Propagação in vitro e ex vitro, aspectos anatômicos e fisiológicos de Neoregelia concentrica (Bromeliaceae)
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Data
2012-02-16
Autores
Martins, João Paulo Rodrigues
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Universidade Federal do Espírito Santo
Resumo
The spread in vitro may contribute to the multiplication of several species of
commercial interest, as bromeliads. However, in vitro culture conditions can induce
the formation of plants with poorly developed structures and tissues that can harm
the subsequent ex vitro establishment. Thus, the objective was to develop a protocol
for the propagation of bromeliad Neoregelia concentrica, taking into account
anatomical and physiological aspects. The first experiment concerning the
multiplication, was conducted from plants of N. concentrica previously established in
vitro and with 180 days old. These were inoculated into test tubes containing MS
medium supplemented with 6-benzylaminopurine (BAP) or kinetin (KIN) at 0.0; 5.0;
10.0 and 15.0 µM. At 60 days were analyzed for agronomic parameters and random
collection of three plants of each treatment for morphoanatomical analysis. The
second experiment, referring to the rooting, was carried out by two methods, in vitro
and ex vitro. In vitro rooting, shoots multiplied with 15.0 µM of BAP were cultured for
60 days on MS medium supplemented with indole-3-butyric acid (IBA) or
naphthalene-acetic acid (NAA) at 0.0; 1.0;, 2.0; 3.0 and 4.0 µM. For ex vitro rooting,
shoots multiplied with 15.0 µM of BAP had their bases immersed for 60 minutes in a
solution of IBA or NAA at 0.0; 5.0; 10.0 and 15.0 µM. After immersion, the shoots
were planted in plastic trays containing vermiculite and grown for 45 days. At the end
of each method of rooting analysis was performed character phytotechnical. In the
third experiment, referring to the acclimatization, plants N. concentrica multiplicated
and rooted in vitro with 15.0 µM of BAP and NAA 3.0 µM, respectively, were planted
in plastic trays containing vermiculite and grown under light levels 30%, 50%, 70%
and 100% for 28 days. The analysis of fluorescence was performed every 7 days and
the evaluation of characters phytotechnical at 28 days of acclimatization. In vitro
multiplication, it was found that increased levels of cytokines induced higher emission
x
of shoots, and the formation of leaf tissues thicker, the use of BAP and more efficient
with regard to the KIN. In the second experiment, we observed a higher rooting in
vitro (30 days) and greater number of roots in shoots grown in medium containing 3.0
µM NAA, already in ex vitro rooting, we observed a higher number of roots and with
greater length mean when applied 5.0 µM IBA. In the acclimatization, it was found
less stress in plants grown under partial reduction of light level, highlighting the level
of 50% due to recovery in less time and greater growth of N. concentrica. It was
possible to establish an efficient protocol for the propagation of bromeliads N.
concentrica.
Descrição
Palavras-chave
Plant anatomy , Bromeliad , Bromélia , Tissue culture , Cultura de tecidos , Chlorophyll fluorescence , Fluorescência da clorofila , Organogênese direta , Direct organogenesis , Tecidos (Anatomia e fisiologia) - Cultura e meios de cultura
Citação
MARTINS, João Paulo Rodrigues. Propagação in vitro e ex vitro, aspectos anatômicos e fisiológicos de Neoregelia concentrica (Bromeliaceae). 2012. 94 f. Dissertação (Mestrado em Agricultura Tropical) - Programa de Pós-Graduação em Agricultura Tropical, Universidade Federal do Espírito Santo, São Mateus, 2012.