSilva, Paulo Marcos Amaral
dc.contributor.referee1Clarindo, Wellington Ronildo
dc.contributor.referee2Gonçalves, Rosado, Carla Cristina
dc.date.accessioned2018-08-01T22:57:30Z
dc.date.available2018-08-01
dc.date.available2018-08-01T22:57:30Z
dc.date.issued2015-08-14
dc.description.abstract
dc.description.resumoThe dioecy occurs in 6% of the angiosperms, including all the Myrsine species (Primulaceae) that show male and female individuals distinguishable based in sexual organs and morphology of sepals and petals. The sexual system fixed in this genus, was the motivation in research if the existence of sexual chromosomes culminate in dioecy for Myrsine. Employing tissue culture, flow cytometry and cytogenetic tools, the aimed this study was characterize the karyotype of Myrsine coriacea (Sw.) R.Br. ex Roem & Schult., Myrsine umbellata Mart., Myrsine guianensis (Aubl.) Kuntze and Myrsine parvifolia A.DC. From leaves of male and female individuals collected in the field and leaves and roots of unknown sex obtained of in vitro culture, were found mean values of DNA content statistically identical. These data were attributed to the presence of secondary metabolites reported for the genus. However, an intraspecific variation was observed in cytogenetic analysis in the four Myrsine species. Slides exhibited metaphases with 2n = 45 and 2n = 46 chromosomes in different individuals. These results were observed consistently from different times of exposition to anti-mitotic agent and distinct treatments of enzymatic maceration. Thus, the chromosome number variation found, associated with the sexual system well defined, can be concerning to the sexual chromosomes in Myrsine genus. These data suggest a chromosomal system of sex 9 determination with multiple X and/or Y described in some plant species. The system ZW also is possible, as well as X0 or Z0, systems still not reported in vegetal groups. The present work provided the first data about the nuclear DNA content by flow cytometry in Myrsine and supplied cytogenetics evidences that indicate the existence of sexual chromosomes.
dc.formatText
dc.identifier.urihttp://repositorio.ufes.br/handle/10/7853
dc.languagepor
dc.publisherUniversidade Federal do Espírito Santo
dc.publisher.countryBR
dc.publisher.courseMestrado em Genética e Melhoramento
dc.publisher.initialsUFES
dc.publisher.programPrograma de Pós-Graduação em Genética e Melhoramento
dc.rightsopen access
dc.subjectCytogeneticpor
dc.subjectFlow cytometrypor
dc.subjectSex chromosomespor
dc.subjectSex determinatpor
dc.subject.br-rjbnCitogenética
dc.subject.br-rjbnCitometria de fluxo
dc.subject.br-rjbnCromossomos sexuais
dc.subject.br-rjbnSexo - Causa e determinação
dc.subject.cnpqMelhoramento Vegetal
dc.subject.udc631.523
dc.titleIs the dioecy in Myrsine (Primulaceae) defined by sex chromosomes?
dc.typemasterThesis
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
tese_9189_Dissertação Final Paulo Marcos Amaral Silva20160628-103338.pdf
Tamanho:
812.74 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
Mestrado em Genética e Melhoramento